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Background: Pre-eclampsia (PE) contributes to the second cause of maternal death in Indonesia. Andaliman is a typical spice of the Batak ethnic in Northern Sumatera Province, Indonesia. This study aimed to explore the potential of novel herbal medicine compound of nanoherbal andaliman and extra virgin olive oil (EVOO) as PE treatment. Methods: Nanoherbal andaliman was generated using High-energy Milling (HEM). The treatments were divided into the following five groups: K- (control): pregnant rats; K+: PE model rats; P1: PE model rats + 0.45 g of EVOO/200 g BW on the 13th–19th day of pregnancy; P2: PE model rats + nanoherbal andaliman 100 mg/200 g BW on the 13th– 19th day of pregnancy; and P3: PE model rats + combination of 0.45 EVOO/200 g BW and nanoherbal andaliman 100 mg/200 g BW on the 13th–19th day of pregnancy. Rats were dissected on the 20th day of pregnancy. The observed parameters were blood pressure, proteinuria, malondialdehyde (MDA), Heat Shock Protein-70 HSP-70 and histology of placenta. Results: A significant difference was noticed (p<0.05) in blood pressure, proteinuria, foetal weight, haematocrit, erythrocytes and trophoblastic cells after the administration of combined nanoherbal andaliman and EVOO. No significant differences in placental weight, foetal number, leukocytes, MDA and HSP-70 were found (p>0.05). Conclusion: The combination of nanoherbal andaliman and EVOO decreased systolic blood pressure and induced the expression of MDA and HSP-70, as well as placental histology of pre-eclamptic rats.

AIM: Free radicals produce from metabolism or enviromental which interact continously with biological system. Picria fel-terrae Lour. herbs have been used as antioxidant and treat various diseases. The aim of this study was to evaluate cytoprotective activity of ethylacetate fraction (EAF) of Picria fel-terrae Lour. herbs. METHODS: Cytoprotective activity were determined by MTT asay and flow cytometry assay on Vero cells which induced with H2O2 0.8 mM. RESULTS: EAF at 100 µg/mL were showed highest viability (88.83 ± 2.90%) and ROS expression (66.75%) on Vero cells. CONCLUSION: EAF of Picria fel-terrae Lour. herbs have cytoprotective activity.

Aim: The aim of this study is to compare whether there are differences in the value content of secondary metabolites (saponins, tannins, and flavonoids) between the group treated with Binahong leaves (administration of colchicines) and the group treated with Binahong leaves without the application of colchicines. Materials and methods:The design of this study is an experimental research laboratory study. This research was done in four different places in which colchicines were applied to improve the quality of Binahong, conducted in the USU Agricultural Faculty's Greenhouse, whereas no application of colchicines was done in the Desa Simpang Pergendangan Kelurahan Tiga Binanga Kabupaten Karo harvesting Binahong plant. The pharmacy faculty of USU was chosen as a place to obtain the extract and the Laboratory of the Research Institute for Spices and Medicinal Plants (Balitro) was where the phytochemical analysis was conducted. Results:The results showed that there were differences in the descriptive values of secondary metabolites in the Binahong leaf extract, such as saponins, tannins, and flavonoids, wherein the group with colchicines was higher than groups without colchicines, but there was no statistical differences between the groups treated with colchicines and those not treated with colchicines (value p > 0.05). ORIGINAL RESEARCH 10.5005/jp-journals-10015-1453Conclusion: Administration of colchicines can improve the content of secondary metabolites in Binahong leaf extract and the plant morphology without affecting the content of the plant extract.Clinical significance: Secondary metabolites in herbal plants, such as saponins, flavonoids, and tannins are able to speed up the healing process. One of the plants that have all three of these active compounds is Binahong (Anredera cordifolia). Binahong leaf extract can stimulate the process of fibroblasts and collagen formation that will accelerate the process of wound healing. Colchicine as a reagent for the mutation is able to affect the number of leaves and morphological characteristics of the Binahong plant.

Abstract-Platelet-Rich Plasma already used by oral surgeon and periodontics due to enhance the bone remodels. Orthodontic tooth movement affects bone remodeling process, bone resorption on the pressure side and new bone formation on the tension side. This is the objective of this research, to observe the effect of PRP to orthodontic tooth movement. This research was conducted in pure clinical experimental to Guinea pig. Nineteen Guinea pigs separated into two groups, control and PRP groups. Blood homolgue from donors was processed became PRP and the highest platelet counted number injected to samples without activator. 4 times measurements were studied: 6, 9, 12 and 24 days of orthodontic tooth movement by rubber separator between central incsicors. Distance between central insicors was measured with digital caliper (0.01 accuracy). Platelet mean measured were 430.0057.32 (10^3/mL). Distance measurements were not significantly different between PRP and control groups at each time point of measurement (p > 0.05) with t-test unpaired analysis, and neither does analyzed with mixed-repeated measured ANOVA (p=0.935) but analysis between 4 times point of measurement and each groups with Greenhouse-Geisser correction showed that mean distance measurements differed significantly between at least three time points measurements (F(2,132;36,243)=3,464, p<0.039). In conclusion to this research, we couldn't see any significantly different between both groups with 4 times points of measurement but there were statistically significant effects of time simultaneously for each groups.