In vitro cytotoxic activity of Thai medicinal plants used traditionally to treat cancer

Itharat, Arunporn; Houghton, Peter J.; Eno-Amooquaye, E.; Burke, Philip J.; Sampson, Julia; Raman, Amala

doi:10.1016/j.jep.2003.09.014

Cited by 217 publications

(160 citation statements)

References 9 publications

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“…Breast cancer (MCF-7) and cervical cancer were used in the present study in vitro. Cytotoxicity was done according to the method of Itharat, et al [51]. IC50 was calculated by analysis between surviving fraction and concentration [52].…”

Section: Cytotoxicity and Anticancer Activity In Vitromentioning

confidence: 99%

“…It can be observed that L-asparaginase inhibits cell proliferation of HEPG2 and HCT-116 (human cancer cell lines) that could arrest the cell cycle and generate apoptosis, which explain the in vitro anti-proliferative effect of L-asparaginase. Cancer cells exhibit rapid growth and cell division, and therefore have an increased nutritional need than normal cell [23,51]. In most human cells deficiency, L-asparagine is produced from aspartic acid and glutamine by asparagine synthetase.…”

Section: Cytotoxicity and Anticancer Activity Ofl-aspargenasementioning

confidence: 99%

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Anticancer Activity of L-Asparaginase Produced from Vigna Unguiculata

Moharib

2018

World Scientific Research

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The present study aimed to production of purified L-asparaginase from Vignaunguiculata. Different physiological parameters, such as pH, temperature and incubation period, were optimized for growth and maximum L-asparaginase production. The optimum parameters were 37°C, 30 min and pH 8.5. Maximum L-asparaginase was 886.4U/ml with a specific activity of 1140.7 U/ml (31 fold purification with 28 %yield) were obtained at optimum conditions. The purified L-asparaginase produced from Vignaunguiculata was used for characterization and general properties. The effect of pH and temperature on L-asparaginase activity as well as stability at different pH and temperature were determined. The optimum pH 8.5 and 37ºC temperature on L-asparaginase showed 100% residual activity. Stability of pH around 8.5 and temperature 70ºC showed 90 and 78 % residual activity at 30 and 60 min respectively. The Lasparaginase showed high stability at alkaline pH (pH 8.5) when incubated for up to 60h.The molecular weight of the produced L-asparaginase was close to 68.5 kDa. Cytotoxic activity of Lasparaginase was examined in vitro using four carcinoma cell lines. L-aspargenase has higher effective in growth inhibition against HEPG2 and HCT-116 but lower against HELLA and MCF7 carcinoma cell lines. The data show that L-aspargenase has a higher cytotoxic activity against HEPG2 and HCT116, revealed higher percentage of cell death, indicating antitumor properties, and demonstrate direct effect on cancer cell proliferation of HEPG2 and HCT116. Therefore, Vignaunguiculata was considered to be a suitable source for production of Lasparaginase has higher activity and good stability. Purified L-asparaginase obtained from Vignaunguiculata could be employed in drug chemotherapy and treatment of cancer.

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Section: Cytotoxicity and Anticancer Activity In Vitromentioning

confidence: 99%

Section: Cytotoxicity and Anticancer Activity Ofl-aspargenasementioning

confidence: 99%

Anticancer Activity of L-Asparaginase Produced from Vigna Unguiculata

Moharib

2018

World Scientific Research

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“…Conforme os critérios estabelecidos pelo Instituto Nacional Americano de Câncer (INC), a citotoxicidade de extratos brutos em ensaios preliminares deve apresentar o limite máximo de IC 50 de 30 µg/mL para ser considerado promissor para purificação e emprego como antineoplásico (Suffness & Pezzuto, 1990;Itharata et al, 2004;Alzeer et al, 2014). Desta forma, os extratos clorofórmico e hexânico de E. anomala apresentaram citotoxicidade muito interessante e promissora, pois o IC 50 encontrado para ambos (25,56 e 31,25 µg/mL, respectivamente) esteve próximo ao estabelecido para o emprego como antineoplásico.…”

Section: Citotoxicidade In Vitro Dos Extratosunclassified

Avaliação da atividade antimicrobiana de extratos de Eugenia anomala e Psidium salutare (Myrtaceae) frente à Escherichia coli e Listeria monocytogenes

Simonetti

Ethur

Castro

et al. 2016

Rev. bras. plantas med.

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RESUMO:As doenças transmitidas por alimentos ocorrem principalmente devido à ingestão de alimentos contaminados por microrganismos patogênicos, dentre eles a Escherichia coli e Listeria monocytogenes. Uma das alternativas estudadas para minimizar a contaminação de alimentos é o emprego de plantas, ou seus extratos, como agentes antimicrobianos de origem natural em produtos alimentícios. Desta forma o objetivo do presente estudo é fornecer dados científicos a respeito de duas plantas nativas do RS ainda não estudadas, Eugenia anomala e Psidium salutare, visando potencial emprego como agente antimicrobiano natural em alimentos. Para tanto, avaliou-se a atividade antimicrobiana de extratos de E. anomala e P. salutare contra E. coli e L. monocytogenes através da determinação da concentração inibitória mínima (CIM) pelo método de microdiluição em caldo, a capacidade antioxidante dos extratos por meio do método de redução do radical DPPH e a citotoxicidade in vitro empregando células CHO-K1. Os resultados obtidos mostraram que os extratos de acetato de etila e etanólico de ambas as espécies possuem ação antioxidante muito alta, de 94,08% e 93,86%, respectivamente. Apenas o extrato hexânico de P. salutare apresentou ação antimicrobiana moderada (CIM = 312,5 µg/mL). Todos os extratos apresentaram ação citotóxica sendo que os maiores percentuais foram do extrato clorofórmico de E. anomala (77,05%) e hexânico de P. salutare (76,79%), na concentração de 100 µg/mL. Assim, o presente estudo demonstrou que as espécies vegetais estudadas apresentam potencial para emprego como agente antimicrobiano destes microrganismos.Palavras-chave: Concentração Inibitória Mínima, Citotoxicidade in vitro, Atividade Antioxidante.ABSTRACT: Evaluation of the antimicrobial activity of extracts of Eugenia anomala and Psidium salutare (Myrtaceae) against the Escherichia coli and Listeria monocytogenes. The foodborne diseases occur mainly due to the ingestion of food contaminated by pathogenic microorganisms, including Escherichia coli and Listeria monocytogenes. One of the alternatives studied to minimize contamination of food is the use of plants or their extracts as antimicrobial agents naturally occurring in food products. The objective of this study is to provide scientific data on two native plants of RS have not studied Eugenia anomala and Psidium salutare for a potential use as a natural antimicrobial agent in food. To this end, we evaluated the antimicrobial activity of extracts of E. anomala and P. salutare against E. coli and L. monocytogenes by determining the minimum inhibitory concentration (MIC) by the broth microdilution method, the antioxidant capacity of the extract for means DPPH radical reduction method and in vitro cytotoxicity using CHO-K1 cells. The results showed that the ethyl acetate and ethanolic extracts of both species have very high antioxidant activity, of 94.08% and 93.86%, respectively. Only the hexane extract of P. salutare showed a moderate antimicrobial activity (MIC = 312.5 mg/mL). Moreover, all extracts showed ...

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“…[7] In fact, the medicinal plants are easily available, cheaper and possess less toxicity as compared to the modern allopathic drugs. [8,9] Phytoconstituents promotes and strengthens digestion. It also rejuvenates the body tissues & controls the metabolic activities of the body.…”

Section: World Journal Of Pharmacy and Pharmaceutical Sciencesmentioning

confidence: 99%