Cotrimoxazole enhances the in vitro susceptibility of Coccidioides posadasii to antifungals

Cordeiro, Rossana de Aguiar; Astete-Medrano, Delia Jessica; Marques, Francisca Jakelyne de Farias; Andrade, Heuziwanne Tavares Leite; Neto, Lauro Vieira Perdigão; Tavares, Juliane Lira; Lima, Rita Amanda Chaves de; Patoilo, Kharla Kharolyni Nobre Rabelo; Monteiro, André Jalles; Brilhante, Raimunda Sâmia Nogueira; Rocha, Marcos Fábio Gadelha; Camargo, Zoilo Pires de; Sidrim, José Júlio Costa

doi:10.1590/s0074-02762011000800024

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…These results confirm the hypothesis that cotrimoxazole has an antifungal inhibitory, as reported previously (9). This substantiating that folic acid blockade may be a potential antifungal target for Basidiobolus species.…”

Section: Discussionsupporting

confidence: 92%

“…Sterile normal saline was added to each agar slant, and the cultures were gently scraped with cotton swabs. The suspension containing conidia and hyphae was diluted 1:10 with RPMI 1640 medium (9). The suspension was transferred to a sterile tube and allowed to settle for 5 min.…”

Section: Preparation Of Fungal Suspensions (Inocula)mentioning

confidence: 99%

“…As a result of the widespread antibiotic resistance and lack of novel antibiotic options, studies are needed to discover the possible activities of known antibiotics to act on unconventional pathogens (8). Several studies have demonstrated a good clinical outcome of the antifungal effects of "non-antifungal drugs" against true pathogenic species (9-11), for example, Trimethoprim/ sulfamethoxazole (8,9), artesunate (12), and chloramphenicol (11,13). Cotrimoxazole is one of these "non-antifungal drugs," which is widely used to treat a variety of bacterial infections.…”

Section: Introductionmentioning

confidence: 99%

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Antifungal activities of artesunate, chloramphenicol, and cotrimoxazole againstBasidiobolusspecies, the causal agents of gastrointestinal basidiobolomycosis

Al‐Qahtani¹,

Joseph

Al‐Hakami

et al. 2021

Preprint

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Basidiobolus species (n =13) isolated from human gastrointestinal basidiobolomycosis and lizards were tested against artesunate, chloramphenicol, and cotrimoxazole. The three agents exhibited inhibitory actions against Basidiobolus species comparable to the known antifungals. The combined effects of artesunate + voriconazole and cotrimoxazole + voriconazole have significant synergic effects, p = 0.003 and p = 0.021, respectively. These are promising results that enhance accelerated combined treatment of GIB in humans particularly the combination of artesunate and voriconazole.

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Section: Discussionsupporting

confidence: 92%

Section: Preparation Of Fungal Suspensions (Inocula)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antifungal activities of artesunate, chloramphenicol, and cotrimoxazole againstBasidiobolusspecies, the causal agents of gastrointestinal basidiobolomycosis

Al‐Qahtani¹,

Joseph

Al‐Hakami

et al. 2021

Preprint

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“…In recent years, several studies have shown the potential of antifungal drugs to inhibit folic acid synthesis in Candida albicans [30], Paracoccidioides brasiliensis [31], Histoplasma capsulatum [32], Coccidioides posadasii [26], and Aspergillus spp. [24].…”

Section: Discussionmentioning

confidence: 99%

“…Susceptibility testing for planktonic cells was performed in 96-well microdilution plates at 37°C for 48 h. All the isolates were tested in duplicate. For SMX/TMP and SDZ/ PYR, the minimum inhibitory concentrations (MICs) were defined as the lowest drug concentration inhibiting growth by 80 % when compared to the control well [24,26].…”

Section: Effect Of Smx/tmp and Sdz/pyr On Cryptococcus Growthmentioning

confidence: 99%

Antifolates inhibit Cryptococcus biofilms and enhance susceptibility of planktonic cells to amphotericin B

Cordeiro

Mourão

Rocha

et al. 2012

Eur J Clin Microbiol Infect Dis

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The Cryptococcus neoformans species complex contains the most important agents of fungal meningoencephalitis. Therapeutic choices are limited and issues related to toxicity and resistance to antifungals have been described. The present study evaluated the inhibitory effect of the antifolate combinations sulfamethoxazole-trimethoprim (SMX/TMP) and sulfadiazine-pyrimethamine (SDZ/PYR) against planktonic cells and biofilms of C. neoformans and C. gattii. The influence of the antifolate combinations on the amphotericin minimum inhibitory concentration (MIC) of planktonic cells was also investigated. In addition, the effect of these combinations on the cellular ergosterol content of planktonic cells was studied. Strains of C. neoformans (n = 15) and C. gattii (n = 15) obtained from environmental or clinical sources were evaluated by the broth microdilution method. SMX/TMP and SDZ/PYR showed antifungal activity against free living cells and sessile cells of Cryptococcus spp. Moreover, planktonic cells showed increased susceptibility to amphotericin B after pre-incubation with sub-inhibitory concentrations of SMX/TMP or SDZ/PYR. The drug combinations SMX/TMP and SDZ/PYR were able to prevent the biofilm formation and showed inhibitory effect against mature biofilms of both species. Additionally, the study showed that antifolate drugs reduced the ergosterol content in C. neoformans and C. gattii planktonic cells. Our results highlight the antifungal potential of antifolate drugs.

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Recent developments of metal-based compounds against fungal pathogens

et al. 2021

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Cotrimoxazole enhances the in vitro susceptibility of Coccidioides posadasii to antifungals

Cited by 6 publications

References 16 publications

Antifungal activities of artesunate, chloramphenicol, and cotrimoxazole againstBasidiobolusspecies, the causal agents of gastrointestinal basidiobolomycosis

Antifungal activities of artesunate, chloramphenicol, and cotrimoxazole againstBasidiobolusspecies, the causal agents of gastrointestinal basidiobolomycosis

Antifolates inhibit Cryptococcus biofilms and enhance susceptibility of planktonic cells to amphotericin B

Recent developments of metal-based compounds against fungal pathogens

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