Electrophoretic profile of seminal proteins and their correlation with in vitro sperm characters in Black Bengal buck semen

Karunakaran, M.; Gajare, Vivek C.; Mandal, Ajoy; Mondal, Mohan; Das, Subrata K.; Ghosh, Manoj Kumer; Rai, Sweta; Behera, Rajalaxmi

doi:10.14202/vetworld.2019.621-628

Cited by 13 publications

(12 citation statements)

References 39 publications

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“…Similar to our findings of individual variation in all parameters except for sperm viability and abnormalities, individual variation in semen volume, sperm motility and viability, mass movements, sperm concentration, membranes integrity, acrosome integrity [9], and sperm abnormalities [39] has been found in goats as well as in boar sperm motility, MI, and mitochondrial activity [40].…”

Section: Individual Variation In the Quality Of Fresh Semensupporting

confidence: 90%

Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus)

et al. 2020

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Aim: This study aimed to analyze the individual factors influencing the sperm quality of Bali bulls at Baturiti Artificial Insemination (AI) center. Materials and Methods: Semen that was ejaculated from nine Bali bulls was collected using artificial vaginas (n=5/bull). Semen ejaculates were evaluated immediately after collection to measure the quality of the fresh semen, including semen volume, sperm concentration, progressive motility, membrane integrity (MI), and abnormal morphology. Frozen semen was evaluated for progressive sperm motility, concentration, viability, MI, abnormal morphology, and deoxyribonucleic acid (DNA) fragmentation. Other secondary data, focusing on semen quantity (semen volume and sperm concentration), were also collected from frozen the semen production data of the Baturiti AI center from 2017 to 2019. Data were analyzed statistically using a completely randomized design, and one-way analysis of variance was applied to find differences among individual bulls. Results: Significant differences (p<0.05) were found among the bulls in semen volume, sperm motility, concentration, and MI of the fresh semen. Significant differences (p<0.05) were also found among the bulls in sperm motility, viability, MI, abnormal morphology, and DNA fragmentation of the frozen semen. Conclusion: Individual variation in all the tested sperm parameters of the fresh semen of Bali bulls, except sperm viability and abnormalities, was noted. Similarly, individual variation in all the tested sperm parameters in frozen semen, except sperm concentration, was noted. Therefore, individual factors can be used for selecting a superior bull in Bali cattle.

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Section: Individual Variation In the Quality Of Fresh Semensupporting

confidence: 90%

Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus)

et al. 2020

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“…The sperm motility percentage was categorized normal based on the SNI 4869-1:2017 requirement for post-thawing frozen semen with minimum mobility of 40% [20]. Karunakaran et al [8] showed that generally after the process of freezing and thawing, sperm motility decreases by around 34%-46%. Cryopreservation and the freeze-thawing phase causes detrimental effects, such as stress induced by chemical, physical, and osmotic pressure, or changes in the temperature of the sperm [21].…”

Section: Discussion the Post-freeze Thawing Quality Of Holstein Bull Sperm And The Correlation With Fertility Levelmentioning

confidence: 99%

“…A specific protein in the semen can change its function and affect the fertility of sperm [7]. It is reported that several proteins in the sperm and seminal plasma could be used as indicators of sperm characters, quality, and fertility levels [7,8]. Detected proteins in the sperm are related to the bull's fertility, such as a heparin-binding protein with a molecular weight (MW) of 31 kDa [9] and phospholipase A2 with an MW of 16 kDa [10].…”

Section: Introductionmentioning

confidence: 99%

Sperm protein markers for Holstein bull fertility at National Artificial Insemination Centers in Indonesia

et al. 2020

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Background and Aim: Holstein cows and heifers are widely bred in Indonesia by artificial insemination (AI) to increase population and milk production. Sperm fertility is modulated by genetic factors, but the analysis of sperm quality is still based on macro- and microscopic characteristics. This study aimed to analyze both sperm quality and proteins of Holstein bulls at different fertility levels. Materials and Methods: The frozen semen samples were collected from the Indonesia National AI Center. They were classified based on the reproductive efficiency data and were grouped into high fertile (HF) and low fertile (LF). Sperm qualities were evaluated by microscopic evaluation. The Holstein sperm proteins were extracted using phenylmethanesulfonyl fluoride as a protease inhibitor and the benzidine detergent extraction method. Discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was conducted to analyze the molecular weights (MWs) of the sperm proteins. The data obtained were analyzed by a t-test using the one-factor bull fertility level, and Spearman's correlation analysis was used to identify the correlation between the sperm microscopic evaluation parameters and protein bands. Results: The sperm motility post-freeze thawing was not significantly different between the HF and LF (p>0.05). The HF level had a higher percentage of viability, intact plasma membrane integrity, and intact acrosomes than the LF (p<0.05). Five protein bands were found in the SDS-PAGE of sperm proteins of Holstein bulls with different concentrations. Sperm proteins with MWs of 17.51 kDa, 14.87 kDa, 33.71 kDa, and 41.97 kDa were abundant in the Holstein bulls with an HF level, while 55 kDa proteins were abundant in the LF level of Holstein bulls. The sperm of Holstein bulls in the HF level contained proteins of about 33.71 kDa that were not detected in the LF. Conclusion: The sperm protein with a molecular weight of 33.71 kDa was predicted to be a specific protein biomarker that influences bull fertility. Sperm fertilization abilities were also determined by the sperm proteins, the morphology of sperm acrosomes, and the quality of plasma membranes. This method can be used to select bulls with high fertility to increase the population of Holstein bulls.

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“…Other studies conducted in bull, ram and buck revealed that quantitative variations in seminal plasma and sperm proteins also appeared between males [90][91][92][93][94][95][96], which could explain in part the individual variability observed in sperm freezability [97,98]. Males are frequently categorized as good or bad freezers depending on their post-thaw sperm quality; however, molecular biomarkers could predict more efficiently sperm cryotolerance before freezing-thawing in different individuals [10].…”

Section: Molecular Mechanisms Involved In Those Factors That May Affementioning

confidence: 99%

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Peris-Frau¹,

Soler²,

Iniesta‐Cuerda³

et al. 2020

IJMS

139

118

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Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm cryopreservation in different ruminant species. However, a significant amount of sperm still suffers considerable cryodamage, which may affect sperm quality and fertility. Recently, the use of different “omics” technologies in sperm cryobiology, especially proteomics studies, has led to a better understanding of the molecular modifications induced by sperm cryopreservation, facilitating the identification of different freezability biomarkers and certain proteins that can be added before cryopreservation to enhance sperm cryosurvival. This review provides an updated overview of the molecular mechanisms involved in sperm cryodamage, which are in part responsible for the structural, functional and fertility changes observed in frozen–thawed ruminant sperm. Moreover, the molecular basis of those factors that can affect the sperm freezing resilience of different ruminant species is also discussed as well as the molecular aspects of those novel strategies that have been developed to reduce sperm cryodamage, including new cryoprotectants, antioxidants, proteins, nanoparticles and vitrification.

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Electrophoretic profile of seminal proteins and their correlation with in vitro sperm characters in Black Bengal buck semen

Cited by 13 publications

References 39 publications

Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus)

Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus)

Sperm protein markers for Holstein bull fertility at National Artificial Insemination Centers in Indonesia

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

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