In vitro antioxidant and anticancer activity of Leea indica leaf extracts on human prostate cancer cell lines

Abstract

BackgroundTo determine the phytochemical constituents, antioxidant, and anticancer activities of Leea indica leaf extracts on DU-145 and PC-3 human prostate cancer cell lines.MethodsLeaf sample was subjected to Soxhlet extraction method with increasing polarity of solvents, namely, chloroform, ethyl acetate, methanol, ethanol, and aqueous. Phytochemical screening was done using different biochemical tests. Quantitative analysis for phenol was determined by Folin–Ciocalteu reagent method. The antioxidant activity was tested using 2,2-diphenyl-1-picrylhydrazyl, ferric ion reducing power assay, and phosphomolybdenum assay. In vitro anticancer activity on DU-145 and PC-3 human prostate cancer cell lines was evaluated by (3-(4, 5-dimethyl thiazole-2yl)-2, 5-diphenyl tetrazolium bromide) MTT assay.ResultsPhytochemical screening confirmed the presence of phyto-constituents like alkaloids, flavonoids, glycosides, phenols, lignins, saponins, sterols, tannins, anthraquinone, and reducing sugar. Methanol and ethanol extracts exhibited higher phenolic content as compare to aqueous extract. Antioxidant capacities were shown highest in methanol and ethanol extracts based on the test performed. The methanol and ethanol leaf extracts were found to be selectively cytotoxic in vitro to (DU-145 and PC-3) prostate cancer cell lines with IC50 values 529.44 ± 42.07 μg/mL and 677.11 ± 37.01 μg/mL for DU-145 and 547.55 ± 33.52 μg/mL and 631.99 ± 50.24 μg/mL for PC-3 respectively, while it had no cytotoxic effect on normal mice embryo fibroblast cells.ConclusionThe results indicate that Leea indica was a promising antioxidant and anticancer agent for DU-145 and PC-3 human prostate cancer cell lines. However, further studies are needed to conclude its therapeutic use.

Bioactive compound fromAspergillus terreusDMTMGK004 synergistically contributes towards potential anti-pathogenicity

“…3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay is frequently used as an in vitro model system to measure cytotoxic effects of bioactive compounds (Ghagane et al . ). AGS gastric cancer cell line and monkey VERO cell line were used to evaluate the selective toxicity of the compound.…”

Evaluation of in vitro Antioxidant and Anticancer Activity of Simarouba glauca Leaf Extracts on T-24 Bladder Cancer Cell Line

“…18 Many species of the Simaroubaceae family display prominent anticancer activity. 19 The plant collected from (ICMR-NITM) Campus, was identified according to their taxonomical characters as Simarouba glauca and analysed for the presences of phytoconstituents with five solvent extracts.…”

Transforming Growth Factor Beta of Testis Germinal Cell in Guinea Pig (Cavia Porcellus) After Exposure to Methanol Extract of the Seeds of Bitter Melon (Momordica Charantia) and Depot Medroxyprogesterone Acetate

“…and several other plants [118][119][120][121]. Beta-sitosterol D-glucoside has many pharmacological activities [118][119][120][121][122][123][124] such as androgenic, antiadenomic, anticancer [129][130][131][132][133][134], antiedemic [135], and antiinflammatory [136][137][138][139][140][141][142]. Sitosterol has a structure such as cholesterol that later in the body can become the precursor of testosterone and The maximum score of the addition is eight [40,94] has been supposed to have an antifertility, and sex steroids have been implicated in the development and maintenance of Benign prostatic hyperplasia (BPH) and use of androgen reducing compounds, such as 5α-reductase inhibitors which block the conversion of testosterone into dihydrotestosterone [14,32,52].…”