In-situ generated molecularly imprinted material for chloramphenicol electrochemical sensing in waters down to the nanomolar level

Cardoso, Ana R.; Tavares, Ana P.M.; Sales, M. Goreti F.

doi:10.1016/j.snb.2017.10.114

Cited by 60 publications

(54 citation statements)

References 21 publications

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“…There are several monomers that may be employed to electrically generate MIP materials, which are typically aromatic compounds with different functionalities [13,14,15]. In this regard, our group has pioneered the use of Eriochrome Black T (EBT) as functional monomer, which yielded very stable and controllable polymeric surfaces [16,17]. EBT has several functional groups, e.g., sulfonate, hydroxyl, nitrogen dioxide, and diazo, enabling different interactions with a given target analyte, and it is classified as an anionic acidic dye with highly extended π system.…”

Section: Introductionmentioning

confidence: 99%

An impedimetric molecularly-imprinted biosensor for Interleukin-1β determination, prepared by in-situ electropolymerization on carbon screen-printed electrodes

Cardoso

Sá

Sales

2019

Bioelectrochemistry

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This work reports the first electrochemical molecularly imprinted polymer (MIP) sensor for Interleukin-1beta (IL-1β) detection, based on modified commercial screen-printed carbon electrode (SPCE) was successfully demonstrated. For this purpose, the carbon support was modified with a PEDOT/4-aminothiophenol layer prior to the MIP film to enhance sensitivity and signal stability. The MIP layer was constructed on top of this by electropolymerization of Eriochrome black T (EBT) in the presence of IL-1β. The several steps of the biosensor assembly was followed by Raman spectroscopy and electroanalytical techniques. Using electrochemical impedance spectroscopy (EIS), a linear response in the range of 60 pM to 600 nM, with a LOD of 1.5 pM with (S/N = 3) was obtained in neutral PBS. Selectivity tests of the MIP biosensor made in spiked synthetic serum samples as well as against other structurally related (Myoglobin, of similar shape and size) or competing compounds (Immunoglobulin G, also present in the human serum) confirmed the good selectivity of the biosensor. Overall, the biosensor described herein has the potential to provide a simple and quick way for on-site screening of IL-1β, with low sample/reagent consumption and enabling direct serum analysis, which constitutes a valuable alternative to other conventional methods.

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Section: Introductionmentioning

confidence: 99%

An impedimetric molecularly-imprinted biosensor for Interleukin-1β determination, prepared by in-situ electropolymerization on carbon screen-printed electrodes

Cardoso

Sá

Sales

2019

Bioelectrochemistry

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“…MIPs are formed by a combination of template molecules with adequate functional monomers and crosslinkers. Recently, MIP assemblies have shown evidences of potential application in the detection of some biomarkers, with low limits of detection and rapid diagnosis results [14][15][16][17][18][19], but none of these works are related to IL-2 detection. Moreover, the combination of MIPs with fluorescencebased sensors for protein detection in emerging with great relevance in the field of optical sensing due to their high sensitivity and specificity, along with a fast response and simplicity of implementation.…”

Section: Introductionmentioning

confidence: 99%

Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers

Piloto

Ribeiro

Rodrigues

et al. 2020

Sensors and Actuators B: Chemical

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Herein, the development of a fluorescent-based sensor by combining quantum dots (QDs) with molecularlyimprinted technology (MIT), intensively optimized to generate exceptional operating features is presented. This sensor is designed to target human interleukin-2 (IL-2) in synthetic human serum. IL-2 is a regulatory protein released as a triggered response from the immune system towards an inflammation.For this purpose, cadmium telluride (CdTe) QDs are prepared with 3-mercaptopropionic acid (MPA) and modified afterwards to produce an IL-2 imprinted polymer with methacrylic acid and N,N´-methylenebis(acrylamide), upon removal of the template under optimized conditions. During IL-2 rebinding, the fluorescence intensity of CdTe@MPA QDs is quenched in a concentration dependent manner. Using surface imprinting technology, the optimal fluorescence signals yielded a linear response versus logarithm of IL-2 concentration from 35 fg/ml to 39 pg/ml, in a 1000-fold diluted synthetic human serum. The limit of detection obtained is 5.91 fg/ml, lying below the concentration levels of IL-2 with clinical interest for cancer diagnosis (9.4-19.2 pg/ ml).Overall, the method presented herein is a demonstration that the combination of MIP and QDs for protein detection constitutes a powerful tool in clinical analysis, providing low cost, sensitive and quick responses. The same concept may be further extended to other proteins of interest.

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“…The molecularly imprinted polymers (MIPs) have specific cavities with same structure as the template molecule in shape, size, and main functional groups, which have attracted great attention due to their benefits such as selective adsorption and chemical stability. Recently, MIT has been successfully applied in many fields, but not limited to, SPE and chemical sensors .…”

Section: Introductionmentioning

confidence: 99%

Preparation and application of simetryn‐imprinted nanoparticles in triazine herbicide residue analysis

Tong

Han

et al. 2020

J of Separation Science

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This work provides a simple and rapid method for synthesis uniform simetryn imprinted nanoparticles, which can be used to pretreat the tested samples before detecting. A series of computational approach were employed for design simetryn‐imprinted polymer. Based on the conclusion of theoretical calculation, the simetryn imprinted nanoparticles were synthesized using simetryn as template, methacrylic acid as monomer with different solvent volume and synthesis conditions. The obtained nanoparticles have small size, uniform distribution and high imprinted factor. Scatchard analysis and quantum chemical calculations were applied for evaluating the interaction of simetryn with methacrylic acid in the imprinting process. The selectivity and recognition ability of the simetryn imprinted nanoparticles for six triazine herbicides and two other type herbicides were investigated. The results show that the simetryn imprinted nanoparticles had high selectivity and binding capacity and could be used for the separation and enrichment of four triazine pesticide residues from actual samples. A method of molecularly imprinted matrix solid phase extraction ultra‐performance liquid chromatography tandem mass spectrometry was established for detecting four kinds of triazine herbicide residues in tobacco. The recovery rate of terbuthylazine, simetryn, atrazine, and prometryn in tobacco was 84.03–119.05%, and the relative standard deviation was 0.35–10.12%.

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In-situ generated molecularly imprinted material for chloramphenicol electrochemical sensing in waters down to the nanomolar level

Cited by 60 publications

References 21 publications

An impedimetric molecularly-imprinted biosensor for Interleukin-1β determination, prepared by in-situ electropolymerization on carbon screen-printed electrodes

An impedimetric molecularly-imprinted biosensor for Interleukin-1β determination, prepared by in-situ electropolymerization on carbon screen-printed electrodes

Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers

Preparation and application of simetryn‐imprinted nanoparticles in triazine herbicide residue analysis

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