Preservation of Black Bengal buck semen in soybean lecithin based chemically defined extender

Abstract

This experiment was conducted with the aim to study the effect of replacing egg yolk with soybean lecithin (SL) for cryopreservation of Black Bengal buck semen. Sexually matured Black Bengal buck (n = 5) were used and the ejaculates were obtained using an artificial vagina method. The semen samples were pooled and diluted in Tris extender with 5% Glycerol containing either 15% egg yolk (control group) or SL at different concentrations (1% SL, 1.5% SL and 2% SL). The semen samples were filled in straws and cooled gradually to 5 °C. Semen straws were equilibrated for 3 hours at 5°C and were frozen in static liquid nitrogen vapor and stored in liquid nitrogen. Semen samples were evaluated after initial dilution, after completion of equilibration and after freeze thawing for in vitro sperm characters such as sperm motility, functional membrane integrity and malondioldehyde (MDA) concentration. Semen samples preserved in extender containing 1% SL was able to maintain in vitro sperm characters similar to the extender containing egg yolk. However, significant (P<0.05) reduction in all semen parameters was observed as the concentration of soybean lecithin increased above 1% level. It is concluded that an extender containing soybean lecithin @ 1% with 5% Glycerol can be used for replacing egg yolk for cryopreservation of Black Bengal buck semen.

Electrophoretic profile of seminal proteins and their correlation with in vitro sperm characters in Black Bengal buck semen

“…Semen ejaculates were collected twice a week using artificial vagina. Before semen collection, bucks were sexually prepared by allowing 1-2 false mounts [4,5]. On every collection day, two ejaculates were collected from each buck with a brief interval.…”

“…Neat semen samples were evaluated for volume, sperm cell concentration (hemocytometer method), mass activity, abnormal count (Rose Bengal staining method), individual motility, and functional membrane integrity (hypo-osmotic swelling test [HOST]) immediately after collection [4,5]. After the initial evaluation, semen ejaculates were diluted 1:5 with Tris-fructose-citric acid-egg yolk-glycerol buffer and equilibrated at refrigeration temperature for 3 h. Filling and sealing of straws were done manually in French Mini Straws during the equilibration period.…”

“…Artificial insemination (AI) technology has made possible the safe use of semen from selected sires in a large breeding female population. AI in goat is gaining popularity in several states of India such as Tamil Nadu, Kerala, West Bengal, Assam, and others for the past few years [4-6]. AI costs less when compared to keeping breeding buck(s) in small flocks of 3-5 goats, and the farmer has access to a wide variety of quality bucks of high genetic merit at relatively low cost.…”

In vitro Development of Preimplantation Caprine Embryo using Cryopreserved Black Bengal Buck Semen

“…In this experiment frozen buck semen (Konyak et al, 2018) was used for IVF and all three different culture media (RVCL, mSOF, KSOM) were used for embryo development. Table.2).…”

Heparin Binding Proteins of Black Bengal Buck Semen and Their Correlation with Sperm Characters and Freezability

“…AI costs less when compared to keeping breeding buck(s) in small flocks of less than 10 goats and the farmers will have access to quality elite buck semen. AI in goat is gaining popularity for the last few years in India (Konyak et al, 2018 andKarunakaran et al, 2019). While adapting AI technology, the buck selected as semen donor should have acceptable fertility, as it influences the reproductive potential of large female population.…”