In vitro andin vivo degradation of poly(propylene fumarate-co-ethylene glycol) hydrogels

Suggs, Laura J.; Krishnan, R. S.; García, Celsa; Peter, Susan J.; Anderson, James M.; Mikos, Antonios G.

doi:10.1002/(sici)1097-4636(199811)42:2<312::aid-jbm17>3.0.co;2-k

Cited by 91 publications

(54 citation statements)

References 14 publications

(16 reference statements)

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“…Animals were euthanized (if necessary) with diethyl ether at specific time points (7,14,21, and 28 days) after surgery. Tissues surrounding the implanted rosin and PLGA films were sectioned, stained, and examined under a light microscope to follow the inflammatory responses.…”

Section: In Vivo Biocompatibilitymentioning

confidence: 99%

“…14 The PBS was changed every 8 hours for the first day, every day for the first week, and weekly thereafter to keep the pH relatively constant. 15 Films were withdrawn at intervals of 30, 60, and 90 days, washed with distilled water, dried, and subjected to analysis.…”

Section: In Vitro Degradationmentioning

confidence: 99%

“…This system provides a simple means by which the inflammatory exudate is monitored serially without sacrificing the animal. 27 Tissues surrounding the implanted rosin films were removed at specific postoperative points (7,14,21, and 28 days) and analyzed histopathologically for the compatibility response. The tissue in contact with rosin films evoked a moderate inflammatory response at the end of 7 days postimplantation ( Figure 4A).…”

Section: In Vivo Degradationmentioning

confidence: 99%

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Biodegradation and in vivo biocompatibility of rosin: a natural film-forming polymer

2003

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INTRODUCTIONThe specific aim of the present study was to investigate the biodegradation and biocompatibility characteristics of rosin, a natural film-forming polymer. Both in vitro as well as in vivo methods were used for assessment of the same. The in vitro degradation of rosin films was followed in pH 7.4 phosphate buffered saline at 37°C and in vivo by subdermal implantation in rats for up to 90 days. Initial biocompatibility was followed on postoperative days 7, 14, 21, and 28 by histological observations of the surrounding tissues around the implanted films. Poly (DL-lactic-co-glycolic acid) (PLGA) (50:50) was used as reference material for biocompatibility. Rate and extent of degradation were followed in terms of dry film weight loss, molecular weight (MW) decline, and surface morphological changes. Although the rate of in vitro degradation was slow, rosin-free films showed complete degradation between 60 and 90 days following subdermal implantation in rats. The films degraded following different rates, in vitro and in vivo, but the mechanism followed was primarily bulk degradation. Rosin films demonstrated inflammatory reactions similar to PLGA, indicative of good biocompatibility. Good biocompatibility comparable to PLGA is demonstrated by the absence of necrosis or abscess formation in the surrounding tissues. The study provides valuable insight, which may lead to new applications of rosin in the field of drug delivery.

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Section: In Vivo Biocompatibilitymentioning

confidence: 99%

Section: In Vitro Degradationmentioning

confidence: 99%

Section: In Vivo Degradationmentioning

confidence: 99%

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Biodegradation and in vivo biocompatibility of rosin: a natural film-forming polymer

2003

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“…A v ariety of injectable materials, both ceramic-and polymer-based, have been developed for use in multiple orthopaedic applications [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. The combination of ceramic particles with polymeric matrices has also been extensiv ely inv estigated, in an attempt to mimic bone tissue, which may itself be seen as a complex composite material made of organic and inorganic components.…”

Section: Introductionmentioning

confidence: 99%

“…The combination of ceramic particles with polymeric matrices has also been extensiv ely inv estigated, in an attempt to mimic bone tissue, which may itself be seen as a complex composite material made of organic and inorganic components. Different ceramic phases have been used, hydroxyapatite and tricalcium phosphate being the most common [4][5][6][7][8][9], as well as several polymeric matrices, both from synthetic [10][11][12][13][14] or natural origin, the latter including collagen, chitosan, gelatine and alginate, among others [15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Calcium phosphate-alginate microspheres as enzyme delivery matrices

