In vitro andin vivo application of PLGA nanofiber for artificial blood vessel

Abstract

Poly(lactic-co-glycolic acid) (PLGA) tubes (5 mm in diameter) were fabricated using an electro spinning method and used as a scaffold for artificial blood vessels through the hybridization of smooth muscle cells (SMCs) and endothelial cells (ECs) differentiated from canine bone marrow under previously reported conditions. The potential clinical applications of these artificial blood vessels were investigated using a canine model. From the results, the tubular-type PLGA scaffolds for artificial blood vessels showed good mechanical strength, and the duallayered blood vessels showed acceptable hybridization behavior with ECs and SMCs. The artificial blood vessels were implanted and substituted for an artery in an adult dog over a 3-week period. The hybridized blood vessels showed neointimal formation with good patency. However, the control vessel (unhybridized vessel) was occluded during the early stages of implantation. These results suggest a shortcut for the development of small diameter, tubular-type, nanofiber blood vessels using a biodegradable material (PLGA).

Poly(lactic-co-glycolic) Acid/Nanohydroxyapatite Scaffold Containing Chitosan Microspheres with Adrenomedullin Delivery for Modulation Activity of Osteoblasts and Vascular Endothelial Cells

“…The superior hydrophilicity of the PLGA/nHA/CM scaffolds might easily facilitate cell migration into the pores following blood immersion in vivo . As previously observed, the hydrophilic characteristics of the scaffolds can enhance cell adhesion, migration, and proliferation in vivo compared with hydrophobic scaffolds [48]. …”

Thermally induced mesophase development in ethanesilica filmsvia macromolecular templating approach

“…The weight fraction of PEO blocks ( f PEO ) for the triblock copolymer prepared in the present study was found to be 0.38. [31][32][33][34][35] Preparation of Mesoporous Organosilica as Thin Film Form. Mesoporous organosilicate thin films were prepared using a silica sol solution containing PEO-PLGA-PEO (EO 16 Thin Film Characterization.…”

Biological, Chemical, and Electronic Applications of Nanofibers

“…Moreover, it reduced neointima formation until the end point of 6‐month study. MSCs were also seeded on nanofibers prior to implantation to prevent thrombosis 175, 181, 184. The combination of PLLA nanofibers with MSCs181 created a vascular graft with excellent patency and unique antithrombogenic property for the treatment of small‐diameter arteries.…”