Human dental pulp cells response to mineral trioxide aggregate (<scp>MTA</scp>) and <scp>MTA</scp> Plus: cytotoxicity and gene expression analysis

Rodrigues, Érico; Cornélio, A. L. G.; Mestieri, Letícia Boldrin; Fuentes, Andrea S.C.; Salles, Loise Pedrosa; Rossa-Junior, Carlos; Faria, Gisele; Guerreiro-Tanomaru, Juliane Maria; Tanomaru‐Filho, Mário

doi:10.1111/iej.12683

Cited by 50 publications

(52 citation statements)

References 40 publications

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“…All of the tested materials maintained cell viability and proliferation, and no evidence of damage to the cell membrane and DNA was found. Corroborating with our findings, previous studies have shown that ProRoot MTA, MTA-Angelus and PC were not cytotoxic, allowing a cell viability of 70% or higher, as required by the ISO guidelines (4,(30)(31)(32)(33).…”

Section: Discussionsupporting

confidence: 91%

Physico-chemical and Biological Properties of a New Portland Cement-based Root Repair Material

Aminsobhani¹,

MERAJI²,

Khoshdel³

et al. 2017

Eur Endod J

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Objective: To propose bismuth carbonate, a radiopacifying agent, as a new endodontic root repair material that was added to Portland cement (PC) at 2 wt%, 5 wt%, 10 wt% and 15 wt%, and physicochemical and biological properties of each formulation were evaluated in comparison to MTA-Angelus. Methods: Mixed and powder samples were analysed by scanning electron microscopy (SEM) and X-ray power diffraction (XRD), and the semiquantitative constitution of the powder was determined by energy-dispersive spectrometer (EDS). Setting time was evaluated by Vicat needle and radiopacity analysed with digital X-ray. The pH of all tested materials was observed after immersion in water for 3, 24, 48, 72 and 168 h (or 7 days). Solubility and calcium release were measured after immersion in water for 24 h. A multiparametric assay XTT-NR-CVDE was used to evaluate the cytotoxicity of the materials in human periodontal ligament (HPDL) fibroblasts. HPDL fibroblasts were exposed to PC 15% and mineral trioxide aggregate (MTA), and the expression of proinflammatory cytokines (IL1A, IL6, IL8, TNF) and bone formation genes (ALP, COL1 , RUNX2) was evaluated by real-time PCR. Mineralisation of HPDL fibroblasts cocultured with PC, PC 15% and MTA was evaluated with Von Kossa staining. Results: PC-based groups presented more irregular and larger particles than MTA. PC and MTA showed similarities as observed by XRD and EDS. Setting time of PC-based groups was increased with the addition of bismuth carbonate. All tested materials were alkaline, and pH tended to reduce over time. All cements had solubility lower than recommended, with no difference between them (P>0.05) and showed calcium release. PC 15% had similar radiopacity when compared with MTA (P>0.05). Cell viability was higher for the tested materials than the positive control (P<0.001), but there was no difference when they were compared with negative control (P>0.05). Gene expression levels were similar for all tested groups (P>0.05). Analysed cements had positive Von Kossa staining. Conclusion: Overall, the addition of 15% of bismuth carbonate did not result in significant changes to its physicochemical and biological properties when compared with MTA, except for the setting time, and may be considered a potential substitute for MTA. Keywords: Bismuth carbonate, mineral trioxide aggregate, portland cement, root repair material INTRODUCTIONMineral trioxide aggregate (MTA) is a root repair material with Portland cement (PC) and bismuth oxide as major components (1). Several studies have shown that both MTA and PC present similar physicochemical and biological properties (2-5). PC has been proposed as an alternative material to MTA, but its major disadvantage is that it does not achieve the radiopacity level required to be distinguished from the surrounding anatomical structures, thus requiring the addition of a radiopacifying agent prior to its use as an endodontic material (6).Previous studies have shown that bismuth oxide, the radiopacifying agent in ProRoot MTA and MTA-Angelus, decrea...

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Section: Discussionsupporting

confidence: 91%

Physico-chemical and Biological Properties of a New Portland Cement-based Root Repair Material

Aminsobhani¹,

MERAJI²,

Khoshdel³

et al. 2017

Eur Endod J

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“…Production of OCN by osteoblastic cells in contact with MTA has been demonstrated in vitro (Tani‐Ishii et al ). In addition, MTA has been shown to induce the expression of the BMP‐2 and OCN osteogenic markers in dental pulp cells (Rodrigues et al ) and both MTA and Biodentine™ induced BMP‐2 expression in osteogenic cells and were considered adequate materials to stimulate the mineralization by osteoblastic cells (Rodrigues et al ). BSP is also involved in the formation of the mineralized matrix, and the expression of this protein is highly specific for mineralized tissues including bone, cartilage, dentine and cementum (Tani‐Ishii et al ).…”

