In vitro conservation of chestnut (Castanea sativa) by slow growth

In vitro slow growth storage of Senecio macrophyllus shoots

“…A benefit for shoot survival is also displayed by ABA or/and proline pretreatment of Brassica campestris and B. napus (Wilen et al 1994), and Begonia x erythrophylla (Burritt 2008). However, pretreatment of (Capuana and Di Lonardo 2013). In the case of S. macrophyllus the addition of ABA to storage medium stimulated survival and higher proliferation of shoots during regrowth in optimal conditions in comparison to shoots stored on a medium without ABA.…”

“…However, many species growing well in this climate zone are chill-sensitive and somewhat higher temperatures of 6-12°C are used for them. Better results of storage were achieved at a temperature of 8°C for Castanea sativa (Capuana and Di Lonardo 2013), but for Beta vulgaris, Solanum sp and Vitis rupesttris a temperature of 9-12°C was used (Grout 1995). During storage of the shoot tips of S. macrophyllus a temperature of 10°C was applied.…”

“…Storage in low levels of illumination (2-20 lmol m -2 s -1 ) was reported for Fragaria (Reed 2002), Lilium (Bonnier and Van Tuyl 1997), Hosta (Gollagunta et al 2005), and Solanum (Sarkar and Naik 1998). However, shoots of Castanea sativa were stored under the conditions of only 30 % reduced illumination (Capuana and Di Lonardo 2013), but shoots of Podophyllum peltatum in a light level of approximately 52 lmol m -2 s -1 (Lata et al 2010). ABA is known as a growth retardant on in vitro growth.…”

Conservación de plantas regeneradas in vitro y análisis de la variación somaclonal de Cinchona officinalis, Linneo

“…La presencia de coloración, ya sea verde o amarillenta (clorótica), por lo general demuestra que existen signos de actividad metabólica evidenciada por la presencia de clorofila (García et al 2002). El contenido del pigmento clorofila ha sido utilizado como un indicador de actividad metabólica en Castanea sativa durante el proceso de conservación (Capuana & Di Lonardo 2013), siendo un indicador asociado a la vitalidad de los tejidos.…”

Application of Biotechnology in the Conservation of the Genus Castanea

“…Within these methods, the in vitro culture techniques of plant tissue are considered very effective for the conservation of germplasm, especially in the case of rare or threatened species [21]. These techniques have been developed as an alternative to the conventional propagation methods that, as we have already mentioned previously, are costly, long, and in many cases, not very effective.…”