2004

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The present study concerns the preparation and initial characterisation of nov el calcium titanium phosphate-alginate (CTPalginate) and hydroxyapatite-alginate (HAp-alginate) microspheres, which are intended to be used as enzyme delivery matrices and bone regeneration templates. Microspheres were prepared using different concentrations of polymer solution (1% and 3% w/v) and different ceramic-to-polymer solution ratios (0.1, 0.2 and 0.4 w/w). Ceramic powders were characterised using X-ray diffraction, laser granulometry, Brunauer, Emmel and Teller (BET) method for the determination of surface area, zeta potential and Fourier transform infrared spectroscopy (FT-IR). Alginate was characterised using high performance size exclusion chromatography. The methodology followed in this investigation enabled the preparation of homogeneous microspheres with a uniform size. Studies on the immobilisation and release of the therapeutic enzyme glucocerebrosidase, employed in the treatment of Gaucher disease, were also performed. The enzyme was incorporated into the ceramic-alginate matrix before gel formation in two different ways: preadsorbed onto the ceramic particles or dispersed in the polymeric matrix. The two strategies resulted in distinct release profiles. Slow release was obtained after adsorption of the enzyme to the ceramic powders, prior to preparation of the microspheres. An initial fast release was achiev ed when the enzyme and the ceramic particles were dispersed in the alginate solution before producing the microspheres. The latter profile is very similar to that of alginate microspheres. The different patterns of enzyme release increase the range of possible applications of the system inv estigated in this work.

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In vitro cytotoxicity andin vivo biocompatibility of poly(propylene fumarate-co-ethylene glycol) hydrogels

Suggs

Shive

García

et al. 1999

J. Biomed. Mater. Res.

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121

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The in vitro cytotoxicity and in vivo biocompatibility of poly(propylene fumarate-co-ethylene glycol) [P(PF-co-EG)] hydrogels were assessed in order to investigate the influence of poly(ethylene glycol) molecular weight and copolymer composition. These materials have application as injectable cardiovascular implants; cytotoxicity due to leachable products, as well as inflammation caused by the biomaterial itself, may ultimately affect the biocompatibility of the implant. We utilized a 7-day in vitro cytotoxicity assay to quantify cell density and cellular proliferation in the presence of copolymer films. The copolymer films exhibited slight to moderate cytotoxicity toward cultured endothelial cells, showing 20-86% viability relative to controls. Cell viability increased with an increasing weight percent of PEG or, to a lesser extent, the molecular weight of PEG. In vivo biocompatibility was assessed using a cage implantation model over a 21-day time period. This system was used to characterize the local cellular and humoral inflammatory response in the surrounding exudate, as well as the size and density of macrophages adherent to the material itself. All copolymer formulations exhibited excellent biocompatibility relative to controls with no significant differences in total leukocyte count among the different formulations. The in vivo inflammatory reaction displayed normal wound healing over 21 days as shown by a progressive decrease in both leukocyte concentration and enzymatic activity. The surface coverage of the copolymer films remained relatively constant from 7 to 21 days. There were no cells larger than 0.003 mm2, which was previously shown to be the threshold value for foreign-body giant cells. These data suggest that P(PF-co-EG) hydrogels have potential for use as injectable biomaterials.

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In vitro andin vivo degradation of poly(propylene fumarate-co-ethylene glycol) hydrogels

Cited by 91 publications

References 14 publications

Biodegradation and in vivo biocompatibility of rosin: a natural film-forming polymer

Biodegradation and in vivo biocompatibility of rosin: a natural film-forming polymer

Calcium phosphate-alginate microspheres as enzyme delivery matrices

In vitro cytotoxicity andin vivo biocompatibility of poly(propylene fumarate-co-ethylene glycol) hydrogels

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