In Vitro Cultivation, Characterization and Osteogenic Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth on 3D Printed Polylactic Acid Scaffolds

İslam, Aylin; Mammadov, Emil; Kendirci, Remziye; Aytac, Ersin; Çetiner, Sedat; Vatansever, Hafize Seda

doi:10.5812/ircmj.55593

Cited by 7 publications

(3 citation statements)

References 33 publications

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“…Vaporized hydrogen peroxide sterilization (low temperature plasma sterilization) of scaffolds was performed in Near East University Hospital, Sterilization Unit. Scaffolds with a solution of hydrogen peroxide, were kept for 30 min in a solution of peroxide at room temperature, then the peroxide was removed, and scaffolds were washed 3 times with a phosphate buffer solution (pH 7.4) (20).…”

Section: Methodsmentioning

confidence: 99%

“…Animals were sacrificed 4 months after the in vivo implantation of PLA scaffolds with or without cells. For light microscopic analyses, biomaterials with or without differentiated cells were incubated for 14 days in borax-sodium carbonate buffer (0.01 M Na2CO3 and 0.3 mm Na2B4O7, pH:11) to achieve softening of PLA (20). All samples were embedded in paraffin and 5µ sections were taken.…”

Section: Culture Characterization and In Vivo Transplantation Of Diff...mentioning

confidence: 99%

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3D Baskılı PLA-İskelelerin Üzerinde Farklılaştırılmış Mezenkimal Kök Hücrelerin In Vitro ve In Vivo Uyumları

VATANSEVER¹,

Kurt²,

KENDİRCİ³

et al. 2022

JOHSE

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Aim: 3D printing made a rapid entry as a means of production of biocompatible and biodegradable materials for tissue engineering applications. 3D printing made it possible to create custom biodegradable implants and polylactic acid is one of the most promising polymers. In this study, we aimed to form both bone and cartilage differentiated from bone marrow stromal mesenchymal stem cells on 3D printed polylactic acid polymers.Materials and Methods: The polylactic acid scaffolds were designed, 3D printed and sterilized in dedicated university facilities. Mesenchymal stem cells were collected from rat bone marrow and were then differentiated to either osteoblasts or chondroblasts. The characterization of cells was analyzed using Alizarin Red, Alcian blue staining and osteonectin and collagen II by indirect immunocytochemistry. Differentiated cells were seeded on the 3D scaffold, cultured for 2 weeks. For in vivo tests, 3D scaffolds with or without differentiated bone marrow stromal mesenchymal stem cells were implanted into subcutaneous connective tissue. After the four-month implantation, the rats were sacrificed, and all samples were histochemically and immunohistochemically analyzed.Results: Osteogenic and chondrogenic differentiation from bone marrow stromal mesenchymal stem cells were performed after 2 weeks culture condition. They were positively stained both histochemically and immunohistochemically. After transfer of the cells onto 3D polylactic acid scaffold, their differentiation continued and both bone and cartilage formation were observed after histochemical and immunohistochemical analyses both under in vitro and in vivo conditions. Conclusion: 3D printed polylactic acid scaffolds supported both bone and cartilage formation, therefore, it may be conveniently used for experimental cell in vivo studies.

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Section: Methodsmentioning

confidence: 99%

Section: Culture Characterization and In Vivo Transplantation Of Diff...mentioning

confidence: 99%

3D Baskılı PLA-İskelelerin Üzerinde Farklılaştırılmış Mezenkimal Kök Hücrelerin In Vitro ve In Vivo Uyumları

VATANSEVER¹,

Kurt²,

KENDİRCİ³

et al. 2022

JOHSE

Self Cite

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“…The isolated and characterised SHEDs in the second passage (P2) were cryopreserved with slow freezing rate protocol for two years. In this protocol, SHEDs were submitted to a freezing medium consisting fetal bovine serum (FBS) and 10% dimethyl sulfoxide (DMSO) (stored at −80 °C) [10].…”

Section: Cryopreservation Protocolmentioning

confidence: 99%

Long-term effect of low-level diode laser irradiation on proliferation of stem cells from human exfoliated deciduous teeth after cryopreservation protocol

Tunç¹,

İslam²,

Çetiner³

2019

Laser Phys.

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Low-level laser irradiation (LLLI) could be useful for the stimulation of stem-cell proliferation. LLLI has important physiological properties which increase the mitochondrial activity of cells. This study aimed to evaluate the biological effect of LLLI on cryopreserved stem cell from human exfoliated deciduous teeth (SHEDs). Cryopreserved and characterised SHEDs from a previous study were used for this study. The SHEDs were exposed to GaAlAs diode laser with energy densities of 1.2 (Group 2) and 2 J cm −2 (Group 3). For the control group (Group 1), SHEDs were cultured in culture medium including Dulbecco's modified Eagle's Medium supplemented with 15% foetal bovine serum, 1% L-glutamine, 1% penicillin-streptomycin, 1% gentamycin, and amphotericin-B. The cells in the experimental groups were irradiated as previously indicated, and after the irradiation were incubated for 0, 24, 48, and 72 h at 37 °C in 5% CO 2 . Proliferation of SHEDs was analysed by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay at incubation periods of 0, 24, 48, and 72 h after the single-laser irradiation. The effects of laser irradiation on apoptosis of SHEDs at the last incubation period (72 h) were detected with the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labelling (TUNEL) method. Group 2 showed increased TUNEL-positive cells compared to Groups 1 and 3, but no significant differences between the three groups were observed (p > 0.05). Comparing Groups 2 and 3 to Group 1, the proliferation of SHEDs significantly increased after 24, 48, and 72 h (p < 0.05), but no significant differences were observed between the groups at 0 h (p > 0.05). In addition to this, at 72 h, Group 3 showed significantly higher proliferative effect on SHEDs compared to Group 2 (p > 0.05). In terms of clinical usage of long-term cryopreserved SHEDs, we can recommend to clinicians and researchers that prior to their use, LLLI may be used to increase the proliferation rate.

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3D Printing in Dentistry

Hosseini

Ebrahimi

Shamekhi

et al. 2019

Applications of Biomedical Engineering in Dentistry

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In Vitro Cultivation, Characterization and Osteogenic Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth on 3D Printed Polylactic Acid Scaffolds

Abstract: Background: Tissue engineering mainly focuses on creating appropriate conditions for the regeneration of tissues. Scaffolds, signal molecules, and stem cells interact with each other and compose the essential components of this field.

Cited by 7 publications

References 33 publications

3D Baskılı PLA-İskelelerin Üzerinde Farklılaştırılmış Mezenkimal Kök Hücrelerin In Vitro ve In Vivo Uyumları

3D Baskılı PLA-İskelelerin Üzerinde Farklılaştırılmış Mezenkimal Kök Hücrelerin In Vitro ve In Vivo Uyumları

Long-term effect of low-level diode laser irradiation on proliferation of stem cells from human exfoliated deciduous teeth after cryopreservation protocol

3D Printing in Dentistry

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