In Vitro Attachment of Human Gingival Fibroblasts to Root Surfaces

Aleo, Joseph J.; Renzis, Frank A. De; Farber, Paul A.

doi:10.1902/jop.1975.46.11.639

Cited by 255 publications

(122 citation statements)

References 11 publications

Supporting

Mentioning

107

Contrasting

Unclassified

Order By: Relevance

“…Where the root surface had been treated with a Gracey Scaler, however, poor extensions of filopodia were observed with elongation of the cytoplasmic projections. It has been reported that an acceptable connective tissue attachment is created by performing a thorough scaling and root planing with a hand instrument (25). Such a difference in attachment might be due to the use of more efficacious rotary instruments in the current study.…”

Section: Discussionmentioning

confidence: 74%

Comparison of the effects of various periodontal rotary instruments on surface characteristics of root surface

2004

View full text Add to dashboard Cite

The efficacy of scaling and root planing using various periodontal rotary instruments was examined. Eighty extracted human teeth with a history of periodontal disease were divided into four groups of 20 and subjected to one of the following procedures: Use of 1) a Root Burnisher, 2) a Perio Planing Bur (both rotating instruments for contra angle handpieces), 3) a Tooth Planing Bur (rotating instrument for use with an air turbine), or 4) a Gracey Scaler. In each case, the time required for cleaning was measured. Twenty healthy extracted human teeth were used as untreated controls. After treatment, the surface roughness of 10 specimens out of each group were measured using a profilometer and observed by scanning electron microscopy (SEM). Half of the samples were then incubated in dishes with a suspension of fibroblasts. After counting the number of attached cells, the attachment of fibroblasts was observed by SEM. The root surfaces treated with the rotary instruments appeared smooth and there were no significant differences between groups. From the SEM observations, smooth root surfaces with different surface textures were evident and a tight attachment of fibroblasts was observed. The results of this study suggest that use of rotary instruments is superior for periodontal scaling and root planing. (J. Oral Sci. 46, [1][2][3][4][5][6][7][8] 2004)

show abstract

Section: Discussionmentioning

confidence: 74%

Comparison of the effects of various periodontal rotary instruments on surface characteristics of root surface

2004

View full text Add to dashboard Cite

show abstract

“…Scaling and root planing seem to effectively remove the endotoxin-contaminated root surface (18). However, animal experimentation (2) and human block sections (2,13,19) indicate that this does not lead to regeneration of a new connective tissue attachment.…”

Section: Discussionmentioning

confidence: 99%

The effects of attachment factors on initial attachment of human periodontal ligament fibroblasts on different root surfaces. A light and scanning electron microscopic study.

Tüter

Yalım

Gürhan

et al. 2000

Journal of Oral Science

View full text Add to dashboard Cite

“…Root debridement by scaling and root planing may not completely remove contaminated cementum particularly in more apical areas and the smear layer formed during root planing inhibits cell migration and attachment leading to impaired marginal periodontal healing. 1 There is considerable interest in the use of chemical agents to assist in root preparation. Agents have been proposed to accomplish the following:…”

Section: Introductionmentioning

confidence: 99%

Effects of Citric Acid and EDTA on Periodontally Involved Root Surfaces: A SEM Study

Prasad

Radharani²,

Varma³

et al. 2012

The Journal of Contemporary Dental Practice

View full text Add to dashboard Cite

Aim: Root debridement by scaling and root planing may not completely remove contaminated cementum particularly in more apical areas. The smear layer formed during root planing inhibits cell migration and attachment leading to impaired marginal periodontal healing. The present study was done to compare the morphological effects of root surface demineralization using citric acid and EDTA as root-conditioning agents. Materials and methods:Thirty fragments of human dental roots previously exposed to periodontal disease were scaled and randomly divided into the following groups of treatment: Group I: Hand instrumentation and conditioning with normal saline for 5 minutes as control; group II: Hand instrumentation and conditioning with citric acid (pH 1) for 5 minutes and group III: Hand instrumentation and conditioning with EDTA (pH-7.4) for 5 minutes. Scanning electron microscopy was used to check for the presence of residual smear layer and for measuring the number and area of exposed dentin tubules. Results:The mean efficacy of smear layer removal was compared between groups I and II, groups I and III and groups II and III, it was found to be statistically significant at 5%. When the mean diameter of the dentinal tubules and mean total surface area occupied by the dentinal tubule orifices was compared between groups II and III it was found to be statistically significant at 1% level of significance. Conclusion:Citric acid causes greater degree of morphological alterations than EDTA and is considered to be a better rootconditioning agent. However, the use of EDTA cannot be ruled out.Clinical significance: This study supports the hypothesis that demineralizing agents can be used as an adjunct to the periodontal treatment aiming at restoring the biocompatibility of roots and helps in choosing an appropriate agent for root conditioning.

show abstract

In Vitro Attachment of Human Gingival Fibroblasts to Root Surfaces

Cited by 255 publications

References 11 publications

Comparison of the effects of various periodontal rotary instruments on surface characteristics of root surface

Comparison of the effects of various periodontal rotary instruments on surface characteristics of root surface

The effects of attachment factors on initial attachment of human periodontal ligament fibroblasts on different root surfaces. A light and scanning electron microscopic study.

Effects of Citric Acid and EDTA on Periodontally Involved Root Surfaces: A SEM Study

Contact Info

Product

Resources

About