In vitro assessment of soybean lecithin and egg yolk based diluents for cryopreservation of goat semen

Salmani, Hossein; Towhidi, Armin; Zhandi, Mahdi; Bahreini, Majid; Sharafi, Mohsen

doi:10.1016/j.cryobiol.2014.02.008

Cited by 84 publications

(73 citation statements)

References 36 publications

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“…Furthermore, neither glycerol nor egg yolk alone could reduce the intact acrosome percentage; however, combination of these two protectants significantly reduces the percentage of intact acrosome spermatozoa [82]. Salmani et al [83] have suggested the use of soyabeanlecithin which is a suitable plant based cryoprotectant for caprine sperms. The beneficial effects of soyabean-lecithin as a substitute for egg yolk during cryopreservation of goat sperm are established in the works of Jiménez-Rabadán et al, Roof et al, Salmani et al, and Vidal et al [84][85][86][87].…”

Section: Cattlementioning

confidence: 99%

“…Samples were frozen in LN 2 vapor, 4 cm above the L, for 7 min; subsequently the straws were plunged into the LN 2 for storage. [83] Blanca-Celtiberica buck Diluted semen was cooled to 5 ∘ C for 2 h, diluted further, and held at 5 ∘ C for 2 h; another sample was cooled to 5 ∘ C for 4 h; both samples were frozen over N 2 vapour for 10 min, 4 cm above N 2 level, plunged, and stored in LN 2 .…”

Section: Goatmentioning

confidence: 99%

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Role of Membrane Lipid Fatty Acids in Sperm Cryopreservation

Mandal

Badyakar

Chakrabarty

2014

Advances in Andrology

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Lipid is an important constituent of cell membrane. Membrane lipid composition of spermatozoa has been correlated to different function. Many researchers have related membrane lipid with survival success after cryopreservation or cold shock. Sperm maturation and acrosome reactions are natural phenomenon, but cryopreservation or cold shock is not. Therefore, sperm cells are not programmed for such change and undergo stress. So the change in membrane lipid composition due to cold shock or cryopreservation may be looked upon as response of spermatozoa to a certain stressed condition. A significant body of research worked on the relationship between membrane lipid and fatty acid composition and ability of cell to tolerate adverse change in temperature. However, as the approach of different research groups was different, it is very difficult to compare the changes. Studies have been done with different species, ejaculated/seminal or epididymal sperm. Lipid analyses have been done with whole cell membrane isolated by different methods. Fatty acids estimated were from whole cell, plasma membrane, head membrane, or phospholipids. The cryopreservation condition, media composition, and diluents/cryoprotectants were also different. At this onset a comprehensive review is needed to cover changes of sperm membrane lipid composition of different species under different cryopreservation conditions.

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Section: Cattlementioning

confidence: 99%

Section: Goatmentioning

confidence: 99%

Role of Membrane Lipid Fatty Acids in Sperm Cryopreservation

Mandal

Badyakar

Chakrabarty

2014

Advances in Andrology

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“…The basic extender was prepared by mixing 300 mM Tris, 28 mM glucose, 95 mM citric acid, 5% glycerol (v/v), and 500 µg/ ml gentamicin in distilled water respectively. Three different treatment extenders were prepared by adding 1%, 1.5% and 2% Soybean Lecithin (w/v) with 100 ml of basic extender and control extender was prepared by adding 15% egg yolk (Salmani et al, 2014). After preparation extender was kept in water bath at 37° C for semen dilution.…”

Section: Methodsmentioning

confidence: 99%

“…In similar to our findings, 1% SL was reported optimal for cryopreservation of ram (Sharafi et al, 2009 andForouzanfar et al, 2010) and human semen. Salmani et al (2014) observed increase of soybean lecithin level above 1.5% in the extender leads to reduce sperm quality after freezing-thawing process. Forouzanfar et al (2010) reported that high concentration of soybean lecithin was toxic for sperm motility and viability.…”

