In vitro Cytotoxicity of Scopoletin Derived from Eupatorium laevigatum Lam.

Abstract

Aims: Eupatorium laevigatum Lam. is commonly used as anti-inflammatory, antiseptic, antirheumatic, and in the treatment of colds and ulcers. The present study aimed to characterize the active fractions of the aerial parts of E. laevigatum, isolate its major constituents and to evaluate its cytotoxic effects against human tumor cells. Methodology: Phytochemical analysis of the aerial parts of E. laevigatum detected the presence of flavonoids, saponins and coumarins. Nuclear magnetic resonance with carbon and hydrogen determined that coumarin to be scopoletin. The human cancer cell lines HT-29, NCI-H460, MCF-7 and RXF-393 were used to evaluate cytotoxicity through the sulforodamine B assay as well the evaluation of oxidative damage through the thiobarbituric acid reactive species assay. Short Research ArticleResults: Our study has shown that E. laevigatum crude extract and chloroform, ethyl acetate and butanol fractions are not cytotoxic in the concentrations used (up to 100 µg/mL), but the coumarin scopoletin isolated from the aerial parts of E. laevigatum presented a cytotoxic effect against NCI-H460 and RXF-393 cells (IC 50 value of 19.1 and 23.3 µg/mL, respectively). Scopoletin did not show any oxidative effect. Conclusion:The coumarin scopoletin can be found in E. laevigatum and this compound induces cytotoxicity in NCI-H460 and RXF-393 cell lines. Moreover, it is suggested that the cytotoxic effect of scopoletin is no related to oxidative damage.

Optimisation of artemisinin and scopoletin extraction from Artemisia annua with a new modern pressurised cyclic solid–liquid (PCSL) extraction technique

“…Scopoletin (6‐methoxy‐7‐hydroxycomarin) is a phenolic coumarin and an important member of the group of phytoalexins isolated from many plants . A variety of biological activities like anti‐inflammatory, anti‐allergy and anti‐angiogenesis have been reported for scopoletin, known also for its cytotoxicity towards cancer cells …”

“…43 A variety of biological activities like antiinflammatory, anti-allergy and anti-angiogenesis have been reported for scopoletin, known also for its cytotoxicity towards cancer cells. [19][20][21] In the literature, there are many different extraction techniques used to obtain artemisinin in particular from the fresh plant matrix. The methods used are generally time-consuming, needing often instruments with relatively critical purchase and operating costs, and also showing a strong environmental impact and extractive efficiency that is not always optimal ( Table 2).…”

“…A variety of other biological activities like anti‐inflammatory, anti‐allergy and anti‐angiogenesis have been ascribed to scopoletin (Figure ), a phenolic coumarin and an important phytoalexin isolated from many plants . Scopoletin is also known for its cytotoxicity towards cancer cells …”

“…17,18 Scopoletin is also known for its cytotoxicity towards cancer cells. [19][20][21] Despite numerous attempts to synthesise artemisinin in vitro, to date the only pharmacological source of this molecule remains the plant. The main commercial extraction process is based on the use of hot petroleum ether as extracting agent, sometimes modified with ethyl acetate (EtOAc) to increase solubility of artemisinin.…”

Separation Methods of Phenolic Compounds from Plant Extract as Antioxidant Agents Candidate

“…Scopoletin or 7-hydroxy-6-methoxy coumarin is an example of a phenolic coumarin that contains two aromatic rings with a hydroxyl and methoxy group, as well as an oxo group [52]. Scopoletin can be isolated from many plants such as Eupatorium laevigatum [53], the root of Hypochaeris radicata [54], and Lasianthus lucidus Blume [54].…”