In vitro and in vivo characterization of the properties of a multifiber carbon electrode allowing long-term electrochemical detection of dopamine in freely moving animals

Ganouni, Soumaya El; Forni, Claude; Nieoullon, A.

doi:10.1016/0006-8993(87)91375-8

Cited by 35 publications

(8 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As previously described [20,21], the amplitude of electrochemical signals recorded in DA solutions of 10 -8 to 10 -4 M, at 180 mV, ranged from 3 to 40 nA. No detectable signal was obtained from voltammograms recorded in DOPAC, AA, UA or HVA solutions of 10 -6 to 10 -3 M.…”

Section: Neurochemical Measurements Of Dopamine In Freely Moving Ratmentioning

confidence: 99%

Mechanism of Action of Nitrogen Pressure in Controlling Striatal Dopamine Level of Freely Moving Rats is Changed by Recurrent Exposures to Nitrogen Narcosis

et al. 2011

View full text Add to dashboard Cite

In rats, a single exposure to 3 MPa nitrogen induces change in motor processes, a sedative action and a decrease in dopamine release in the striatum. These changes due to a narcotic effect of nitrogen have been attributed to a decrease in glutamatergic control and the facilitation of GABAergic neurotransmission involving NMDA and GABA(A) receptors, respectively. After repeated exposure to nitrogen narcosis, a second exposure to 3 MPa increased dopamine levels suggesting a change in the control of the dopaminergic pathway. We investigated the role of the nigral NMDA and GABA(A) receptors in changes in the striatal dopamine levels. Dopamine-sensitive electrodes were implanted into the striatum under general anesthesia, together with a guide-cannula for drug injections into the SNc. Dopamine level was monitored by in vivo voltammetry. The effects of NMDA/GABA(A) receptor agonists (NMDA/muscimol) and antagonists (AP7/gabazine) on dopamine levels were investigated. Rats were exposed to 3 MPa nitrogen before and after five daily exposures to 1 MPa. After these exposures to nitrogen narcosis, gabazine, NMDA and AP7 had no effect on the nitrogen-induced increase in dopamine levels. By contrast, muscimol strongly enhanced the increase in dopamine level induced by nitrogen. Our findings suggest that repeated nitrogen exposure disrupted NMDA receptor function and decreased GABAergic input by modifying GABA(A) receptor sensitivity. These findings demonstrated a change in the mechanism of action of nitrogen at pressure.

show abstract

Section: Neurochemical Measurements Of Dopamine In Freely Moving Ratmentioning

confidence: 99%

Mechanism of Action of Nitrogen Pressure in Controlling Striatal Dopamine Level of Freely Moving Rats is Changed by Recurrent Exposures to Nitrogen Narcosis

et al. 2011

View full text Add to dashboard Cite

show abstract

“…The catecholamine electrode was fabricated using a variation of the technique of Forni and colleagues (Forni & Nieoullon, 1984;Ganouni, Forni & Nieoullon, 1987). A single 10 /sm carbon fibre (P55, Amoco Performance Products, Greenville, SC, USA) was attached to a silver wire using conductive silver paint (GC Electronics, Rockford, IL, USA).…”

Section: Catecholamine Electrodementioning

confidence: 99%

Developmental changes in hypoxia‐induced catecholamine release from rat carotid body, in vitro.

