In Vitro Biocompatibility and Oxidative Stress Profiles of Different Hydraulic Calcium Silicate Cements

Abstract

Introduction MTA Plus (MTAP; Avalon Biomed Inc., Bradenton, FL) is a new calcium silicate cement with unknown cytotoxicity characteristics. The objectives of this study were to examine the effect of MTA Plus on the viability, apoptosis/necrosis profile and oxidative stress levels of rat odontoblast-like cells. Methods MDPC-23 cells were exposed to gray and white MTA Plus (GMTAP, WMTAP), gray and white ProRoot® MTA (GMTA, WMTA; Dentsply Tulsa Dental Specialties, Tulsa, OK) cements or their eluents. The cells were evaluated for: i) cell viability using XTT assay, ii) apoptosis/necrosis using flow cytometry and confocal laser scanning microscopy, and iii) oxidative stress by measuring reactive oxygen species. Results XTT assay showed that all test cements exhibited marked initial cytotoxicity that decreased with time. By the end of the third week, GMTAP and GMTA were comparable to untreated cells (negative control) in terms of cell viability, while WMTAP and WMTP were significantly lower than the untreated cells. Apoptosis/necrosis profiles of cells exposed to WMTAP and GMTAP were not significantly different from untreated cells, while cells exposed to WMTA and GMTA showed significantly less viable cells. All experimental groups exhibited reduction of intracellular ROS formation compared to untreated cells, although cells exposed to WMTA was not significantly different from untreated cells. Conclusions Both the gray and white versions of MTA Plus possess negligible in-vitro cytotoxic risks that are time and dilution dependent. They enrich the spectrum of hydraulic calcium silicate cements currently available to clinicians for endodontic applications.

Human dental pulp cells response to mineral trioxide aggregate (MTA) and MTA Plus: cytotoxicity and gene expression analysis

“…They are also consistent with a study included in the XTT assay that MTA Plus with 1 : 5 and 1 : 10 dilutions had no toxic effects on MDPC‐23 cells (Eid et al . ).…”

Effects of tricalcium silicate cements on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells in vitro

“…To avoid the initial cytotoxic effects of TSCs as demonstrated in a previous study [26], a previously-published aging/elution protocol based on the cements' specific cytotoxic profiles was adopted to render the cement discs non-cytotoxic prior to initiation of the experiment [26, 27]. According to this protocol, cement discs were sterilized by UV light for 4 hours, aseptically placed in their respective sterile Transwell® inserts (3.0 micron porous filter, Corning Inc., MA), and immersed in complete culture medium (CCM) for 2 weeks at 37°C in 100% humidity.…”

Hyperglycemic condition influence on mineral trioxide aggregate biocompatibility and biomineralization

“…Histological assessment of them indicated that although both materials were well tolerated, supported cell attachment, proliferation and matrix formation, more inflammation was associated with WMTA (Vosoughhosseini et al, ). Using MDPC‐23 cells and XTT assay, GMTA had higher cell viability than WMTA (Eid et al, ).…”