In vitro culture and micropropagation of the Baetic-Moroccan endemic plant Lapiedra martinezii Lag. (Amaryllidaceae)

Ornamental potential of the coastal plant Lapiedra martinezii Lag. (Amaryllidaceae): the role of its revalorization in xero-gardening and ex-situ conservation

“…Callus induction was satisfactory using several plant growth regulators. However, none of the callus produced was able to be regenerated into new plants [63].…”

“…As a result of the quick seedling establishment, bulbils rapidly appear from young plantlets thus constituting a suitable material for in vitro initiation, swelling and ex vitro acclimatization [84]. In vitro propagation from adult bulbs was also achieved and a yield of approximately 6 bulbils per explant were obtained using solid cultures supplemented with plant growth regulators after 8 weeks of cultivation [63]. In addition, increasing the sucrose concentration up to 90 g/L in the culture medium almost doubled the biomass of bulbils produced in vitro [63].…”

“…In vitro propagation from adult bulbs was also achieved and a yield of approximately 6 bulbils per explant were obtained using solid cultures supplemented with plant growth regulators after 8 weeks of cultivation [63]. In addition, increasing the sucrose concentration up to 90 g/L in the culture medium almost doubled the biomass of bulbils produced in vitro [63]. However, the introduction under permanent and temporary immersion systems did not improve the results obtained on solid media in any case [84].…”

“…In addition, these techniques are a prerequisite in most tools in biotechnology, especially in the context of breeding programs for transformation, improvement and new cultivar selection [61,62]. Some studies have also been made using in vitro techniques for L. martinezii propagation with successful yields [27,63,84]. An overview of the main achievements is shown in Table 1.…”

Micropropagación del lirio amazónico (Eucharis grandiflora Planch. & Linden) mediante organogénesis directa

“…Una alternativa es la micropropagación, técnica que ha sido probada exitosamente en la propagación masiva de varias especies vegetales que presentan limitaciones importantes (Anis & Ahmad, 2016;Cardoso, Sheng Gerald, & Teixeira da Silva, 2018). En especies de la familia Amaryllidaceae, el uso de esta estrategia se encuentra ampliamente documentado (El Tahchy et al, 2011;Juan-Vicedo, Pavlov, Ríos, & Casas, 2019;Rahimi Khonakdari, Rezadoost, Heydari, & Mirjalili, 2020;Zayed, El-Shamy, Berkov, Bastida, & Codina, 2011), aunque en lirio amazónico los reportes son escasos y se han centrado en el estudio de algunos factores que intervienen durante la regeneración de brotes adventicios, como el tamaño y la orientación de los explantes (escamas dobles y pedicelos), la concentración de las sales inorgánicas del medio de cultivo, la temperatura y el fotoperiodo (Hasegawa, Takigawa, & Tokuzumi, 1995;Pierik, Sprenkels, & van Bragt, 1983), así como también aquellos que participan durante la embriogénesis somática (Mujib, Banerjee, & Ghosh, 2005;Mujib, Banerjee, Maqsood, & Ghosth, 2013). El objetivo de esta investigación fue elaborar una metodología que permita la micropropagación de esta especie mediante organogénesis directa con el fin de incrementar las tasas de propagación y obtener material homogéneo.…”