2017
DOI: 10.5530/pj.2017.6.142
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Evaluation of in vitro Antioxidant and Anticancer Activity of Simarouba glauca Leaf Extracts on T-24 Bladder Cancer Cell Line

Abstract: Objective: Screening of preliminary phytochemicals, evaluation of in vitro antioxidant and in vitro anticancer activities of Simarouba glauca leaf extracts on T-24 Bladder cancer cell line. Materials and Methods: Herbal extraction was carried out by Soxhlet method using chloroform, ethylacetate, methanol, ethanol, aqueous and hydroalcohol. Phytochemical investigation was done using biochemical tests. Total phenolic content was estimated by Folin-Ciocalteu reagent (FCR) method. Antioxidant potential of leaf ext… Show more

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Cited by 24 publications
(14 citation statements)
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References 23 publications
(25 reference statements)
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“…reported the phenolic contents of about 200 GA/g and flavonoids as 14.98 mg Q/g in the crude ethanol extract ( Umesh, 2015 ). Another report confirmed the presence of phenolic compounds in ethanol extract as 84.55 ± 0.59 mg GA/g of extract ( Puranik et al., 2017 ). Another group reported a very low level of phenolic and flavonoid compounds in the crude ethanol extract of S. glauca leaves as 0.12 ± 0.01 mg GA/g and 0.14 ± 0.02 mg Q/g of extract respectively ( Osagie-Eweka et al., 2016 ).…”
Section: Resultsmentioning
confidence: 71%
“…reported the phenolic contents of about 200 GA/g and flavonoids as 14.98 mg Q/g in the crude ethanol extract ( Umesh, 2015 ). Another report confirmed the presence of phenolic compounds in ethanol extract as 84.55 ± 0.59 mg GA/g of extract ( Puranik et al., 2017 ). Another group reported a very low level of phenolic and flavonoid compounds in the crude ethanol extract of S. glauca leaves as 0.12 ± 0.01 mg GA/g and 0.14 ± 0.02 mg Q/g of extract respectively ( Osagie-Eweka et al., 2016 ).…”
Section: Resultsmentioning
confidence: 71%
“…Another report from Shimoga Cancer Treatment suggested the use of decoction prepared from Simarouba glauca leaves for treating cancers. The ethanolic extract of Simarouba glacua leaf reported to contain secondary metabolites such as phenolic compounds, and exhibit potent anticancer activity against bladder cancer (Puranik et al, 2017). In addition, in a recent review, we have addressed the complexity of phytochemicals present in Simarouba glauca and their anti-cancer potential (Jose et al, 2019).…”
Section: Discussionmentioning
confidence: 99%
“…Moreover, it is also not known whether any other type of compounds (other than bitter substances) do present in SG leaf extracts ( Puranik et al, 2017 ). Therefore, in this study, leaves of Simarouba glauca were extracted with solvents of increasing polarity and anti-cancer activity screen performed, which identified chloroform extract as a better source for compounds with potent anti-cancer activity ( Rivero-Cruz et al, 2005 ; Puranik et al, 2017 ). Chloroform alone or in association with methanol (2:1) has been widely used to extract short chain fatty acids and triglyceride esters ( King and Min, 1998 ).…”
Section: Discussionmentioning
confidence: 99%
“…Supporting these traditional uses, preliminary studies by National Cancer Institute, United States demonstrated that alcoholic extracts of SG inhibited the growth of cancer cells even at a dose of 25 μg/ml. 1 Very recently, a study by Puranik et al (2017) showed the anti-bladder cancer activity of ethanol extract using T-24 cell line. Similarly, a separate study isolating anticancer constituents using bio activity-guided fractionation of chloroform extract of S. glauca twigs reported the presence of six canthin-6-one type alkaloid derivatives – (1) canthin-6-one; (2) 2-methoxycanthin-6-one; (3) 9-methoxycanthin-6-one; (4) 2-hydroxycanthin-6-one; (5) 4,5-dimethoxycanthin-6-one; and (6) 4,5-dihydroxycanthin-6-one; a limonoid, melianodiol, an acyclic squalene-type triterpenoid, 14-deacetyleurylene, two coumarins – scopoletin and fraxidin, and two triglycerides – triolein and trilinolein.…”
Section: Introductionmentioning
confidence: 99%