IN VITRO CULTURE OF HUNGARIAN APRICOT (Prunus armeniaca L.) VARIETIES

Balla, I.; Vértesy, J.

doi:10.17660/actahortic.2001.560.75

Cited by 5 publications

(4 citation statements)

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“…The number of shoots were significantly affected by the system of subculture and it was higher in the case of using a propagation medium only (Table 6 and Figure 5), confirming Balla and Vertesy (2001) working with Hungarian apricot cultivars. The use of an elongation medium had no effect on shoot length and is therefore not recommended during the proliferation stage.…”

Section: System Of Subculturementioning

confidence: 52%

“…(c) One min immersion in 70% ethanol and then 15 min immersion, with agitation, in 0.75% NaOCl containing three drops of Tween 20/ 500 ml. Finally stems were rinsed thrice with sterile deionized water and cultured on Balla propagation medium (Balla and Vertesy, 2001), supplemented with 4.4 lM BA, 0.57 lM IAA, 3% (w/v) sucrose and 0.6% (w/v) agar (B&V S.r.L., type S 1000).…”

Section: Plant Materials and Culture Conditionsmentioning

confidence: 99%

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Improvement of in vitro propagation of apricot cultivar ‘Bebecou’

Koubouris

Vasilakakis

2006

Plant Cell Tiss Organ Cult

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Factors affecting successful establishment in vitro, rapid proliferation and rooting of apricot cultivar 'Bebecou' were studied. Ethanol and NaOCl were applied in several combinations for disinfection; chilling, plant growth regulators BA, IAA and GA 3 , antibiotics, different culture vessels and systems of subculture were evaluated for the optimization of shoot proliferation and the auxins NAA and IBA were assessed for root induction. The highest number of new microshoots/explant (18.7) was obtained in a culture medium supplemented with 2.2 lM BA+0.57 lM IAA after 300 h of chilling. The effect of GA 3 (11.4 lM) on shoot proliferation was positive in combination with 4.4 or 8.9 lM BA. Shoot length and productivity were highest at 2.2 lM BA+11.4 lM GA 3 +0.57 lM IAA and at 2.2 lM BA+0.57 lM IAA, respectively and decreased as cytokinin concentration increased. The antibiotic 'Na-cefotaxime' had a minimal impact on shoot growth when used at the lowest concentration (250 mg l )1 ). Subculture every 2 weeks in a medium supplemented with 2.2 lM BA and 0.57 lM IAA was more efficient for shoot induction than alternation of 20 days culture in a propagation medium supplemented with 2.2 lM BA and 10 days culture in an elongation medium supplemented with 1.

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Section: System Of Subculturementioning

confidence: 52%

Section: Plant Materials and Culture Conditionsmentioning

confidence: 99%

Improvement of in vitro propagation of apricot cultivar ‘Bebecou’

Koubouris

Vasilakakis

2006

Plant Cell Tiss Organ Cult

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“…Thus, it is important to establish effective protocols for micropropagation of apricot with explants taken from mature trees, as micropropagation offers a possible solution to the problems of clonally propagated elite apricot varieties. Numerous studies on the micropropagation of the apricot have been carried out using the juvenile or adolescent seedlings of mature trees (Skirvin et al 1979;Snir 1984;Mante et al 1989;Schmidt and Ketzel 1996;Murai et al 1997;Kramarenko 1999;Perez-Tornero et al 1999, 2000a, 2000bPerez-Tornero and Burgos 2000;Hokanson and Pooler 2000;Balla and Vertesy 2001;Gentile et al 2002;Burgos and Alburquerque 2003;Paris et al 2004;Petri et al 2005;Srinivasan et al 2005;Yıldırım 2006;Koubouris and Vasilikakis 2006). Unfortunately, the results of this research are difficult to replicate because the optimal conditions for propagation are genotype dependent (Perez-Tornero and Burgos 2000).…”

Section: Introductionmentioning

confidence: 99%

Pyrazinamide monoresistance in clinical isolates

Kalir¹,

Özkütük²,

Esen³

et al. 2013

Turkish Journal of Medical Sciences

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“…In vitro thermotherapy was carried out at 35-37°C for 20 days, following a gradual temperature increase (1°C per day) for explants acclimatization. Balla elongation medium (Balla and Vertesy 2001) supplemented with 1.1 lM benzyladenine (BA), 5.71 lM indole-3-acetic acid (IAA), 3% (w/v) sucrose and 0.6% (w/v) agar (Plantagar S.1000, B&V The Agar Co., Gattatico RE, Italy), was used. Subsequently, 5 mm long shoot tips were excised and transferred to fresh Balla propagation medium supplemented with 2.2 lM BA, 0.57 lM IAA, 3% (w/v) sucrose and 0.6% (w/v) agar.…”

mentioning

confidence: 99%

Elimination of Plum pox virus through in vitro thermotherapy and shoot tip culture compared to conventional heat treatment in apricot cultivar Bebecou

et al. 2007

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The effectiveness of in vitro thermotherapy (explants at 35-37°C for 20 days) in obtaining propagating material of apricot (Prunus armeniaca L.) cultivar Bebecou that is free of Plum pox virus (PPV) was compared to a conventional heat treatment technique (potted plants at 30-35°C for 8 weeks). An improved protocol for in vitro thermotherapy was combined with shoot tip culture. The protocol is 5.8 times more effective than conventional thermotherapy while taking half the time and is likely to have a high commercial impact for nurseries.

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IN VITRO CULTURE OF HUNGARIAN APRICOT (Prunus armeniaca L.) VARIETIES

Cited by 5 publications

References 0 publications

Improvement of in vitro propagation of apricot cultivar ‘Bebecou’

Improvement of in vitro propagation of apricot cultivar ‘Bebecou’

Pyrazinamide monoresistance in clinical isolates

Elimination of Plum pox virus through in vitro thermotherapy and shoot tip culture compared to conventional heat treatment in apricot cultivar Bebecou

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