In Vitro Antifungal Activities of Sulfa Drugs against Clinical Isolates of Aspergillus and Cryptococcus Species

Abstract

In vitro susceptibilities of ten clinical isolates, including five strains of Cryptococcus neoformans var. grubii and five strains of Aspergillus fumigatus, were determined against nine sulfa drugs using a microdilution method. Among the five tested media, minimum inhibitory concentration MIC values were observed only in YNB medium: no detectable level MIC value of less than 125 g/ml was observed in the four remaining media against Cryptococcus species. Of the nine sulfa drugs, of which sulfaphenazole showed the highest antifungal activity, the MIC values for A. fumigatus and C. neoformans var. grubii were, respectively, 64 g/ml and 4-8 g/ml, suggesting high susceptibility of C. neoformans to sulfa drugs.

Different supramolecular architectures mediated by different weak interactions in the crystals of three N-aryl-2,5-dimethoxybenzenesulfonamides

“…The sulfonamide linkage plays a vital role as a key constituent in a number of biologically active molecules. Up to now, sulfonamides have been known to exhibit a wide variety of biological activities, such as antibacterial (Subhakara Reddy et al, 2012; Himel et al, 1971), antifungal (Hanafy et al, 2007), anti-inflammatory ISSN 2053ISSN -2296 # 2017 International Union of Crystallography (Kuçukguzel et al, 2013), antitumor , anticancer (Mansour et al, 2011), anti-HIV (Sahu et al, 2007) and antitubercular activities (Vora & Mehta, 2012). In recent years, extensive research has been carried out on the synthesis and evaluation of the pharmacological activities of molecules containing the sulfonamide moiety for different activities, and have been reported to be important pharmacophores (Mohan et al, 2013).…”

Cotrimoxazole enhances the in vitro susceptibility of Coccidioides posadasii to antifungals

“…In a previous work, Hanafy et al (2007) found a great variability among SMX MICs against Cryptococcus sp. The authors reported that higher MIC values were attained when susceptibility tests were performed in organic-rich media because para-aminobenzoic acid (PABA) or PABA-related compounds may be present.…”

“…The authors reported that higher MIC values were attained when susceptibility tests were performed in organic-rich media because para-aminobenzoic acid (PABA) or PABA-related compounds may be present. In this study, we tried to perform the susceptibility tests with yeast nitrogen base, as suggested by Hanafy et al (2007), but unfortunately this medium did not support the growth of C. posadasii (data not shown). Because other studies have already been performed with RPMI (Yekutiel et al 2004, Navarro-Martinez et al 2006, we decided to evaluate this medium in our experiments.…”

Influence of Different Media, Incubation Times, and Temperatures for Determining the MICs of Seven Antifungal Agents against Paracoccidioides brasiliensis by Microdilution

“…First, a previous testing assay with three different inoculum concentrations (10 3 , 10 4 , and 10 5 cells/ml) in RPMI 1640 medium was performed with 13 isolates. The use of 10 3 cells/ml is suggested by the NCCLS M27-A2 protocol, though concentrations of 10 4 and 10 5 cells/ml were used in previous P. brasiliensis MIC assay studies (15,16,18,20,21,33). Our results, which are in agreement with the results of these authors, indicated that the obtained growth and MIC readings were easier to perform when a concentration of 10 5 cells/ml was used (data not shown), and this is the inoculum size in the proposed protocol (Table 5).…”