In vitro crystallisation systems for the study of urinary stone formation

Achilles, W.

doi:10.1007/bf01367662

Cited by 18 publications

(7 citation statements)

References 40 publications

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“…The weak band at 883 cm −1 corresponds to stretching vibration of CO 3 2− ion which is situated in monovalent anionic sites of the apatite structure (Fig. 3) [21,22,17]. The weak bands at 1034 and 1093 cm −1 may be assigned to C-O stretching vibrations, while one of strongest band in the spectrum at 1620 cm −1 assigned to OH bending vibrations (Table 3).…”

Section: Resultsmentioning

confidence: 94%

Characterization and FTIR spectral studies of human urinary stones from Southern India

Wilson

Bushiri

Vaidyan

2010

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Urinary stones resected from urinary bladders of patients hailing from Kollam district of Kerala State, India were analyzed by SEM, XRD and by thermal analysis techniques. The analytical results indicate that, stones have different composition, i.e., calcium phosphate, calcium phosphate hydroxide and sodium calcium carbonate. Infrared spectral studies also reveal the presence of phosphates or carbonates in these samples. Further, IR spectral investigations have revealed that amorphous carbonated species are occupied in PO(4) sites in calcium phosphate type stone and OH sites in calcium phosphate hydroxide sample. Thermal studies of these samples also reveal that, carbon dioxide is released from carbonated samples upon heating which is related to amount of carbon content and bond strength. Crystals with defects and irregular morphology are grown inside the urinary bladder due to variation in crystal growth conditions.

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Section: Resultsmentioning

confidence: 94%

Characterization and FTIR spectral studies of human urinary stones from Southern India

Wilson

Bushiri

Vaidyan

2010

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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“…We used a nonseeded system when crystallization was induced by fixed supersaturation to measure the effect of THP and UM on CaOx crystallization (17). Briefly, to a 96-wells microtiter ELISA plate we added 250 µl of a solution containing 4 mmol of calcium chloride and 150 mmol of sodium chloride in 20 mmol of sodium acetate buffer, pH 5.7.…”

Section: Crystallization Assaymentioning

confidence: 99%

Role of Tamm-Horsfall protein and uromodulin in calcium oxalate crystallization

Carvalho

Mulinari

Nakagawa

2002

Braz J Med Biol Res

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One of the defenses against nephrolithiasis is provided by macromolecules that modulate the nucleation, growth, aggregation and retention of crystals in the kidneys. The aim of the present study was to determine the behavior of two of these proteins, Tamm-Horsfall and uromodulin, in calcium oxalate crystallization in vitro. We studied a group of 10 male stone formers who had formed at least one kidney stone composed of calcium oxalate. They were classified as having idiopathic nephrolithiasis and had no well-known metabolic risk factors involved in kidney stone pathogenesis. Ten normal men were used as controls, as was a group consisting of five normal women and another consisting of five pregnant women. Crystallization was induced by a fixed supersaturation of calcium oxalate and measured with a Coulter Counter. All findings were confirmed by light and scanning electron microscopy. The number of particulate material deposited from patients with Tamm-Horsfall protein was higher than that of the controls (P<0.001). However, Tamm-Horsfall protein decreased the particle diameter of the stone formers when analyzed by the mode of the volume distribution curve (P<0.002) (5.64 ± 0.55 µm compared to 11.41 ± 0.48 µm of uromodulin; 15.94 ± 3.93 µm and 12.45 ± 0.97 µm of normal men Tamm-Horsfall protein and uromodulin, respectively; 8.17 ± 1.57 µm and 9.82 ± 0.95 µm of normal women Tamm-Horsfall protein and uromodulin, respectively; 12.17 ± 1.41 µm and 12.99 ± 0.51 µm of pregnant Tamm-Horsfall protein and uromodulin, respectively). Uromodulin produced fewer particles than Tamm-Horsfall protein in all groups. Nonetheless, the total volume of the crystals produced by uromodulin was higher than that produced by Tamm-Horsfall protein. Our results indicate a different effect of Tamm-Horsfall protein and uromodulin. This dual behavior suggests different functions. Tamm-Horsfall protein may act on nucleation and inhibit crystal aggregation, while uromodulin may promote aggregation of calcium oxalate crystals.

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“…In diesen wird das Problem einer nur unvollständigen Urinanalyse dadurch umgangen, dass in der zu untersuchenden Urinprobe eine Kristallisation künstlich ausgelöst wird [9,10,11,12]. Der Zeitpunkt des Kristallisationsbeginns sowie die Wachstums-und Aggregationsraten der gebildeten Partikel sind wichtige Parameter, welche aus den Kristallisationsexperimenten abgeleitet werden können.…”

Section: In-vitro-kristallisationsexperimenteunclassified

Überwachung des Behandlungserfolgs der bei Kalziumoxalatsteinmetaphylaxe mit dem Bonn-Risk-Index

2003

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Cost-effective metaphylaxis and monitoring is becoming more and more focused in the treatment of urinary stones. Therefore, medical practitioners are asked to reduce the analytical efforts necessary for evaluation and to control the actual biochemical risk of stone formation in the patients. The most common strategy available is based on chemical urinalysis and the calculation of theoretically derived risk parameters. However, this covers--in the best case--the analysis of the most prominent low molecular urinary constituents. No information about the fraction of the important macromolecular urinary components is obtained. Crystallization experiments in unprepared, native urine samples, carried out according to the Bonn-Risk-Index approach (BRI), allow the determination of a more realistic measure of a urine's crystallization risk since the entire urinary composition influences the experimental result. As only two parameters have to be analyzed, the BRI is a fast and cost-effective risk evaluation method. The results show a high selectivity between stone-formers and non-stone-forming persons. The changes in the BRI-risk of three calcium oxalate stone-formers after a 1 week stay in our hospital are presented and discussed in detail. In one of these patients, a follow up examination was performed in order to control the therapy's success and, additionally, to obtain information about the patient's compliance with the therapy. During hospitalization, the patients received a standardized and "stone-neutral" diet. All persons showed a distinct decrease in their individual crystallization risk.

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In vitro crystallisation systems for the study of urinary stone formation

Cited by 18 publications

References 40 publications

Characterization and FTIR spectral studies of human urinary stones from Southern India

Characterization and FTIR spectral studies of human urinary stones from Southern India

Role of Tamm-Horsfall protein and uromodulin in calcium oxalate crystallization

Überwachung des Behandlungserfolgs der bei Kalziumoxalatsteinmetaphylaxe mit dem Bonn-Risk-Index

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