In Vitro Activity of Caffeic Acid Phenethyl Ester against Different Oral Microorganisms

Abstract

This was an in vitro study that aimed to evaluate the antimicrobial effect of the propolis extract caffeic acid phenethyl ester (CAPE) on four different oral microorganisms. Seven different concentrations of CAPE (0.2, 0.5, 1, 1.5, 2, 3, and 4 mg/mL) for use against Staphylococcus aureus, Streptococcus mutans, Streptococcus oralis, and Streptococcus salivarius were determined using minimum inhibition concentration (MIC), minimum bactericidal concentration (MBC), broth microdilution, and well diffusion tests over 1, 3, 6, 12, and 24 h, while NaF at 0.05 percent was used as a positive control. Staphylococcus aureus was most affected by CAPE’s inhibitory effect on bacterial growth, whereas S. mutans was the least affected. S. mutans and S. oralis had similar CAPE MIC and MBC values of 1 mg/mL and 1.5 mg/mL, respectively. The most resistant bacteria to CAPE were S. salivarius and S. aureus, with MIC and MBC values of 3 mg/mL and 4 mg/mL, respectively. S. oralis, followed by S. salivarius, S. mutans, and S. aureus, had the highest viable count following exposure to CAPE’s MBC values, while S. aureus had the lowest. The current results of the inhibitory effect of CAPE on bacterial growth are promising, and the values of both CAPE MBC and MIC against the related four cariogenic bacterial organisms are significant. CAPE can be employed as an adjunct dental hygiene substance for maintaining good oral hygiene, and has a potential therapeutic effect in the field of oral health care.

Host–Guest Interactions of Caffeic Acid Phenethyl Ester with β-Cyclodextrins: Preparation, Characterization, and In Vitro Antioxidant and Antibacterial Activity

“…AlSheikh et al (2022) found 3 mg/mL MIC on S. aureus using commercial CAPE and determined S. aureus as the most resistant among their test organisms as well as it has dose-dependent bactericidal action. 48 In the study of Kishimoto et al (2005), 10 CAPE inhibited bacterial growth of S. aureus with 0.22–0.44 mM MIC value but not showed any antimicrobial activity on E. coli as well as like the other several studies 6 , 49 contrary to our findings.…”

“…aureus using commercial CAPE and determined S. aureus as the most resistant among their test organisms as well as it has dose-dependent bactericidal action . In the study of Kishimoto et al (2005), CAPE inhibited bacterial growth of S.…”

Influence of Propolis Extract (Caffeic Acid Phenethyl Ester) Addition on the Candida albicans Adhesion and Surface Properties of Autopolymerized Acrylic Resin

“…Other studies evaluated CAPE's antibacterial effects on oral cariogenic bacteria. These studies showed a remarkable antimicrobial effect on cariogenic bacteria by inhibiting biofilms formation [ 32 , 33 ].…”

Antimicrobial Activity of Biogenic Silver Nanoparticles from Syzygium aromaticum against the Five Most Common Microorganisms in the Oral Cavity

“…The oral cavity is a complex ecosystem that is inhabited by more than 300 bacterial species. Some of these have been implicated in oral diseases such as caries and periodontitis, which are among the most common bacterial infections in humans [ 42 ]. The bacteria colonize the teeth in a reasonably predictable sequence.…”