In vitro slow growth storage of Senecio macrophyllus shoots

Trejgell, Alina; Kamińska, M.; Tretyn, Andrzej

doi:10.1007/s11738-015-1983-8

Cited by 18 publications

(9 citation statements)

References 27 publications

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“…Our results showed that the conservation period has a significant effect on the percentage of cultures vitality in the laboratory to maintain the ability to multiply and rooting, as this effect varied according to the strength of medium, as it was noticed that the conservation period of 3 and 6 months had good results, if compared to the 9-month conservation period, and with the increase in the conservation period to 9 months, the viability percentage and ability to multiply and rooting decreased. These results are in line with what Trejgell et al (2015) found when storing Senecio macrophyllus shoots in vitro for three and six months.…”

Section: Discussionsupporting

confidence: 92%

Minimal Media Strength for In vitro Conservation of Strawberry (Fragaria ananassa) Cultures

Kadhim

Abdulhussein

2021

Basrah J. Agric. Sci.

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An experiment for strawberry Fragaria ananassa cv. Festival in vitro conservation of cultures for nine months was studied. Three MS medium strength (full strength MS, ¾ MS, ½ MS, and ¼ MS) and the storage period (3, 6, and 9 months) in slowing down or reducing growth. Results showed a significant effect of the medium salt strength, as the full strength treatment was higher by achieved the highest percentage of viability (survival %) and storage characteristics of cultures (N%, P%, carbohydrates%, protein %) and regeneration ability characteristics (shoots and roots number and length). Three-month storage period achieved the highest rate compared to the nine-month storage period, which recorded the lowest average for the studied characteristics. The full strength treatment for three months storage period realized the highest rate compared to treatment ¼ strength for the storage period of nine months, which recorded the lowest rate of the studied traits. The application of minimal strength for in vitro conservation allows strawberry cultures to remain viable with a slow growth rate and a significant increase in the storage life of the tissues to nine months.

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Section: Discussionsupporting

confidence: 92%

Minimal Media Strength for In vitro Conservation of Strawberry (Fragaria ananassa) Cultures

Kadhim

Abdulhussein

2021

Basrah J. Agric. Sci.

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“…Similar results were obtained for the shoots of Pyrus genotypes where incubation at low temperature and light intensity resulted in yellowing of shoots, leaf size became very small but the shoot tips were quite fresh and green (Ahmed et al 2010). In our previous studies on the storage of Senecio macrophyllus light also had a negative effect on shoots condition during storage, and addition of ABA to the medium improved their condition (Trejgell et al 2015). Poplar shoots were in better condition after storage in the dark than under light (Hausman et al 1994).…”

Section: Discussionmentioning

confidence: 83%

Effect of light conditions and ABA on cold storage and post-storage propagation of Taraxacum pieninicum

Kamińska

Skrzypek

Wilmowicz

et al. 2016

Plant Cell Tiss Organ Cult

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Taraxacum pieninicum is an extremely threatened species. It seems that traditional protection methods of this species are insufficient and it is necessary to use biotechnology tools that allow the in vitro storage of plant material. The present study describes in vitro conservation of T. pieninicum by slow-growth storage. Various light conditions and abscisic acid (ABA) treatments and combinations thereof were tested. Viability, proliferation rate and ability of shoots to root were evaluated during regrowth. Moreover, the effect of shoot storage conditions on the ABA level was analysed. The results showed that light during 3-months' storage increased the level of endogenous ABA. Similar results were observed when storage at a low temperature was prolonged to 9 months. Changes in ABA level had a negative effect on shoot condition. However, these changes were related to leaves and did not affect the ability of shoot tips to proliferate after long-term storage. Addition of ABA to storage medium increased several folds the level of ABA in plant tissues, which resulted in a reduction in the visual rating and proliferation rate. Shoots obtained after post-storage regrowth were able to root. All rooted shoots survived adaptation to field conditions and were able to flower in the second year after acclimatization. The analysis of DNA content indicated that all the regenerants had the same ploidy level independent of treatments during storage. Cold storage of T. pieninicum as used in this study enabled the interval between subcultures to be extended to 9 months.

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“…The visual rating of the stored shoots was evaluated directly after cold treatment. Shoots were rated on the scale from 0 to 5, on the basis of plant appearance (modified scale from Reed 1992, for details see Trejgell et al 2015). Shoot tips from converted plants were transferred on fresh MS medium with full-strength nutrients supplemented with 3% sucrose, 1.11 μM BA, 0.14 μM NAA and 0.8% agar in optimal growth conditions and subcultured twice.…”

Section: Post-storage Regrowthmentioning

confidence: 99%

Efficient long-term conservation of Taraxacum pieninicum synthetic seeds in slow growth conditions

Kamińska

Gołębiewski

Tretyn

et al. 2017

Plant Cell Tiss Organ Cult

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The aim of this paper was to develop a protocol for efficient storage of artificial seeds of Taraxacum pieninicum, critically endangered Asteraceae species. Storage under reduced light conditions or in the darkness was tested on a basis of synthetic seeds ability to conversion and post-storage regrowth of shoot tips. The results indicated that synseeds obtained from shoot tips of T. pieninicum can be stored at 4 °C even for 12 months without subculture. The light is a stress factor during storage what was manifested by numerous necrosis and decreased shoots ability to proliferate in optimal growth conditions in 1st subculture. Additionally our results showed that the storage does not produce genetic variation at the resolution provided by the flow cytometry and RAPD analysis.

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In vitro slow growth storage of Senecio macrophyllus shoots

Cited by 18 publications

References 27 publications

Minimal Media Strength for In vitro Conservation of Strawberry (Fragaria ananassa) Cultures

Minimal Media Strength for In vitro Conservation of Strawberry (Fragaria ananassa) Cultures

Effect of light conditions and ABA on cold storage and post-storage propagation of Taraxacum pieninicum

Efficient long-term conservation of Taraxacum pieninicum synthetic seeds in slow growth conditions

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