Section: Functional Membrane Integritymentioning

confidence: 99%

“…The first mechanism for protective effects of soybean lecithin is described by Bergeron and Manjunath (2006) suggested an interaction between seminal plasma proteins and LDL in extenders. Furthermore, the influential antioxidant compounds in soy lecithin as glutathione may protect the sperm viability via scavenging the LPO and prevention of MDA formation during cooling or freezing process (Salmani et al, 2014).…”

Section: Functional Membrane Integritymentioning

confidence: 99%

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Preservation of Black Bengal buck semen in soybean lecithin based chemically defined extender

Konyak

Mandal

Mondal

et al. 2018

IJAR

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This experiment was conducted with the aim to study the effect of replacing egg yolk with soybean lecithin (SL) for cryopreservation of Black Bengal buck semen. Sexually matured Black Bengal buck (n = 5) were used and the ejaculates were obtained using an artificial vagina method. The semen samples were pooled and diluted in Tris extender with 5% Glycerol containing either 15% egg yolk (control group) or SL at different concentrations (1% SL, 1.5% SL and 2% SL). The semen samples were filled in straws and cooled gradually to 5 °C. Semen straws were equilibrated for 3 hours at 5°C and were frozen in static liquid nitrogen vapor and stored in liquid nitrogen. Semen samples were evaluated after initial dilution, after completion of equilibration and after freeze thawing for in vitro sperm characters such as sperm motility, functional membrane integrity and malondioldehyde (MDA) concentration. Semen samples preserved in extender containing 1% SL was able to maintain in vitro sperm characters similar to the extender containing egg yolk. However, significant (P<0.05) reduction in all semen parameters was observed as the concentration of soybean lecithin increased above 1% level. It is concluded that an extender containing soybean lecithin @ 1% with 5% Glycerol can be used for replacing egg yolk for cryopreservation of Black Bengal buck semen.

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Effects of egg yolk and soybean lecithin on sperm quality determined by computer‐assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm

Nguyen

Ponchunchoovong

Kupittayanant

et al. 2019

Veterinary Medicine & Sci

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The reduction of spermatozoa survival time is a major problem of canine chilled sperm for artificial insemination. The aim of the study was to improve the quality of canine chilled sperm during storage time. We therefore, evaluated the effects of eight treatments with different levels of soybean lecithin concentration (1, 3 and 5%) and egg yolk (20%) in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extender on chilled canine sperm quality during 10 days of storage. The sperm motility was analysed by computer‐assisted sperm analysis (CASA), whereas plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential parameters were determined using a fluorescent staining combination of propidium iodide (PI), Hoechst 33342 (H342), fluorescein isothiocyanate‐conjugated Pisum sativum agglutinin (FITC‐PSA) and 5,5′,6,6′‐tetrachloro‐1,1′,3,3′‐tetraethylbenzimidazolyl‐carbocyanine iodide (JC‐1) by confocal laser scanning microscope. The results showed that egg yolk was found to be better than soybean lecithin in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extender for maintaining the quality of chilled canine sperm within 10 days of storage (P < 0.05). Although egg yolk in Tris‐citric‐fructose extender could maintain the motility better than other extenders, egg yolk in Tris‐citric‐fructose‐mineral salts extender was the highest in intact plasma membrane, intact acrosome membrane and high mitochondrial membrane potential (P < 0.05). In contrast, the sperm quality of soybean lecithin in Tris‐citric‐fructose‐mineral salts extender was lower than that of soybean lecithin in Tris‐citric‐fructose extender, and soybean lecithin 1% was greater than soybean lecithin 3% and 5% in plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential (P < 0.05). In conclusion, soybean lecithin cannot replace egg yolk in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extenders, and egg yolk in Tris‐citric‐fructose‐mineral salts extender is superior to other extenders in chilling canine sperm.

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In vitro assessment of soybean lecithin and egg yolk based diluents for cryopreservation of goat semen

Cited by 84 publications

References 36 publications

Role of Membrane Lipid Fatty Acids in Sperm Cryopreservation

Role of Membrane Lipid Fatty Acids in Sperm Cryopreservation

Preservation of Black Bengal buck semen in soybean lecithin based chemically defined extender

Effects of egg yolk and soybean lecithin on sperm quality determined by computer‐assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm

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