In situ formed anti-inflammatory hydrogel loading plasmid DNA encoding VEGF for burn wound healing

Wang, Peng; Huang, Shaobin; Hu, Zhicheng; Yang, Wei; Lan, Yong; Zhu, Jiayuan; Hancharou, Andrei; Guo, Rui; Tang, Bin

doi:10.1016/j.actbio.2019.10.004

Cited by 159 publications

(127 citation statements)

References 41 publications

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“…2 However, excessive inflammation is considered a major obstacle in cutaneous wound healing. 3 Long-lasting cutaneous wound healing has been reported to cause a huge financial burden to the patients whilst the healing results remain unsatisfactory. 4 To overcome the barrier of cutaneous wound healing, improvement of the new vessel growth, namely, angiogenesis, and stimulation of regenerative tissues are endorsed for subsequent research.…”

Section: Introductionmentioning

confidence: 99%

Extracellular vesicle‐enclosed miR‐486‐5p mediates wound healing with adipose‐derived stem cells by promoting angiogenesis

Wen

Jing

et al. 2020

J Cellular Molecular Medi

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Adipose‐derived stem cells (ASC) are said to have a pivotal role in wound healing. Specifically, ASC‐secreted extracellular vesicles (EV) carry diverse cargos such as microRNAs (miRNAs) to participate in the ASC‐based therapies. Considering its effects, we aimed to investigate the role of ASC‐EVs in the cutaneous wound healing accompanied with the study on the specific cargo‐medicated effects on wound healing. Two full‐thickness excisional skin wounds were created on mouse dorsum, and wound healing was recorded at the indicated time points followed by histological analysis and immunofluorescence staining for CD31 and α‐SMA. Human skin fibroblasts (HSFs) and human microvascular endothelial cells (HMECs) were co‐cultured with EVs isolated from ASC (ASC‐EVs), respectively, followed by the evaluation of their viability and mobility using CCK‐8, scratch test and transwell migration assays. Matrigel‐based angiogenesis assays were performed to evaluate vessel‐like tube formation by HMECs in vitro. ASC‐EVs accelerated the healing of full‐thickness skin wounds, increased re‐epithelialization and reduced scar thickness whilst enhanced collagen synthesis and angiogenesis in murine models. However, miR‐486‐5p antagomir abrogated the ASC‐EVs‐induced effects. Intriguingly, miR‐486‐5p was found to be highly enriched in ASC‐EVs, exhibiting an increase in viability and mobility of HSFs and HMECs and enhanced the angiogenic activities of HMECs. Notably, we also demonstrated that ASC‐EVs‐secreted miR‐486‐5p achieved the aforesaid effects through its target gene Sp5. Hence, our results suggest that miR‐486‐5p released by ASC‐EVs could be a critical mediator to develop an ASC‐based therapeutic strategy for wound healing.

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Section: Introductionmentioning

confidence: 99%

Extracellular vesicle‐enclosed miR‐486‐5p mediates wound healing with adipose‐derived stem cells by promoting angiogenesis

Wen

Jing

et al. 2020

J Cellular Molecular Medi

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“…At 16th, 24th days, promising healing was achieved with faster granulation, enhanced collagenization, patterned re-epithelialization by EGF treated cellular immobilized PU/SF in normal, hyperglycemic burn. Inflammatory cytokines and immunoglobulins like IL-6, IL-8, IL-10 were examined, suggesting the anti-inflammatory role of PU/SF [ 88 ].…”

