Effects of drug concentration and PLGA addition on the properties of electrospun ampicillin trihydrate-loaded PLA nanofibers

Abstract

The aim of this study was to produce ampicillin trihydrate-loaded poly(lactic acid) (PLA) and PLA/poly(lactic-co-glycolic acid) (PLA/PLGA) polymeric nanofibers via electrospinning using 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) as the solvent for local application in tissue engineering. The effects of ampicillin trihydrate concentration (4–12%) and addition of PLGA (20–80%) on the spinnability of the solutions, morphology, average nanofiber diameter, encapsulation efficiency, drug release, and mechanical properties of PLA and PLA/PLGA nanofibers were examined. All nanofibers were bead-free and uniform. They had favorable encapsulation efficiency (approx. 90%) and mechanical properties. The increase in the amount of ampicillin trihydrate caused an increase in the diameter and burst effect of the nanofibers. The drug release ended on the 7th and 3rd day with nanofibers containing 4% and 12% of drug, respectively. The prolonged and controlled drug release for ten days was obtained with nanofibers containing 8% of drug. Thus, the ideal drug concentration was determined to be 8%. Nanofibers containing PLA/PLGA had a larger diameter than those including PLA. In addition, both the strength and elongation of nanofibers decreased depending on the increase in nanofiber size with the addition of PLGA, increased amount of drug, and ratios of PLGA. Drug release studies showed that PLA/PLGA nanofibers exhibited a lower burst effect and a decrease in drug release when compared to PLA nanofibers. Finally, PLA/PLGA nanofibers can be produced with enhanced encapsulation efficiency and mechanical properties, resulting in controlled and tailored release of ampicillin trihydrate for at least ten days. In conclusion, it was demonstrated that the addition of PLGA in different ratios and the amount of drug can be manipulated to obtain the desired properties (average nanofiber diameter, morphology, in vitro drug release, and mechanical properties) of PLA nanofibers.

Investigation of the Real-Time Release of Doxycycline from PLA-Based Nanofibers

“…Throughout the 12 h experiment, the dissolution profiles showed sustained release, with no case exceeding 20% of the total Doxy amount released. The sustained drug release from PLA-based nanofibers is consistent with findings reported in the literature [ 53 , 54 ]. Previous research has proven the possibility of using this type of fiber for the prolonged release of drugs or biomolecules [ 55 , 56 ].…”

Revisiting the Pharmacological Potential of 3‐Hydroxyflavone Derivatives (3HF) in Transdermal Drug Delivery by Electrospun Cellulose Acetate Nanofiber

“…It was observed that the incorporation of 3HF(B) led to a decrease in the average nanofiber diameter, typically ranging from 114±20 nm for pristine nanofibers to about 80±40 nm for drug‐loaded nanofiber. This could be attributed to the interaction between the polymer and drug molecule through hydrogen bonding which in turn increased the crystallinity of the nanofiber mat [28,29] …”

“…This could be attributed to the interaction between the polymer and drug molecule through hydrogen bonding which in turn increased the crystallinity of the nanofiber mat. [28,29]…”

Fabrication and Optimization of Electrospun Shellac Fibers Loaded with Senna alata Leaf Extract

“…Conversely, as the concentration of both shellac and extract increased, a significant reduction in the efficiency of entrapment was detected. The potential cause of this decrease could be the existence of immobile constituents in the solution or the substances’ poor solubility [ 70 ]. These findings provide valuable insights into the interplay between solution composition and entrapment efficiency, offering guidance for optimizing electrospinning conditions to achieve effective incorporation of components within the fibers for various applications in materials science.…”