In vitro and in vivo evaluation of linezolid loaded electrospun PLGA and PLGA/PCL fiber mats for prophylaxis and treatment of MRSA induced prosthetic infections

Böncü, Tuğba Eren; Güçlü, Aylin Üsküdar; Çatma, Mehmet Faruk; Savaşer, Ayhan; Gökçe, Aysun; Özdemir, Nurten

doi:10.1016/j.ijpharm.2019.118758

Cited by 39 publications

(26 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Entrapment efficiency was 81%, 90.2%, and 96.8% for NF2, NF4, and NF6, respectively, with 5% w/w contents of Mox. Beyond this percentage, excessive drug loading led to aggregation and undissolved parts in the polymeric solution consequently hindered their encapsulation efficiency [54], and the present results match well with similar previous results elsewhere [62,63]. High loading content was achieved with the presence of NF6 due to the event of inclusion complex property inside the cavity of β-CD which induced remarkable differentiation in the crystallinity pattern of the guest molecule [40].…”

Section: The Entrapment Efficiency Of Nanofibrous Matssupporting

confidence: 89%

See 1 more Smart Citation

Synthesis of Biocompatible and Environmentally Nanofibrous Mats Loaded with Moxifloxacin as a Model Drug for Biomedical Applications

et al. 2020

View full text Add to dashboard Cite

Biopolymeric chitosan structure (Cs) is rationally investigated owing to its potentiality in pharmaceutical applications. The synthetic routes of biomimetic Cs-based blend electrospun nanofibers were studied. Herein, biocompatible crosslinked electrospun polyvinyl alcohol (PVA)/Cs-reduced gold nanoparticles (Cs(Rg))/β-CD (beta-cyclodextrin) in pure water were fabricated. To this end, supportive PVA as a carrier, Cs bio modifier, and gold reductant and β-CD as smoother, inclusion guest molecule, and capping agent exhibit efficient entrapment of moxifloxacin (Mox) and consequently accelerate release. Besides, PVA/Cs(Rg)/β-CD paves towards controlled drug encapsulation-release affinity, antimicrobial, and for wound dressing. Without losing the nanofiber structure, the webs prolonged stability for particle size and release content up to 96.4%. The synergistic effect of the nanoformulation PVA/Cs(Rg)/β-CD against pathogenic bacteria, fungus, and yeast, including Staphylococcus aureus, Escherichia coli, Candida albicans, and Aspergillus niger, posed clear zones up to 53 φmm. Furthermore, a certain combination of PVA/Cs (Rg)/β-CD showed a total antioxidant capacity of 311.10 ± 2.86 mg AAE/g sample. In vitro cytotoxicity assay of HePG2 and MCF-7 NF6 can eradicate 34.8 and 29.3 µg/mL against selected cells.

show abstract

Section: The Entrapment Efficiency Of Nanofibrous Matssupporting

confidence: 89%

“…Indeed, b-CD in water decreased the surface tension, and this criterion imposed that b-CD acted as a surfactant [41]. As a result, a more stable solution was produced from the jet emitted from the Taylor cone, preventing such droplets, and these results matched with previous papers [52][53][54].…”

Section: Solution Evaluation Analysissupporting

confidence: 85%

Synthesis of Biocompatible and Environmentally Nanofibrous Mats Loaded with Moxifloxacin as a Model Drug for Biomedical Applications

et al. 2020

View full text Add to dashboard Cite

show abstract

“…These obtained values did not meet the expected nanofiber diameter increase that would have been achieved in correlation with the PLGA percentage increase. The increase in nanofiber diameter with the addition of PLGA can be explained by the higher molecular weight of PLGA than that of PLA since the increase in polymer molecular weight increases the viscosity, causing an increase in nanofiber diameter [ 14 – 16 ]. In other studies conducted on PLA/PLGA nanofibers, it was found that an increase in the viscosity of the solutions caused an increase in nanofiber diameters [ 8 ].…”

Section: Resultsmentioning

confidence: 99%

Effects of drug concentration and PLGA addition on the properties of electrospun ampicillin trihydrate-loaded PLA nanofibers

Böncü¹,

Özdemir²

2022

Beilstein J. Nanotechnol.