Section: Engineered Scaffolds For Wound Healingmentioning

confidence: 99%

Scaffolds for Wound Healing Applications

2020

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In order to overcome the shortcomings related to unspecific and partially efficient conventional wound dressings, impressive efforts are oriented in the development and evaluation of new and effective platforms for wound healing applications. In situ formed wound dressings provide several advantages, including proper adaptability for wound bed microstructure and architecture, facile application, patient compliance and enhanced therapeutic effects. Natural or synthetic, composite or hybrid biomaterials represent suitable candidates for accelerated wound healing, by providing proper air and water vapor permeability, structure for macro- and microcirculation, support for cellular migration and proliferation, protection against microbial invasion and external contamination. Besides being the most promising choice for wound care applications, polymeric biomaterials (either from natural or synthetic sources) may exhibit intrinsic wound healing properties. Several nanotechnology-derived biomaterials proved great potential for wound healing applications, including micro- and nanoparticulate systems, fibrous scaffolds, and hydrogels. The present paper comprises the most recent data on modern and performant strategies for effective wound healing.

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“…The main role played by VEGF is in the process of angio- and/or vasculo-genesis, essential in wound healing, but also it contributes significantly to the improvement of collagen deposition and re-epithelialization. Hence, this subtype of growth factor will improve the vascular permeability and the angiogenesis by stimulating endothelial cell migration and dissemination and by permitting the penetration of inflammatory cells into the wound area [ 20 ]. FGFs are a large group of secretory cytokines known to possess a powerful chemo-attractant and mitogenic action towards the endothelial cells, participating in their recruitment, differentiation, and dissemination.…”

Section: Wound Healingmentioning

confidence: 99%

An Overview of Biopolymeric Electrospun Nanofibers Based on Polysaccharides for Wound Healing Management

et al. 2020

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Currently, despite the thoroughgoing scientific research carried out in the area of wound healing management, the treatment of skin injuries, regardless of etiology remains a big provocation for health care professionals. An optimal wound dressing should be nontoxic, non-adherent, non-allergenic, should also maintain a humid medium at the wound interfacing, and be easily removed without trauma. For the development of functional and bioactive dressings, they must meet different conditions such as: The ability to remove excess exudates, to allow gaseous interchange, to behave as a barrier to microbes and to external physical or chemical aggressions, and at the same time to have the capacity of promoting the process of healing by stimulating other intricate processes such as differentiation, cell adhesion, and proliferation. Over the past several years, various types of wound dressings including hydrogels, hydrocolloids, films, foams, sponges, and micro/nanofibers have been formulated, and among them, the electrospun nanofibrous mats received an increased interest from researchers due to the numerous advantages and their intrinsic properties. The drug-embedded nanofibers are the potential candidates for wound dressing application by virtue of: Superior surface area-to volume ratio, enormous porosity (can allow oxy-permeability) or reticular nano-porosity (can inhibit the microorganisms’adhesion), structural similitude to the skin extracellular matrix, and progressive electrospinning methodology, which promotes a prolonged drug release. The reason that we chose to review the formulation of electrospun nanofibers based on polysaccharides as dressings useful in wound healing was based on the ever-growing research in this field, research that highlighted many advantages of the nanofibrillary network, but also a marked versatility in terms of numerous active substances that can be incorporated for rapid and infection-free tissue regeneration. In this review, we have extensively discussed the recent advancements performed on electrospun nanofibers (eNFs) formulation methodology as wound dressings, and we focused as well on the entrapment of different active biomolecules that have been incorporated on polysaccharides-based nanofibers, highlighting those bioagents capable of improving the healing process. In addition, in vivo tests performed to support their increased efficacy were also listed, and the advantages of the polysaccharide nanofiber-based wound dressings compared to the traditional ones were emphasized.

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In situ formed anti-inflammatory hydrogel loading plasmid DNA encoding VEGF for burn wound healing

Cited by 159 publications

References 41 publications

Extracellular vesicle‐enclosed miR‐486‐5p mediates wound healing with adipose‐derived stem cells by promoting angiogenesis

Extracellular vesicle‐enclosed miR‐486‐5p mediates wound healing with adipose‐derived stem cells by promoting angiogenesis

Scaffolds for Wound Healing Applications

An Overview of Biopolymeric Electrospun Nanofibers Based on Polysaccharides for Wound Healing Management

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