An impedimetric molecularly-imprinted biosensor for Interleukin-1β determination, prepared by in-situ electropolymerization on carbon screen-printed electrodes

Abstract

This work reports the first electrochemical molecularly imprinted polymer (MIP) sensor for Interleukin-1beta (IL-1β) detection, based on modified commercial screen-printed carbon electrode (SPCE) was successfully demonstrated. For this purpose, the carbon support was modified with a PEDOT/4-aminothiophenol layer prior to the MIP film to enhance sensitivity and signal stability. The MIP layer was constructed on top of this by electropolymerization of Eriochrome black T (EBT) in the presence of IL-1β. The several steps of the biosensor assembly was followed by Raman spectroscopy and electroanalytical techniques. Using electrochemical impedance spectroscopy (EIS), a linear response in the range of 60 pM to 600 nM, with a LOD of 1.5 pM with (S/N = 3) was obtained in neutral PBS. Selectivity tests of the MIP biosensor made in spiked synthetic serum samples as well as against other structurally related (Myoglobin, of similar shape and size) or competing compounds (Immunoglobulin G, also present in the human serum) confirmed the good selectivity of the biosensor. Overall, the biosensor described herein has the potential to provide a simple and quick way for on-site screening of IL-1β, with low sample/reagent consumption and enabling direct serum analysis, which constitutes a valuable alternative to other conventional methods.

Advances in Nanotechnology-Based Biosensing of Immunoregulatory Cytokines

“…Using EIS detection, a linear response range of 60 pM to 600 nM with a detection limit of 1.5 pM was obtained. Selectivity tests confirmed good selectivity of the developed biosensor [148].…”

Electropolymerized Receptor Coatings for the Quantitative Detection of Histamine with a Catheter-Based, Diagnostic Sensor

“…This suggests that MIP materials might be better for which the histamine recognition does not rely on hydrogen bridges but, instead, on pH-independent interactions such as π-π stacking. 30 MIP materials allowing for π-π interactions can for instance be synthesized through electropolymerization from monomers with aromatic ring structures: Well-known examples include PEDOT-PSS (poly(3,4-ethylene-dioxythiophene) polystyrene sulfonate), [31][32][33] and p-ATP (poly-aminothiophenol), 34 while ref. 35 reviews all recent evolutions up to 2019.…”

“…MIP materials allowing for π–π interactions can, for instance, be synthesized through electropolymerization from monomers with aromatic ring structures: well-known examples include poly­(3,4-ethylene-dioxythiophene)–polystyrene sulfonate − and poly-aminothiophenol, while ref reviews all recent evolutions up to 2019. In the present work, we chose pyrrole (Py) (see Figure ) as a functional monomer for several reasons: Py has been used before to synthesize histamine-sensitive MIP coatings on diamond electrodes, albeit still without a cross-selectivity test toward histidine .…”

Sensor detection in gynaecological medicine

“…Another effort is an impedimetric sensor based on an electrochemical molecularly imprinted polymer for Il-1β [244]. This sensor used commercial screen-printed carbon electrodes, and provided a limit-of-detection of 1.5 pM, with a dynamic range of 60 pM -600 nM.…”