Conservación de plantas regeneradas in vitro y análisis de la variación somaclonal de Cinchona officinalis, Linneo

Analysis of the Somaclonal Variation in Two in Vitro Regenerated Agave Species

“…It is known that the development pattern of an explant during morphogenesis in vitro is a key element related to SV since, when a highly differentiated tissue passes through a stage of dedifferentiation with a high rate of cell division, more SV can occur than when regeneration develops directly from axillary buds or embryos (Cardone et al, 2004;Sahijram et al, 2003). This can explain the results obtained in our study since, although efficient results were obtained in sprouting with the indirect organogenesis system in both species (Arzate-Fernandez et al, 2020), genetic analysis with both types of molecular markers found instability in all the clones that passed through a phase of indirect tissue organogenesis (Armijos- González, 2016;Oliveira et al, 1995). Moreover, it can also be explained by the heterogeneity of the callus cells and the possible accumulation of genomic alterations (Kuznetsova et al, 2006) during long-term culture (Bublyk et al, 2012).…”

“…This may be due to an increase in the duration of exposure to stress-causing factors, such as plant growth regulators (PGR). Several studies have reported that mutations accumulate sequentially with culture time; regenerated plants cultured for three months can contain a small number of mutations, and after several subcultures, mutations can occur (Armijos- González, 2016;Kaeppler et al, 2000;Peng et al, 2015). This point can be another possible factor that may have affected our results since, in the case of IO regenerated plantlets, the treatment of callus induction lasted 60 days (two subcultures), the treatments of shoot regeneration 60 days more and plantlets passed 45 days in a rooting medium.…”