In an attempt to determine the relationship between the features of houses and moulds growing indoors, 242 houses were examined in Izmir, a city on the west coast of Turkey with a mild climate. During house visits a questionnaire was given and air was sampled using an 'air IDEAL' air sampler for quantitative fungal culture. The moulds most commonly isolated were Aspergillus, Penicillium, and Mucor spp. Aspergillus growth in houses older than 20 years was more common than other species when the features of houses and isolated fungi were compared. Mucor grew significantly more in houses where the air was humid, the temperature was cooler and there were pot plants. Penicillium grew more frequently in houses where visible mould was present and birds were bred. However, no relationship could be demonstrated between the method of heating, the number of household members, exposure to sun, type of building and flooring with a specific mould. As a conclusion, many household factors suggested as risk factors for mould growth have been examined and only a few relationships could be established between certain features of houses and moulds. However, mould growth is much affected by many conditions and the environment in a house is one of many factors that may facilitate growth.
The typing of non-tuberculous mycobacteria (NTM) is important from a clinical and epidemiological perspective. The polymerase chain reaction-restriction enzyme analysis (PRA) method and DNA sequence analysis method were utilized to target a gene region that codes the 65-kDa heat-shock protein for typing 150 suspected NTM samples isolated from the respiratory tract. Mycobacterium abscessus, Mycobacterium xenopi, Mycobacterium fortuitum, and Mycobacterium peregrinum were most frequently found by both methods. Six isolates that could not be defined by the PRA method were defined as Nocardia cyriacigeorgica, Nocardia abscessus, and Mycobacterium intracellulare by DNA sequence analysis. Discordance between the results of the two methods was observed for only one isolate. The isolate that was defined as Mycobacterium gordonae type 6 by the PRA method was defined as Mycobacterium senegalense by sequence analysis. The PRA method is simple and gives rapid results. Compared with DNA sequence analysis, it gives consistent and reliable results up to a ratio of 90%. DNA sequence analysis is the gold standard method in which all strains can be defined. However, given our laboratory conditions, its disadvantage is that it takes longer to reach a diagnosis than through the PRA method.
Tüberküloz dışı mikobakteriler (TDM) çevrede yaygın olarak bulunur ve özellikle immün sistemi baskılanmış insanlarda etken olarak izole edilmektedir. Klasik tüberküloz ilaçlarına büyük oranda dirençli olmaları ve giderek artan sıklıkta izole edilmeleri nedeniyle TDM enfeksiyonlarının doğru ve erken tanısı tedavi başarısı açısından çok önemlidir. Bu çalışmada, Dokuz Eylül Üniversitesi Hastanesi'nde 2012-2016 yılları arasında Mikobakteriyoloji Laboratuvarı'nda çalışılmış ve TDM saptanmış örneklerden elde edilen sonuçlar retrospektif olarak değerlendirilmiş ve yıllar içindeki değişim incelenmiştir.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.