Donnelly

Doyle

1994

The Journal of Physiology

View full text Add to dashboard Cite

1. Developmental changes in free tissue catecholamine levels were studied using Nafion‐coated, carbon fibre electrodes placed in rat carotid bodies, in vitro. Simultaneously, single fibre chemoreceptor afferent activity was recorded from the sinus nerve. Five age groups were examined: 1, 2, 6, 10 and 20‐30 days of age. 2. Using fast‐scan voltammetry, similar current peaks were observed during exposure to exogenous dopamine and during superfusion with hypoxic saline. This suggests that changes in carbon fibre electrode current are due to an increase in free tissue catecholamines. 3. Baseline catecholamine levels were significantly less in the 1‐6 day age groups compared to 10 day and 20‐30 day rats. 4. During 1 min of hypoxia the peak concentration of tissue catecholamine was significantly less in the 1 day compared to the 2 day age groups, and these were less than in 10 day and 20‐30 day rats. 5. Peak nerve response during hypoxia increased with age from 4.5 +/‐ 0.6 Hz in the 1 day to 10.5 +/‐ 1.6 Hz in the 6 day and to 15.5 +/‐ 2.2 Hz in the 20‐30 day rats. 6. We conclude that (1) resting free tissue catecholamine levels increase with age in the newborn period, (2) hypoxia causes enhanced catecholamine release, and (3) the magnitude of the release increases in the postnatal period as does the nerve activity.

show abstract

“…We used acutely implanted microelectrodes that could be removed and calibrated after in vivo use to maintain the quantitative nature of the voltammetric approach. This strategy differs from most previous attempts with chronic voltammetric recording (Conti et al, 1978;O'Neill et al, 1982O'Neill et al, , 1983Keller et al, 1983;Dc Simoni et al, 1985;Ganouni et al, 1987;Meuller, 1987;Yamamoto and Spanos, 1988;Gratton and Wise, 1994; but also see Louillot et al, 1987;Mas et a!., 1990) and avoids complications of gliosis that may isolate the electrode from the extracellular space (Sharp and Zetterstrom, 1992). To accomplish our aim, a detachable micromanipulator mounted directly on the rat's head (Rebec et at., 1993) was used for placement of the microelectrode.…”

mentioning

confidence: 96%

Real‐Time Measurement of Electrically Evoked Extracellular Dopamine in the Striatum of Freely Moving Rats

Garris

Christensen

Rebec

et al. 1997

Journal of Neurochemistry

172

116

View full text Add to dashboard Cite

The real‐time measurement of electrically evoked dopamine was established in brain extracellular fluid of freely moving rats. Dopamine was monitored by fast‐scan cyclic voltammetry at carbon fiber microelectrodes lowered into the striatum by means of a detachable micromanipulator. A stimulating electrode, previously implanted in the substantia nigra, was used to evoke striatal dopamine efflux. Evoked extracellular dopamine was both current and frequency dependent. When low current intensities (±125 µA) and frequencies (10–20 Hz) were applied, detectable levels of dopamine were elicited without a perceptible behavioral response. Reproducible concentrations of extracellular dopamine could be evoked in the same rat for at least 2 months. These concentrations, moreover, were significantly higher in freely moving rats compared with rats anesthetized with Equithesin. Analysis of measured curves for dopamine uptake and release rates revealed that anesthesia inhibits release but does not affect uptake. It is concluded that (a) fast‐scan cyclic voltammetry at carbon fiber microelectrodes is a viable technique for the measurement of electrically evoked dopamine in brain extracellular fluid of freely moving rats, (b) it is possible to determine in situ rate constants for dopamine release and uptake from these temporally and spatially resolved measurements of levels of dopamine, and (c) transient changes in extracellular dopamine levels elicited by electrical stimulation are affected by anesthesia.

show abstract

In vitro and in vivo characterization of the properties of a multifiber carbon electrode allowing long-term electrochemical detection of dopamine in freely moving animals

Cited by 35 publications

References 22 publications

Mechanism of Action of Nitrogen Pressure in Controlling Striatal Dopamine Level of Freely Moving Rats is Changed by Recurrent Exposures to Nitrogen Narcosis

Mechanism of Action of Nitrogen Pressure in Controlling Striatal Dopamine Level of Freely Moving Rats is Changed by Recurrent Exposures to Nitrogen Narcosis

Developmental changes in hypoxia‐induced catecholamine release from rat carotid body, in vitro.

Real‐Time Measurement of Electrically Evoked Extracellular Dopamine in the Striatum of Freely Moving Rats

Contact Info

Product

Resources

About