In Vitro Antimalarial Activity of Chloroform, N-Butanol, and Ethyl Acetate Fractions of Ethanol Extracts of Carthamus Tinctorius Linn. Flowers

Abstract

Objective: This objective of this research was to study in vitro antimalarial activity of chloroform, n-butanol, and ethyl acetate fractions of ethanol extracts of Carthamus tinctorius Linn. flowers from Asteraceae family which empirically been used as traditional medication by people in South Sulawesi to heal measles. Methods:Fractionation was conducted using chloroform, n-butanol, and ethyl acetate. Determination of antimalarial activity was performed by in vitro test using the 24-well microplate and the candle-jar method. Breeding is done in a petri-dish and done aseptically. Plasmodium falciparum 3D7 culture obtained from frozen deposits in-thawing and bread from Pharmacy Laboratory of Airlangga University, Surabaya, Indonesia. Blood sample with a density of over 2000 was employed. Serial decreasing concentrations of the crude extract of chloroform, butanol, and ethyl acetate fraction were tested for antimalarial activity. The following concentrations were used; 100; 10; 1.0; 0.1; and 0.01 (mg/mL). Negative controls used dimethyl sulfoxide (DMSO) diluted in the same manner as diluting materials above test, to obtain final DMSO concentration is not more than 0.5%. Mixture and suspension test parasites (= test preparation) are then inserted into the candle-jar and incubated in a CO2 incubator at a temperature of 37°C for 48 h. After incubation for 48 h made a thin blood smear on glass object. Smear dried at room temperature, fixed with methanol, then, once dry stained with Giemsa and counted under a microscope parasitemianya with 1000 times magnification. Calculations performed on 5000's erythrocytes. Results:Results showed that chloroform and n-butanol fraction cannot inhibit parasitemia >50%, but ethyl acetate fraction can inhibit parasitemia >50% with the highest inhibition at 100 µg/mL of 94.48%. Conclusion:Ethyl acetate fraction is highly active as antimalarial with an IC 50 of 1.25 µg/mL.

POTENSI KRIM EKSTRAK ETANOL DAUN MENGKUDU (Morinda citrifolia L.) TERHADAP Staphylococcus aureus

“…Ulangi proses penyarian sekurang-kurangnya satu kali dengan jenis pelarut yang sama dan jumlah volume pelarut sebanyak setengah kali jumlah volume pelarut pada penyarian pertama (1:5). Filtrat dikumpulkan lalu diuapkan menggunakan rotary evaporator (50°C, 77 mbar) hingga diperoleh ekstrak kental (Hamsidi et al, 2018).…”

In Vitro Antimalarial and Cytotoxic Activities of Sauropus androgynus Leaves Extracts

“…The percentage of the growth inhibition of the parasites was calculated using the formula below. 27 % growth = % parasitemia -D0 % inhibition = 100% -(Xs/Xc x 100%)…”

Profil Fitokimia Ekstrak Etanol Bunga Kasumba Turate (Carthamus tinctorius L.) yang Berpotensi Sebagai Antimalaria

“…Bunga Kasumba turate (Carthamus tinctorius Linn) dari suku Asteraceae merupakan tumbuhan obat tradisional yang secara empiris digunakan masyarakat Sulawesi Selatan untuk pengobatan campak yang diberikan dengan cara diseduh dengan air panas untuk meningkatkan sistem daya tahan tubuh atau sistem imun pada pasien. Penelitian sebelumnya menunjukkan ekstrak etanol dari bunga kasumba turate (C. tinctorius L.) memiliki aktivitas antimalaria yang poten secara in vitro dengan nilai IC50 sebesar 1,06 µg/ml [8,9]. Oleh karena itu, penelitian ini bertujuan untuk mengetahui profil fitokimia yang terkandung dalam ekstrak etanol bunga kasumba turate (C. tinctorius L.) yang diperoleh dengan metode maserasi.…”