Self Cite

View full text Add to dashboard Cite

The aim of this study was to produce ampicillin trihydrate-loaded poly(lactic acid) (PLA) and PLA/poly(lactic-co-glycolic acid) (PLA/PLGA) polymeric nanofibers via electrospinning using 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) as the solvent for local application in tissue engineering. The effects of ampicillin trihydrate concentration (4–12%) and addition of PLGA (20–80%) on the spinnability of the solutions, morphology, average nanofiber diameter, encapsulation efficiency, drug release, and mechanical properties of PLA and PLA/PLGA nanofibers were examined. All nanofibers were bead-free and uniform. They had favorable encapsulation efficiency (approx. 90%) and mechanical properties. The increase in the amount of ampicillin trihydrate caused an increase in the diameter and burst effect of the nanofibers. The drug release ended on the 7th and 3rd day with nanofibers containing 4% and 12% of drug, respectively. The prolonged and controlled drug release for ten days was obtained with nanofibers containing 8% of drug. Thus, the ideal drug concentration was determined to be 8%. Nanofibers containing PLA/PLGA had a larger diameter than those including PLA. In addition, both the strength and elongation of nanofibers decreased depending on the increase in nanofiber size with the addition of PLGA, increased amount of drug, and ratios of PLGA. Drug release studies showed that PLA/PLGA nanofibers exhibited a lower burst effect and a decrease in drug release when compared to PLA nanofibers. Finally, PLA/PLGA nanofibers can be produced with enhanced encapsulation efficiency and mechanical properties, resulting in controlled and tailored release of ampicillin trihydrate for at least ten days. In conclusion, it was demonstrated that the addition of PLGA in different ratios and the amount of drug can be manipulated to obtain the desired properties (average nanofiber diameter, morphology, in vitro drug release, and mechanical properties) of PLA nanofibers.

show abstract

“…PLGA and PLGA/PCL electrospun fibers loaded with LNZ were recently evaluated for the treatment of MRSA associated with bone infections, prosthetic infections and soft tissues [ 55 ]. The nanofiber formulation, allowing a local application of LNZ, reduced the systemic side effects and guaranteed a sustained release and a prolonged antibacterial activity [ 56 ].…”

Section: Biological Activity Of Oxazolidinones and Delivery Systemsmentioning

confidence: 99%

Oxazolidinone Antibiotics: Chemical, Biological and Analytical Aspects

et al. 2021

View full text Add to dashboard Cite

This review covers the main aspects concerning the chemistry, the biological activity and the analytical determination of oxazolidinones, the only new class of synthetic antibiotics advanced in clinical use over the past 50 years. They are characterized by a chemical structure including the oxazolidone ring with the S configuration of substituent at C5, the acylaminomethyl group linked to C5 and the N-aryl substituent. The synthesis of oxazolidinones has gained increasing interest due to their unique mechanism of action that assures high antibiotic efficiency and low susceptibility to resistance mechanisms. Here, the main features of oxazolidinone antibiotics licensed or under development, such as Linezolid, Sutezolid, Eperezolid, Radezolid, Contezolid, Posizolid, Tedizolid, Delpazolid and TBI-223, are discussed. As they are protein synthesis inhibitors active against a wide spectrum of multidrug-resistant Gram-positive bacteria, their biological activity is carefully analyzed, together with the drug delivery systems recently developed to overcome the poor oxazolidinone water solubility. Finally, the most employed analytical techniques for oxazolidinone determination in different matrices, such as biological fluids, tissues, drugs and natural waters, are reviewed. Most are based on HPLC (High Performance Liquid Chromatography) coupled with UV-Vis or mass spectrometer detectors, but, to a lesser extent are also based on spectrofluorimetry or voltammetry.

show abstract

In vitro and in vivo evaluation of linezolid loaded electrospun PLGA and PLGA/PCL fiber mats for prophylaxis and treatment of MRSA induced prosthetic infections

Cited by 39 publications

References 46 publications

Synthesis of Biocompatible and Environmentally Nanofibrous Mats Loaded with Moxifloxacin as a Model Drug for Biomedical Applications

Synthesis of Biocompatible and Environmentally Nanofibrous Mats Loaded with Moxifloxacin as a Model Drug for Biomedical Applications

Effects of drug concentration and PLGA addition on the properties of electrospun ampicillin trihydrate-loaded PLA nanofibers

Oxazolidinone Antibiotics: Chemical, Biological and Analytical Aspects

Contact Info

Product

Resources

About