In vitro antioxidant activity, total phenolic and flavonoid contents of ethanol extract of stem and leaf of Grewia carpinifolia

Adebiyi, Olamide Elizabeth; Olayemi, F. O.; Tan, Ninghua; Zeng, Guang‐Zhi

doi:10.1016/j.bjbas.2016.12.003

Cited by 128 publications

(96 citation statements)

References 23 publications

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“…The ability to inhibit the formation of the complex MDA-TBA on methanolic extract, ethyl acetate extract and chloroform extract of C. tuberosus can be related to the total phenolic and flavonoid content (Table 1). These results were consistent with other research that indicated the content of phenols and flavonoids in the sample could suppress the MDA on the evaluation of antioxidants with TBA (Adebiyiab et al, 2017). Some research showed that the extract of C. tuberosus also contains bioactive compounds such as ursolic acid, oleanolic acid (Nugraheni et al, 2011), maslinic acid, and phytosterol compounds may inhibit peroxidation lipids by suppressing the formation of MDA so that the condensation reaction between the MDA and TBA that form the complex of the MDA-TBA can be hindered (Khennouf et al, 2010).…”

Section: Antioxidant Activity Evaluation With Tbasupporting

confidence: 93%

Phytochemical compounds and antioxidant activity of Coleus tuberosus flesh and peel on different solvent

Nugraheni¹,

Santoso²,

Windarwati³

2018

Food Res.

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Three different solvents were used to extract the phytochemical compounds in the peel and flesh of Coleus tuberosus. The results of extraction with different solvents were compared based on the levels of phytochemicals compounds and its antioxidant activity. Extraction of bioactive compounds was done by the maceration method using methanol, ethyl acetate, and chloroform for seven days at a ratio of 1:5 (w/v). Determination of total phenolic contents and flavonoids was conducted via spectrophotometric method. Antioxidant activity was evaluated based on DPPH, peroxide value and TBA value. The results showed that the methanol solvent extracted higher total phenolic and flavonoids compounds than the ethyl acetate and chloroform. The antioxidant activity of methanol extracts was higher compared to ethyl acetate and chloroform extracts for both flesh and peel of C. tuberosus. The extract of the peel of C. tuberosus had antioxidant activity higher compared to the flesh extract with the use of methanol, ethyl acetate or chloroform as solvent. There was a positive and significant correlation between total phenolic and flavonoids content against DPPH and a negative and significant correlation with peroxide and TBA value.

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Section: Antioxidant Activity Evaluation With Tbasupporting

confidence: 93%

Phytochemical compounds and antioxidant activity of Coleus tuberosus flesh and peel on different solvent

Nugraheni¹,

Santoso²,

Windarwati³

2018

Food Res.

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“…Therefore, it may be postulated that SCE has DPPH scavenging activity by reducing the radical to corresponding hydrazine because of its hydrogen ion-donating ability. 30 The results revealed a good correlation between antiradical potent and phenolic content. Many authors have reported excellent linear correlations between antioxidant/antiradical activity tests and total phenolic content.…”

Section: Dpphmentioning

confidence: 87%

In vitro and in vivo Antioxidant, Antihemolytic and Anti-inflammatory Activities of Santolina chamaecyparissus Extracts

