Cytotoxicity of hydroxyapatite-tyrosine complex with gray titania coating on titanium alloy surface to L929 mouse fibroblasts

Abstract

Hydroxyapatite particles (HAp) have been widely used by many dental implant systems as an implant coating material because of their osteoconductive properties. This study aimed at improving the antibacterial effect of HAp as a substitute for antibiotic agents which can increase drug resistance. HAp/gray titania was selected as the coating material for on the titanium alloy substrate due to its antibacterial properties after photocatalytic reaction. When combined with amino acids, HAp can form a fluorescent complex which enhances this property. Before clinical application, this new coating should be examined for cytotoxic effects against biological cells or tissues. Therefore, L929 mouse fibroblasts were used to represent fibrous tissue surrounding dental implant. After performing a 6-day alamarBlue assay, the new coating method using hydroxyapatite-tyrosine complex with gray titania on titanium alloy surface can be said to have no influence on the growth of fibroblasts.

Ag- and Sr-enriched nanofibrous titanium phosphate phases as potential antimicrobial cement and coating for a biomedical alloy

“…[13][14][15][16] However, other nanostructured metal-containing composites that are synthesized using the substrate metal as an inorganic source are also used for this application. [17,18] A good example for these metals is titanium, because of its capacity for osseointegration and ability to physically bond with bone, thanks to the spontaneously formed oxide layer on its surface that possesses a high dielectric constant that does not cause denaturation of the proteins in the proximity of the implant site . [19] Therefore, the formation of a coating layer of a nanostructured titaniumcontaining compound on a titanium surface and its resulting applications have been positively reported, [20,21] including composites that can be used as implant materials with excellent apatiteformability.…”

Chitin Nanocrystals as an Eco-friendly and Strong Anisotropic Adhesive

“…As a promising daily-used adhesive, in vitro and in vivo biological safeties of ChNC nanoglue are critical in case of inevitable skin exposure. According to the previous researches on skin safety, we choose the mouse fibroblast cell line L929 as an in vitro model. , The cytotoxicity of ChNCs was assessed by exposing L929 mouse fibroblasts to control and gradient concentrations of ChNCs for 24 h or 48 h. After treatment, we performed cell counting kit-8 (CCK-8) assay to investigate the cell viability. As shown in Figure A, the cell viabilities of the ChNC-treated groups are more than 95%, and it has no significant difference from the control groups, which reflects virtually no cytotoxicity of ChNCs.…”

A Review on the Synthesis and Current and Prospective Applications of Zirconium and Titanium Phosphates

“…The outer layer of the device should be compromised of compounds naturally present in the biological environment, commonly calcium phosphate phases [116][117][118][119]. However, nanostructured systems are also used for this application using the metal of the (metallic) substrate as the source of the inorganic matter [120,121]. A good example of these metals is titanium, with high osseointegration capacity and ease of physical attachment to bone, owing to the spontaneously formed oxide layer on its surface, which does not cause denaturation of proteins in the proximity of the implant [122].…”