In vitro Culture and Biochemical Attributes of Bergenia ciliata (Haw.) Sternb.

Rafi, Sabreena; Kamili, Azra N.; Ganai, Bashir Ahmad; Mir, Mohammad Yaseen; Parray, Javid A.

doi:10.1007/s40011-016-0797-9

Cited by 13 publications

(7 citation statements)

References 16 publications

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“… Maraffa et al . (1980) and Rafi et al . (2016) similarly reported that no callus formation was observed in the explants of H. carnosa and Bergenia ciliata (Saxifragaceae) that were cultured in MS medium incorporated with 2,4-D or IBA alone, respectively.…”

Section: Discussionmentioning

confidence: 96%

Vegetative Propagation of Hoya imperialis and Hoya coronaria by Stem Cutting and Micropropagation

Don

Hamid

Taha

et al. 2021

TLSR

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Hoya imperialis (H. imperialis) and H. coronaria (Apocynaceae) are known to have ornamental value due to their beautiful flowers; however, the feasibility of propagating these plants have not been reported despite the wild populations in Brunei Darussalam being highly threatened due to habitat loss and overcollection. Thus, the present study aimed to conduct a preliminary study of the feasibility of two alternative propagation methods, stem cutting and micropropagation, as a potential approach for their ex situ conservation. Hoya stem cuttings were treated with either indole-3-butyric acid (IBA) or 1-naphthaleneacetic acid (NAA) (0–2000 mg/L), and then propagated onto a mixture of peat moss and perlite. For micropropagation, Hoya leaf explants were cultured onto Murashige and Skoog (MS) agar media that were supplemented with IBA and/or kinetin (KN) (0–10.0 mg/L). This present study shows that both Hoya species were successfully propagated by stem cutting even without hormone treatment. However, interestingly, in H. imperialis, when compared with control, the mean number of new leaves (6.3 ± 1.0) and the mean relative growth rate (RGR) based on stem diameter (0.004 ± 0.0007 cm cm−1 day−1) significantly increased when treated with 500 mg/L NAA and 2000 mg/L IBA, respectively. Meanwhile, in H. coronaria, significantly higher mean number of roots was achieved by treating with 1000 mg/L NAA (16.6 ± 1.4) or 2000 mg/L IBA (17.5 ± 2.7) compared with control. For micropropagation, callus induction was not promising and could only be observed at specific concentrations of both IBA and KN, with H. imperialis appearing to be more responsive towards these hormones in comparison to H. coronaria. The present study showed that stem cutting appeared more feasible in propagating both Hoya species.

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Section: Discussionmentioning

confidence: 96%

Vegetative Propagation of Hoya imperialis and Hoya coronaria by Stem Cutting and Micropropagation

Don

Hamid

Taha

et al. 2021

TLSR

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“…Sterilization of explants is necessary before exposing it for in vitro propagation as it confirms the decrease in contamination rate and an increase in the survival rate of plantlets. In present study, 100% sterilization and 100% survival rate was obtained at 0.02% (w/v) HgCl 2 for [29], Cichorium inlybus [30], Catharanthus roseus [31]. Many researchers have used HgCl 2 for surface sterilization [32,33] under in vitro conditions.…”

Section: Plos Onementioning

confidence: 96%

Promoting the accumulation of scopolamine and hyoscyamine in Hyoscyamus niger L. through EMS based mutagenesis

et al. 2020

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The overexploitation of medicinal plants is depleting gene pool at an alarming rate. In this scenario inducing the genetic variability through targeted mutations could be beneficial in generating varieties with increased content of active compounds. The present study aimed to develop a reproducible protocol for in vitro multiplication and mutagenesis of Hyoscyamus niger targeting putrescine N-methyltransferase (PMT) and 6β-hydroxy hyoscyamine (H6H) genes of alkaloid biosynthetic pathway. In vitro raised callus were treated with different concentrations (0.01%-0.1%) of Ethyl Methane Sulfonate (EMS). Emerging multiple shoots and roots were obtained on the MS media supplemented with cytokinins and auxins. Significant effects on morphological characteristics were observed following exposure to different concentrations of EMS. EMS at a concentration of 0.03% was seen to be effective in enhancing the average shoot and root number from 14.5±0.30 to 22.2 ±0.77 and 7.2±0.12 to 8.8±0.72, respectively. The lethal dose (LD 50) dose was calculated at 0.08% EMS. The results depicted that EMS has an intense effect on PMT and H6H gene expression and metabolite accumulation. The transcripts of PMT and H6H were significantly upregulated at 0.03-0.05% EMS compared to control. EMS treated explants showed increased accumulation of scopolamine (0.639 μg/g) and hyoscyamine (0.0344μg/g) compared to untreated.

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“…The assessment of photosynthetic pigments is an important indicator to determine the physiological state of the plant [49], i.e. the quantity and quality of chlorophyll determines photosynthetic activity, thus affecting plant growth and development [50].…”

Section: Acclimatization Phasementioning

confidence: 99%

Optimizing factors influencing micropropagation of ‘Bluecrop’ and ‘Biloxi’ blueberries and evaluation of their morpho-physiological characteristics during ex vitro acclimatization

Tejada-Alvarado

Meléndez-Mori

Valqui

et al. 2022

JBR

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BACKGROUND: Blueberry production has generated great commercial expectations, therefore for its agricultural expansion it is necessary to overcome the challenges at the time of mass propagation. OBJECTIVE: Evaluate the effect of a set of factors influencing micropropagation, as well as the influence of substrates on the ex vitro morpho-physiological performance of blueberry seedlings. METHODS: A set of protocols were developed to optimize all stages of micropropagation (aseptic establishment, multiplication, rooting, and acclimatization) of blueberries. RESULTS: Explants immersed in 1.5% NaClO for 8 min and then in 0.1% HgCl2 for 2 min achieved 100% sterility and a viability rate of 86.67% for ‘Biloxi’ and 93.33% for ‘Bluecrop’. At the multiplication stage, the maximum number of shoots of ‘Biloxi’ (3.53) and ‘Bluecrop’ (2.27) were obtained on the medium supplemented with 0.2 and 10 mg L–1 silver nanoparticles (AgNPs), respectively. The percentage of in vitro rooting was significantly improved on media containing activated charcoal, with levels between 80% and 100% . In the acclimatization phase, plants grown in a substrate composed of peat and cocomix® (2:1 ratio) showed greater uniformity and better morpho-physiological behavior. CONCLUSIONS: The present results could be successfully used for large-scale commercial production of blueberries of the varieties ‘Biloxi’ and ‘Bluecrop’.

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In vitro Culture and Biochemical Attributes of Bergenia ciliata (Haw.) Sternb.

Cited by 13 publications

References 16 publications

Vegetative Propagation of Hoya imperialis and Hoya coronaria by Stem Cutting and Micropropagation

Vegetative Propagation of Hoya imperialis and Hoya coronaria by Stem Cutting and Micropropagation

Promoting the accumulation of scopolamine and hyoscyamine in Hyoscyamus niger L. through EMS based mutagenesis

Optimizing factors influencing micropropagation of ‘Bluecrop’ and ‘Biloxi’ blueberries and evaluation of their morpho-physiological characteristics during ex vitro acclimatization

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