In Vitro Cytotoxic Activity of Isolated Compounds From Viburnum Punctatum Buch-Ham Ex D. Don

Alex, A. Renjith; Ilango, K.

doi:10.22159/ijcpr.2017v9i1.16622

Cited by 3 publications

(3 citation statements)

References 3 publications

(3 reference statements)

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“…In many situations, the actual compound isolated from the plant may not serve as the drug but leads to the development of potential novel agents. The ability to attach agents to carrier molecules directed to specific tumors holds promise for the effective targeting of highly cytotoxic natural products to the tumors while avoiding their toxic side effects on normal healthy tissues [29].…”

Section: Resultsmentioning

confidence: 99%

The Evaluation of Antimicrobial and Cytotoxic Activity of the Essential Oil Extracted From the Aerial Parts of Southernwood Herb (Artemisia Abrotanum L.) That Recently Grown in Iraq

Almahdawy

Said

Abbas

et al. 2017

Asian J Pharm Clin Res

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Objective: This research is to study the assessment of the antimicrobial and cytotoxic activity of the essential oil extracted from the aerial parts of Artemisia abrotanum L. that recently grown in Iraq. Methods:The essential oil of A. abrotanum was extracted by hydrodistillation using Clevenger apparatus. This essential oil was tested for antimicrobial activity of five different pathogenic microorganisms (Gram-positive [Staphylococcus aureus and Bacillus subtilis] and Gram-negative [Salmonella typhi and Escherichia coli] bacterial strains) and fungi: Candida albicans using diffusion well agar method. Furthermore, this essential oil was tested for cytotoxic activity using rhabdomyosarcoma cell line, and the growth or inhibition of cancer cells was measured by MTT method. Results:The obtained results show that the antibacterial activity for A. abrotanum against S. aureus was at concentrations 40, 25, and15 µl with minimum inhibitory concentrations of 20 mm, while it showed antibacterial activity against E. coli for four different concentrations of 40, 25, 15, and10 μl with inhibition zone of 16, 12, 14, and 10 mm, respectively, and it showed antifungal activity against C. albicans at four concentrations 40, 25, 15, and10 μl with inhibition zone of 18, 24, 26, and 30 mm, respectively. The cytotoxic activity of the extracted essential oil was showed that the three concentrations of the extract (25, 50, and 100 μg/ml) were all lower significantly as compared to dimethyl sulfoxide group. A significant difference was seen for group 25 with both groups 50 and 100, but no significant difference was seen between the two later. Finally, the antimicrobial and anticancer activity of this plant could be due to its essential oil constituents: Borneol, cymene, camphor, terpineol, eucalyptol, and aromadendrene. Conclusion:The essential oil of A. abrotanum L. has a potent antimicrobial and anticancer effect against the tested microbial organisms and the cancer cells.

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Section: Resultsmentioning

confidence: 99%

The Evaluation of Antimicrobial and Cytotoxic Activity of the Essential Oil Extracted From the Aerial Parts of Southernwood Herb (Artemisia Abrotanum L.) That Recently Grown in Iraq

Almahdawy

Said

Abbas

et al. 2017

Asian J Pharm Clin Res

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“…In vitro anticancer activity of aerial parts of V. punctatum was tested using chloroform and methanol extracts and the HCT 15 cell line (human colon carcinoma). Maximal concentrations of the extracts (400 μg/ml) inhibited 63:93 ± 2:76% and 80:16 ± 2:13% of cell viability, respectively [183]. V. punctatum chloroform and methanol extracts expressed a hepatoprotective activity in vitro protecting the Chang liver cells against CCl4induced toxicity in the MTT test.…”

Section: Cytotoxic Activities Of Viburnum Plants (In Vitromentioning

confidence: 91%

Genus Viburnum: Therapeutic Potentialities and Agro‐Food‐Pharma Applications

Sharifi‐Rad

Quispe

Valdés

et al. 2021

Oxidative Medicine and Cellular Longevity

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The genus Viburnum (Adoxaceae, Dipsacales) is of scientific interest due to the chemical components and diverse biological activities found across species of the genus, which includes more than 230 species of evergreen, semievergreen, or deciduous shrubs and small trees. Although frequently used as an ornament, the Viburnum species show biological properties with health-promoting effects. Fruits, flowers, and barks of certain species are used for pharmaceutical purposes or as cooking ingredients, hence containing biochemical compounds with health-promoting activity such are carotenoids, polyphenols, and flavonoids. However, its taxonomical determination is difficult, due to its wide distribution and frequent hybridizations; therefore, an objective classification would allow us to understand its biological activity based on its phytochemical components. More than sixty phytochemical compounds have been reported, where vibsanin-type diterpenes and their derivatives are the most prevalent. Leaves and twigs of V. dilatatum contain the largest number of phytochemicals among the genus. Through preclinical evidence, this study provides insight regarding antioxidant, antibacterial, anti-inflammatory, cytotoxic, and anticancer activities of genus Viburnum.

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“…The principle involved is the cleavage of tetrazolium salt MTT into a blue colored product (formazan) by mitochondrial enzyme succinate dehydrogenase. The number of cells was found to be proportional to the extent of formazan production by the cells used [15].…”

Section: Antiproliferative Assaymentioning

confidence: 94%

Evaluation of Antiproliferative Activity of Siddha Anti-Psoriatic Formulation Panchamuga Chendhuram Using Cultured Human Keratinocyte Cell Lines

Rajalakshmi¹,

Vt²,

Samraj³

2019

Asian J Pharm Clin Res

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Objectives: This study was aimed at scientifically evaluating the in vitro antipsoriatic activity of Siddha drug Panchamuga Chendhuram (PMC) in human keratinocyte (HacaT) cell lines. Methods: The Siddha drug PMC tested for antipsoriatic activity on HacaT cell lines was morphologically examined by phase contrast microscopy, and the cell viability was determined by 3- (4, 5 dimethyl thiazole-2 yl) -2.5-diphenyl tetrazolium bromide assay. About 100 μl of different concentrations (2, 6, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 μg/ml) of the test samples were prepared in the cell culture medium and incubated for 24 h and 48 h to determine the viable cells. Results: The results revealed that Siddha drug PMC showed hopeful antiproliferative activity. In vitro studies showed that after 24 h and 48 h incubation, the inhibitory concentration 50 (IC50) values of PMC (IC50 20 μg/ml) were 72.08±27.56 μg/ml and 43.91±17.71 μg/ml, respectively, as compared with Asiaticoside as a positive control with an IC50 value of 20.13 μg/ml. Conclusion: Thus, this study provides scientific evidence about the efficacy of the Siddha drug PMC against the HacaT cell lines confirming its traditional use in psoriasis treatment and also emphasizes the need for antipsoriatic evaluation in animal models.

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In Vitro Cytotoxic Activity of Isolated Compounds From Viburnum Punctatum Buch-Ham Ex D. Don

Cited by 3 publications

References 3 publications

The Evaluation of Antimicrobial and Cytotoxic Activity of the Essential Oil Extracted From the Aerial Parts of Southernwood Herb (Artemisia Abrotanum L.) That Recently Grown in Iraq

The Evaluation of Antimicrobial and Cytotoxic Activity of the Essential Oil Extracted From the Aerial Parts of Southernwood Herb (Artemisia Abrotanum L.) That Recently Grown in Iraq

Genus Viburnum: Therapeutic Potentialities and Agro‐Food‐Pharma Applications

Evaluation of Antiproliferative Activity of Siddha Anti-Psoriatic Formulation Panchamuga Chendhuram Using Cultured Human Keratinocyte Cell Lines

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