The present work has been carried out with the objective to evaluate the morpho-biochemical changes in the EMS generated mutants of Bergenia ciliata before transferring them to field conditions. In this regard, range of EMS doses from 0.1% to 0.9% have been given. The present doses have shown adverse effect on seed germination, root number and decreased soluble protein content of plant. However shoot length, root length, chlorophyll content and total carbohydrate content were improved at lower doses (0.3%) of EMS treatment and decreased thereafter.
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