Djarmouni¹,

Baghiani²,

Adjadj³

et al. 2018

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Introduction: Santolina chamaecyparissus L. is a small medicinal herb, cultivated in Europe, Asia and Africa due to the antihelmintic, antiseptic, anti spasmodic, bactericidal, fungicidal, digestive and vulnerary properties. Despite this, S. chamaecyparissus aerial part extractions have not been examined for antioxidant, antihemolytic and anti-inflammatory properties. Methods: S. chamaecyparissus aerial parts were extracted with solvents of varying polarity: methanol (crude) extract (CrE) chloroform extract (CHE), ethyl acetate extract (EAE), and aqueous extract (AE). The content of total phenolics, and flavonoids in all the extracts were determined with spectrophotometric methods. Both enzymatic and non-enzymatic methods were used to evaluate the antioxidant activity of the extracts. Antioxidant activity of all the extracts were investigated using free radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl, DPPH• and 2,2'-azino-bis-3-ethyl benzthiazoline-6-sulfonic acid (ABTS) assay), capacity of the inhibition of linoleic acid peroxidation (β-carotene assay), chelation of metals (iron chelating assay) and inhibition of xanthine oxidase (XO) activity. To investigate antihemolytic activity of the extracts, the 2, 2,-azobis (2-amidinopropane) dihydrochloride (AAPH) assay was used to induce erythrocyte oxidative hemolysis. An in vivo approach was carried out on mice treated with the methanol extract at a dose of 100 mg/kg/day for 21 consecutive days, and one group was treated with vitamin C (vitamin C 50 mg/kg) as a standard drug. To determine the improvement of antioxidant potential, basic biochemical parameters were used in tissue (liver), plasma and whole blood. Phorbol 12-myristate 13-acetate (PMA)-induced ear edema was used to investigate the antiinflammatory activity of methanolic extract in mice. Results: Among all the extracts analyzed, the EAE exhibited a higher phenolic and flavonoids content (373.83 ± 0.23 mg gallic acid/g, and 61.51±7.86 mg quercetin/g respectively), followed by CE and CHE. DPPH and ABTS scavenging assays showed that EAE exhibited the highest effect in the both assays, with an IC 50 of 0.01 mg/ml and 0.0085 mg/ml respectively. All extracts moderately inhibited linoleic acid oxidation, with 57 % inhibition. The CE had a considerable chelating activity on ferrous iron (IC 50 = 0.32 mg/ml). In the enzymatic method by xanthine oxidase, results demonstrated that EAE had the highest XO inhibitory effect on both XO activity and Cyt-c reduction with IC50= 0.052±0.0003 mg/ml and 0.057±0.0006 mg/ml, respectively followed by CHE and CE. In the cellular system, CrE and CHE showed a hemolysis effect with % hemolysis (62.81% ± 1.43), (62.71% ± 1.01). However, EAE provided protection against AAPH-induced hemolysis with 53.67% ± 0.97. The in vivo assay exhibited a significant decrease (79.56%) of the content of malondialdehyde (MDA) in the liver and increased glutathione (GSH) and catalase (71.95% and 59.16% respectively). The methanol extract clearly demonstrated anti-inflammatory effects...

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“…The EDTA was taken as standard. % inhibition = Ac -As /Ac × 100 Ferric Reducing Antioxidant Assay: Ferric reducing antioxidant power of the RoPt extract was determination by the method of Adebiyi et al 17 The FRAP reagent was freshly prepared by mixing 300 mmol/L sodium acetate buffer (pH 3.6), 10 mmol/L TPTZ solution in 40 mmol/L HCl and 20 mmol/L iron (III) chloride solution in a ratio of 10:1:1 to generate FRAP reaction solution, which should be warmed to 37 °C in a water bath before use. After that, 100 μL of the diluted sample was mixed with 3 mL of the FRAP reaction solution.…”

Section: Abts Radical Cation Scavenging Activitymentioning

confidence: 99%

Untitled

2019

IJPSR

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The Phlogacanthus thyrsiflorus roots have been used by the tribal population of western Assam to treat the hepatic disorder. The present study focuses on phytochemical screening, GC-MS analysis and in-vitro antioxidant activities using different extracts. Plant total phenolics, flavonoids, reducing power assay and antioxidant activity was evaluated. DPPH, FRAP, ABTS, Metal chelating activity, and H 2 O 2 free radical scavenging activity were studied. The screening from extracts showed the presence of phenols, flavonoids, tannins, resins, terpenoids, alkaloids, glycosides, cardiac glycosides, steroids. The highest concentration of total phenolics, flavonoids, reducing power and total antioxidant activity was found in the hydro-alcoholic extract of RoPt. The results of the radical scavenging activity in 70% ethanolic extract showed better IC 50 value in DPPH (265.87 ± 17.58), ABTS (17.89 ± 1.18) and H 2 O 2 (17.89 ± 1.05) µg/ml respectively. EC 50 value of 535.16 ± 34.82 was found in 70% ethanolic against 1471.32 ± 19.77 µg/ml of acetone extract whereas the FRAP showed high FeSO 4 .7H 2 O concentration in acetone extract with 820 ± 9.1 µM/ml. The major chemical constituents that were identified in GC-MS analysis were 2R-Acetoxymethyl-1,3,3-Trimethyl-4T-(3-Methyl-2-Buten-1-YL)-1T-Cyclohexanol (71.243%) and 2,4,4-Trimethyl-3-Hydroxymethyl -5A-(3-Methyl-But-2-Enyl)-Cyclohexene (88.107%) in hydro-alcoholic and acetone extracts respectively. The study showed an enhanced phenolic and flavonoid concentration and radical scavenging activity in the 70% ethanol extracts of RoPt which are the good source of antioxidants showing potential in preventing much free radical-mediated disorder.

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In vitro antioxidant activity, total phenolic and flavonoid contents of ethanol extract of stem and leaf of Grewia carpinifolia

Cited by 128 publications

References 23 publications

Phytochemical compounds and antioxidant activity of Coleus tuberosus flesh and peel on different solvent

Phytochemical compounds and antioxidant activity of Coleus tuberosus flesh and peel on different solvent

In vitro and in vivo Antioxidant, Antihemolytic and Anti-inflammatory Activities of Santolina chamaecyparissus Extracts

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