Section: Discussionmentioning

confidence: 99%

Histopathological, histoenzymological, immunohistochemical and immunofluorescence analysis of tissue response to sealing materials after furcation perforation

et al. 2019

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Aim To evaluate in vivo tissue responses after sealing furcation perforations in dog's teeth with either Biodentine™, mineral trioxide aggregate (MTA) or gutta‐percha, by means of histopathological, histoenzymological, immunohistochemical and immunofluorescence analysis. Methodology After root canal treatment, perforations were created in the central region of the pulp chamber floor using a round diamond bur and filled with one or other of the materials. The animals were euthanized after 120 days, and the teeth (n = 30) were processed for histopathological analysis of new mineralized tissue formation and collagen fibre reinsertion, immunohistochemical analysis of osteopontin (OPN) and alkaline phosphatase (ALP) and immunofluorescence analysis for bone morphogenetic protein (BMP‐2), cementum attachment protein (CAP), bone sialoprotein (BSP), osteocalcin (OCN) and cementum protein1 (CEMP1). Histoenzymology was performed for TRAP activity and osteoclast count. Data were analysed statistically (α = 0.05) using chi‐square and Kruskal–Wallis tests. Results Gutta‐percha did not induce mineralized tissue formation. MTA and BiodentineTM formed mineralized tissue in 88% and 92% of specimens, respectively, with no significant difference (P > 0.05). Gutta‐percha was associated with scattered collagen fibres parallel to the perforations. Groups treated with MTA or BiodentineTM had partial fibre reinsertion perpendicular to the newly formed mineralized tissue. All materials induced OPN and ALP expression, weakest for gutta‐percha and strongest for MTA (P < 0.05). Only MTA induced BMP‐2, BSP, OCN, CAP and CEMP1 expression. Osteoclast counts were similar in all groups (P = 0.97). Conclusions Mineral trioxide aggregate and BiodentineTM were biocompatible, with formation of mineralized tissue and partial reinsertion of collagen fibres. In addition, the participation of several molecules by which calcium silicate‐based materials induce the formation of mineralized tissue were noted, with expression of ALP and OPN mineralization markers, without interference in the number of osteoclasts. Only MTA stimulated the expression of proteins associated with the formation of a cementum‐like mineralized tissue.

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“…Four in vitro studies addressing the bio activity and cytotoxicity of the materials used as cervical barriers were selected. [6][7][8][9] Regarding the publication years and journals, one article from 2014 was found in the Journal of Endodontics, 6 one article from 2015 in the Dental Materials, 9 and two articles from 2017 in the International Endodontic Journal 7 and the Journal of Medical Sciences. 8 The findings are shown in Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…2,3,10,18 Among pulp revascularization stages, this study assessed the use of different materials used as cervical barrier. Of the materials evaluated in this study, articles were found using white and gray MTA, [6][7][8][9][10][11][12][13][14][15][16] Biodentine, 9,14,15,18 EndoSequence, 14,15 TheraCal, 9 and the Calcium-Enriched Mixture (CEM). 4,8,17 The materials used in cervical sealing act as physical barrier, protecting the clot and preventing possible recontamination.…”

Section: Discussionmentioning

confidence: 99%

Características dos materiais utilizados como barreira cervical na revascularização pulpar: uma revisão integrativa

Valença

Prado

2018

Rev. Bras. Odontol.

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Objective: to perform an integrative review on the characteristics of materials used as cervical barrier in pulp revascularization, addressing the bioactivity and biocompatibility, as well as the drawbacks inherent to their use. Material and Methods: searches were made, in English and Portuguese, on the platforms PubMed and Google Scholar. The following keywords were used: (pulp revascularization OR pulp regeneration) and (Biodentine OR Endosequence OR MTA Angelus OR Calcium Enriched Mixture OR ProRoot MTA) and (drawing OR discoloration) and (Biodentine OR Endosequence OR MTA Angelus OR Calcium Enriched Mixture OR ProRoot MTA). Inclusion criteria were: articles published from 2008 to 2018, in vitro studies, clinical studies, literature review and case reports. Exclusion criteria were: articles that did not address the topic of revascularization. Results: the electronic search found 337 articles. After title analysis, 81 articles were selected for reading the abstracts, and 66 articles were excluded according to the inclusion criteria. After complete reading of the 15 articles, 2 were excluded for not addressing the topic of this study. The journals found were: to 2017. Conclusion: bioceramic materials used as cervical barrier showed bioactivity and reduced toxicity.As an inconvenience to its use, coronal discoloration is reported in vitro due to MTA, Biodentine and Endosequence, and in vivo only due to MTA. In addition, mineralized tissue may form inside the root canal, possibly due to the bioactivity of these materials.

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Human dental pulp cells response to mineral trioxide aggregate (MTA) and MTA Plus: cytotoxicity and gene expression analysis

Abstract: MTA and MTA Plus were noncytotoxic, increased mineralization processes in vitro and induced the expression of osteogenic markers.

Cited by 50 publications

References 40 publications

Physico-chemical and Biological Properties of a New Portland Cement-based Root Repair Material

Physico-chemical and Biological Properties of a New Portland Cement-based Root Repair Material

Histopathological, histoenzymological, immunohistochemical and immunofluorescence analysis of tissue response to sealing materials after furcation perforation

Características dos materiais utilizados como barreira cervical na revascularização pulpar: uma revisão integrativa

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