Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

Zhao, Haipeng; Xiong, Siyong; Li, Mingzhong; Zhang, Qiang; Liu, Guiyang

doi:10.1155/2012/819464

Cited by 7 publications

(5 citation statements)

References 28 publications

(36 reference statements)

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“…The β-sheet contents are associated with a more crystalline structure [51] in cryogels than the regenerated silk, which co-exists with a small amount of β-sheet and helical conformation. A similar trend in β-sheet transition is observed in A. pernyi silk fibroin when treated with polyalcohol [52]. Treatment with polyalcohol does not influence the secondary structure of the cryogels but triggers the transition.…”

Section: Discussionsupporting

confidence: 69%

Bio-inspired fabrication of fibroin cryogels from the muga silkworm Antheraea assamensis for liver tissue engineering

Kundu

Kundu²

2013

Biomed. Mater.

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Conventional scaffold fabrication techniques result in narrow pore architectures causing a limited interconnectivity and use of porogens, which affects the bio- or cyto-compatibility. To ameliorate this, cryogels are immensely explored due to their macro-porous nature, ease in fabrication, using ice crystals as porogens, the shape property, easy reproducibility and cost-effective fabrication technique. Cryogels in the present study are prepared from nonmulberry Indian muga silk gland protein fibroin of Antheraea assamensis using two different fabrication temperatures (-20 and -80 °C). Anionic surfactant sodium dodecyl sulfate is used to solubilize fibroin, which in turn facilitates gelation by accelerating the ß-sheet formation. Ethanol is employed to stabilize the 3D network and induces bimodal porosity. The gels thus formed demonstrate increased ß-sheet content (FTIR) and a considerable effect of pre-freezing temperatures on 3D micro-architectures. The cryogels are capable of absorbing large amounts of water and withstanding mechanical compression without structure deformation. Further, cell impregnated cryogels well support the viability of human hepatocarcinoma cells (live/dead assay). The formation of cellular aggregates (confocal laser and scanning electron microscope), derivation in metabolic activity and proliferation rate are obtained in constructs fabricated at different temperatures. In summary, the present work reveals promising insights in the development of a biomimetic functional template for biomedical therapeutics and liver tissue engineering.

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Section: Discussionsupporting

confidence: 69%

Bio-inspired fabrication of fibroin cryogels from the muga silkworm Antheraea assamensis for liver tissue engineering

Kundu

Kundu²

2013

Biomed. Mater.

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“…Alternatively, high stability of multilayers could be achieved at physiological temperature by covalent cross-linking of the bilayers, as it was reported for multilayers of cationized and anionic gelatin using transglutaminase . Unlike other polyelectrolytes, silk ionomers can also be cross-linked covalently by horseradish peroxidase (HRP) or Fenton reaction-mediated dityrosine bridging in the presence of low concentrations of hydrogen peroxide (H 2 O 2 ) or physically through the self-assembly of hydrophobic domains into crystalline β-sheets in the presence of polyalcohols such as PVA, PEG, or glycerol . Dissociation of silk nanocoatings from the cell surface could be delayed through covalent or physical cross-linking of ionomer bilayers, which will be investigated in our future studies.…”

Section: Results and Discussionmentioning

confidence: 99%

Synthesis and Characterization of Silk Ionomers for Layer-by-Layer Electrostatic Deposition on Individual Mammalian Cells

2020

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Nanocoating of individual mammalian cells with polymer layers has been of increasing interest in biotechnology and biomedical engineering applications. Electrostatic layer-by-layer (LbL) deposition of polyelectrolytes on negatively charged cell surfaces has been utilized for cell nanocoatings using synthetic or natural polymers with a net charge at physiological conditions. Here, our previous synthesis of silk-based ionomers through modification of silk fibroin (SF) with polyglutamate (PG) and polylysine (PL) was exploited for the nanocoating of mammalian cells. SF-PL constructs were cytotoxic to mammalian cells, thus an alternative approach for the synthesis of silk ionomers through carboxylation and amination of regenerated SF chains was utilized. Through the optimization of material properties and composition of incubation buffers, silk ionomers could be electrostatically assembled on the surface of murine fibroblasts and human mesenchymal stem cells (hMSCs) to form nanoscale multilayers without significantly impairing cell viability. The resulting silk-based protein nanoshells were transient and degraded over time, allowing for cell proliferation. The strategies presented here provide a basis for the cytocompatible nanoencapsulation of mammalian cells within silk-based artificial cell walls, with potential benefits for future studies on surface engineering of mammalian cells, as well as for utility in cell therapies, 3D printing, and preservation.

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“…There are few reports on the animal-mediated synthesis of MNPs. The most studied case is the silk fibroin, synthesized by arachnids and worms, such as Bombyx mori, which have a little catalytic and molecular recognition, so that they can be used as suture material [40,139,[169][170][171][172].…”

Section: Animal World and Synthesis Of Metallic Nanoparticlementioning

confidence: 99%

Biological synthesis of metallic nanoparticles: plants, animals and microbial aspects

Das

Pachapur

Lonappan

et al. 2017

Nanotechnol. Environ. Eng.

375

138

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The green synthesis (GS) of different metallic nanoparticles (MNPs) has re-evaluated plants, animals and microorganisms for their natural potential to reduce metallic ions into neutral atoms at no expense of toxic and hazardous chemicals. Contrary to chemically synthesized MNPs, GS offers advantages of enhanced biocompatibility and thus has better scope for biomedical applications. Plant, animals and microorganisms belonging to lower and higher taxonomic groups have been experimented for GS of MNPs, such as gold (Au), silver (Ag), copper oxide (CuO), zinc oxide (ZnO), iron (Fe 2 O 3 ), palladium (Pd), platinum (Pt), nickel oxide (NiO) and magnesium oxide (MgO). Among the different plant groups used for GS, angiosperms and algae have been explored the most with great success. GS with animal-derived biomaterials, such as chitin, silk (sericin, fibroin and spider silk) or cell extract of invertebrates have also been reported. Gram positive and gram negative bacteria, different fungal species and virus particles have also shown their abilities in the reduction of metal ions. However, not a thumb rule, most of the reducing agents sourced from living world also act as capping agents and render MNPs less toxic or more biocompatible. The most unexplored area so far in GS is the mechanism studies for different natural reducing agents expect for few of them, such as tea and neem plants. This review encompasses the recent advances in the GS of MNPs using plants, animals and microorganisms and analyzes the key points and further discusses the pros and cons of GS in respect of chemical synthesis.

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Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

Cited by 7 publications

References 28 publications

Bio-inspired fabrication of fibroin cryogels from the muga silkworm Antheraea assamensis for liver tissue engineering

Bio-inspired fabrication of fibroin cryogels from the muga silkworm Antheraea assamensis for liver tissue engineering

Synthesis and Characterization of Silk Ionomers for Layer-by-Layer Electrostatic Deposition on Individual Mammalian Cells

Biological synthesis of metallic nanoparticles: plants, animals and microbial aspects

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