In Vitro Cytotoxicity of White MTA, MTA Fillapex® and Portland Cement on Human Periodontal Ligament Fibroblasts

Abstract

The aim of this study was to compare the in vitro cytotoxicity of white mineral trioxide aggregate (MTA), MTA Fillapex ® and Portland cement (PC) on human cultured periodontal ligament fibroblasts. Periodontal ligament fibroblast culture was established and the cells were used for cytotoxic tests after the fourth passage. Cell density was set at 1.25 x10 4 cells/well in 96-well plates. Endodontic material extracts were prepared by placing sealer/ cement specimens (5x3mm) in 1mL of culture medium for 72 h. The extracts were then serially two-fold diluted and inserted into the cell-seeded wells for 24, 48 and 72 h. MTT assay was employed for analysis of cell viability. Cell supernatants were tested for nitric oxide using the Griess reagent system. MTA presented cytotoxic effect in undiluted extracts at 24 and 72 h. MTA Fillapex ® presented the highest cytotoxic levels with important cell viability reduction for pure extracts and at ½ and ¼ dilutions. In this study, PC did not induce alterations in fibroblast viability. Nitric oxide was detected in extract-treated cell supernatants and also in the extracts only, suggesting presence of nitrite in the soluble content of the tested materials. In the present study, MTA Fillapex displayed the highest cytotoxic effect on periodontal ligament fibroblasts followed by white MTA and PC.

Cytotoxicity Evaluation of Root Canal Sealers Using an In Vitro Experimental Model with Roots

“…As it was corroborated by the SEM results in the same period, it is possible to notice some free areas or some cells with circular morphology. Probably, this effect is related to the addition of components to MTA Fillapex, such as resin composites like the salicylate (14) or other pigments, which may have increased the toxic effect, because in contact with cells, these soluble chemicals may lead to cell modification (24).…”

Assessment of intracanal medications cytotoxicity on L929 fibroblast cells

“…Some studies (CHANG et al, 1998;CAMARGO et al, 2009;SEPET et al, 2009;SILVA et al, 2012A;SILVA et al, 2012B;YOSHINO et al, 2013) used fibroblasts for quick and easy growth (HIRSHMAN et al, 2012), in addition to their availability in periapical tissues and the periodontal ligament, which are areas that are susceptible to the effects of intracanal medications and their degradation products (YOSHINO et al, 2013). Fibroblasts are also the largest producers of collagen tissue and, therefore, actively participate in the repair tissue process (SILVA et al, 2012B).…”

“…After each experimental period, the cytotoxicity was analyzed using 20 µl/well MTT solution (Sigma ® , St. Louis, MO, USA) at a concentration of 5 mg/mL for 4 hours (YOSHINO et al, 2013). The formazan that formed during this period was dissolved by adding 100 mL of acidified isopropanol solution with 0.04 M/l HCl to each well and shaking the culture plate for 30 minutes ).…”

“…Otherwise these products can cause high levels of inflammation, mediated by a number of factors including histamines, kinins, and neuropeptides, which can induce tissue destruction and slow the cicatricial process (YOSHINO et al, 2013). Therefore, these drugs should possess the ability to induce repair in the injured area without interfering with osteogenesis and cementogenesis (CAMARGO et al, 2009;SILVA et al, 2012A).…”

Mineral Trioxide Aggregate: A review of clinical usage and investigations of the effects of modifying particle size

“…257 MTA sealers show greater toxicity than AH Plus. 258 . In a four-week study, MTA Fillapex showed continual cytotoxicity.…”