In vitro and in vivo research on using Antheraea pernyi silk fibroin as tissue engineering tendon scaffolds

Qian, Fang; Chen, Denglong; Yang, Zhi‐Ming; Li, Min

doi:10.1016/j.msec.2008.12.007

Cited by 88 publications

(61 citation statements)

References 23 publications

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“…Further, A. pernyi SF also has abundant alkaline amino acids (Arg and His) and the tripeptide sequence Arg-Gly-Asp (RGD), which is known to be a cell integrin receptor and to mediate special interactions between mammalian cells. It has been demonstrated in some research that A. pernyi SF performs better in terms of cell adhesion, growth, and phenotypic maintenance than does B. mori SF [12]. Also, A. pernyi has bioproperties similar to collagen, such as cell adhesion, growth, and proliferation.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

Zhao

Xiong

et al. 2012

Advances in Materials Science and Engineering

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Silk fibroin (SF) hydrogels were obtained from both domestic (Bombyx mori) and wild (Antheraea pernyi) silkworms from aqueous silk fibroin solutions at room temperature. The gelation time of the Antheraea pernyi (A. pernyi) SF solution was significantly shorter than that of the Bombyx mori (B. mori) SF solution. The secondary structures of the two kinds of hydrogels were also compared. In order to further reduce the gelation time, various amounts of polyethylene glycol (PEG) were blended with the silk fibroins of A. pernyi and B. mori. The gelation time of both A. pernyi SF and B. mori SF decreased with the increased amount of PEG. After freeze-drying, the hydrogels were characterized through X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and Raman spectroscopy. Results showed that the addition of polyalcohol did not change the main secondary structure of the hydrogels. However, the addition of polyalcohol did reduce the gelation time and triggered additional formation of β-sheets.

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Section: Introductionmentioning

confidence: 99%

Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

Zhao

Xiong

et al. 2012

Advances in Materials Science and Engineering

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“…As results of those efforts, it was found that silk and regenerated silk had useful properties including a blood compatibility (Sakabe et al, 1989), a good cell attaching and adhesion ability (Minoura et al, 1995), a low inflammatory reaction in body (Santin et al, 1999), etc. With the findings of those properties, recently, many studies have been performed on the application of regenerated silk to biotechnological fields such as tissue engineering scaffold (Baek et al, 2008;Fang et al, 2009;Wang et al, 2006), tympanic membrane (Kim et al, 2010), wound dressing (Schneider et al, 2009), etc. In order to apply the regenerated silk to those applications, raw silk cocoon should be fabricated to fiber or film form. The raw silk cocoon is dissolved in solvent to prepare silk solution, and then the silk solution was transformed to fiber or film form by wet spinning, electrospinning, or film casting process.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Sample Handling on the Rheological Measurement of Regenerated Silk Fibroin Formic Acid Solution using Parallel Plate Geometry

Cho¹,

Um²

2011

International Journal of Industrial Entomology

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The effect of sample handling condition on the rheological measurement of regenerated silk fibroin formic acid solution using parallel plate geometry was investigated. In case of loading method, the loading by pouring sample solution resulted in the best reproducibility of rheological measurement. Loading with spoon showed a high variance of viscosity value at low shear rate region (0.01~1 sec -1 ) while loading with syringe exhibited a low reproducibility of viscosity at high shear region (1~100 sec -1 ) with a disappearance of shear thinning phenomenon. It was revealed that the sample loading with small extra amount lead to the most reproducible result. The sample loading with the exact amount for the measuring plate resulted in a lack of reproducibility of high shear viscosity, while the loading with large extra volume produced a limited consistency of low shear viscosity. It was turned out that 3 min. of waiting time before measurement was the optimum condition for reliable result. When the waiting time was less than 1 min., the low shear viscosity was obtained with a lack of consistency. On the other hand, the sample solution started drying when the waiting time increased up to 5 min.

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“…However, recent studies on nonmulberry SF have revealed its various advantages over its counter part, which include: a) improved physicochemical, thermal and mechanical properties, b) the presence of the tripeptide sequence Arg-Gly-Asp (RGD), which is the key determinant in cell-material interactions, and, c) stronger cell adhesion compared to BMSF and collagen. [3][4][5][6][7][8] These superior qualities make the non-mulberry SF a better choice than BMSF and deserving of extensive research to exploit them for biomedical applications.…”

mentioning

confidence: 99%

“…[3] Subsequently, Fang et al prepared a nonwoven scaffold by braiding the A. pernyi SF fibers and exploited its use in TE. [4] Mandal and Kundu reported the preparation of 3D scaffolds using SF isolated from silk glands of A. mylitta. [5] Subsequently, the same group reported use of A. mylitta SF-based scaffolds for stem cell-based TE.…”

mentioning

confidence: 99%

Antheraea assama Silk Fibroin‐Based Functional Scaffold with Enhanced Blood Compatibility for Tissue Engineering Applications

Kasoju

Bora

2010

Adv Eng Mater

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The architecture and surface chemistry of a scaffold determine its utility in tissue engineering (TE). Conventional techniques have limitations in fabricating a scaffold with control over both architecture and surface chemistry. To ameliorate this, in this report, we demonstrate the fabrication of an Antheraea assama silk fibroin (AASF)‐based functional scaffold. AASF is a non‐mulberry variety having superior qualities to mulberry SF and is largely unexplored in the context of TE. First, a 3D scaffold with biomimetic architecture is fabricated. The scaffold is subsequently made blood compatible by modifying the surface chemistry through a simple sulfation reaction. EDX and FTIR analysis demonstrate the successful sulfation of the scaffold. SEM observations reveal that sulfation has no any effect on the scaffold architecture. TGA reveals that it has increased thermal stability. The sulfation reaction significantly improves the overall hydrophilicity of the scaffold, as is evident from the increase in water holding capacity; this possibly enhances the blood compatibility. The enhancement in blood compatibility of the sulfated scaffold is determined from in vitro haemolysis, protein adsorption and platelet adhesion studies. The sulfated scaffold is non‐toxic and supports cell adhesion and growth, as revealed by indirect and direct contact‐based in vitro cytotoxicity assays. This study reveals that the AASF‐based functional scaffold, which has biomimetic architecture and blood‐compatible surface chemistry, could be suitable for TE applications.

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In vitro and in vivo research on using Antheraea pernyi silk fibroin as tissue engineering tendon scaffolds

Cited by 88 publications

References 23 publications

Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

The Effect of Sample Handling on the Rheological Measurement of Regenerated Silk Fibroin Formic Acid Solution using Parallel Plate Geometry

Antheraea assama Silk Fibroin‐Based Functional Scaffold with Enhanced Blood Compatibility for Tissue Engineering Applications

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