In Vitro Cytotoxicity of Two Categories of Dental Cements

Abstract

Background and Aim: Resin cements are used widely in restorative dentistry regardless of their biocompatibility. The aim of this study was to compare the cytotoxicity of two categories of dental cements consisting of three chemically set cements (Fuji I, Fuji PLUS and Harvard) and two dual curing cements (BisCem and Duo-Link) by use of MTT assay. Methods and Materials: In this experimental study, four round-shaped samples of each specimen were placed in DMEM culture medium for 24, 48 and 72 hours. The extracts from each sample were applied on L929 mouse fibroblasts. At the end of each period, MTT assay was carried out to estimate the mitochondrial respiration. Data were analyzed by one-way analysis of variance (ANOVA) followed by Tukey's post-hoc test. The degree of cytotoxicity for each sample was determined according to the reference value of the control group. Results: Fuji I cement showed the least cytotoxicity while Harvard and BisCem cements showed the highest cytotoxic effect. The differences were not significant compared to the positive control (distilled water). Conclusion: This study showed that dental cements are capable of eliciting biological response in gingival and pulpal cells. They present a potential risk of tissue damage which depends on the cement's brand and curing modes.

Cytotoxicity of hydroxyapatite-tyrosine complex with gray titania coating on titanium alloy surface to L929 mouse fibroblasts

“…Moreover, the pH of the culture medium is also related to cell viability. Koosha et al reported that the pH of zinc phosphate cement medium decreased after 2 days to 6.6 21) , and this finding was supported by our results. In this study, although the culture medium had buffering capacity, the pH of the medium from the zinc phosphate cement group dropped from 7.8 to about 6.9 after 4 days.…”

“…Previous reports have demonstrated that zinc release can enhance cytotoxicity to L929 cells, which was related to elemental analysis results in the present study [19][20][21] . Moreover, the pH of the culture medium is also related to cell viability.…”

Effects of Aging on Compressive Strength of Two Resin-Reinforced Glass Ionomers: An In-Vitro Study

“…In previous studies, a rise in temperature was associated with a rise in the mechanical properties of composite resins; this was attributed to an increase in the rate of polymerization due to the effect of rising temperatures on initiator systems in the composite resin. (19,21) This proves that at least with regards to compressive strength, these glass ionomers do not perform as well as a composite; this, however, does not rule out the application of these materials as restorative materials for Class I restorations as more than one factor is involved in the outcome of such restorations. (5) Moreover, in a clinical situation, polymerization shrinkage will be more profound due to the consequences of the C-factor (22) .…”