In situ detection of amastigotes in American cutaneous leishmaniasis, using monoclonal antibodies

Lynch, Neil R.; Malavé, Caridad; Ifante, R.Benito; Modlin, Robert L.; Convit, J

doi:10.1016/0035-9203(86)90183-5

Cited by 19 publications

(6 citation statements)

References 16 publications

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“…However, newer immunologic and molecular techniques are now available. In situ detection of amastigotes with characterization of the infecting parasite species can be done in CL by immunocytochemical procedures, using either polyclonal (274) or monoclonal (8,178) antibodies. Rapid in situ diagnosis of leishmaniasis is also possible by examining dot blots of fluid aspirates or touch blots of infected tissues on nitrocellulose paper.…”

Section: Laboratory Diagnosismentioning

confidence: 99%

Leishmaniases of the New World: current concepts and implications for future research

1993

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Recent epidemiologic studies indicate that leishmaniasis in the Americas is far more abundant and of greater public health importance than was previously recognized. The disease in the New World is caused by a number of different parasite species that are capable of producing a wide variety of clinical manifestations. The outcome of leishmanial infection in humans is largely dependent on the immune responsiveness of the host and the virulence of the infecting parasite strain. This article reviews current concepts of the clinical forms, immunology, pathology, laboratory diagnosis, and treatment of the disease as well as aspects of its epidemiology and control. Recommendations for future research on the disease and its control are made.

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Section: Laboratory Diagnosismentioning

confidence: 99%

Leishmaniases of the New World: current concepts and implications for future research

1993

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“…More recent methods for detection of the parasite such as immunohistochemistry (IHC) [7][8][9]13,[15][16][17] and polymerase chain reaction (PCR) 6,11,12,18 have been shown to be more sensitive and are beginning to be established in the laboratory routine as auxiliary or alternative methods. Considering this detection and regarding diagnostic performance parameters (sensitivity, e.g.…”

Section: Introductionmentioning

confidence: 99%

Immunoperoxidase technique using an anti-Leishmania (L.) chagasi hyperimmune serum in the diagnosis of culture-confirmed American tegumentary leishmaniasis

Quintella

Cuzzi

Madeira

et al. 2009

Rev. Inst. Med. trop. S. Paulo

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The present study reports the production of the rabbit anti-Leishmania (L.) chagasi hyperimmune serum, the standardization of the immunohistochemistry (IHC) technique and the evaluation of its employment in cutaneous leishmaniasis (CL) lesions diagnosed by Leishmania sp. culture isolation. Thirty fragments of active CL lesions were examined as well as 10 fragments of cutaneous mycosis lesions as control group. IHC proved more sensitive in detecting amastigotes than conventional hematoxylin-eosin (HE) stained slides: the former was positive in 24 (80%) biopsies whereas the latter, in 16 (53%) (p = 0.028). The reaction stained different fungus species causing cutaneous mycosis. Besides, positive reaction was noticed in mononuclear and endothelial cells. Nevertheless, this finding was present in the control group biopsies. It is concluded that IHC showed good sensitivity in detecting amastigotes.

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“…The pattern of staining of the promastigotes in the immunoperoxidase test used indicated that a high proportion of the IgE antibody was directed against surface components of the organisms. IgE reacting with the intracellular amastigote form of the parasite was rarely detected, and this was not simply due to technical difficulties in the staining of intracellular antigens, as anti-leishmania monoclonal antibodies produced strong reactions under identical conditions (Lynch et al 1985). Differences in the antigenic composition between promastigotes and amastigotes have been described (Handman & Hocking 1982, Fong & Chang 1982, Jaffe & McMahon-Pratt 1983, and possibly promastigotes preferentially stimulate IgE antibody responses.…”

Section: Discussionmentioning

confidence: 99%

“…After a subsequent wash, the macrophage preparations were counterstained in Mayer's haematoxylin. The intensity of the immunoperoxidase staining of the parasites was judged by comparison with control slides that had been treated with anti-Leishmania monoclonal antibodies (Lynch et al 1985), and was classified as being either markedly positive, doubtful or clearly negative.…”

Section: Immunoperoxidase S T a I N I N Gmentioning

confidence: 99%

IgE antibody against surface antigens of Leishmania promastigotes in American cutaneous leishmaniasis

Lynch

Malavé

Turner

et al. 1986

Parasite Immunology

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As IgE-mediated immune mechanisms participate in the host defence against some types of parasites, we evaluated sera from American cutaneous leishmaniasis (ACL) patients for the presence of this antibody against Leishmania. Using monoclonal antibodies against human IgE and an immunoperoxidase staining technique, 48% of the patients sera tested were found to contain IgE antibody that bound strongly to Leishmania promastigotes. A much lower proportion of sera from non-symptomatic subjects from either endemic or non-endemic areas of the disease contained significant levels of anti-Leishmania IgE antibody (6.5% and 0% respectively). The results indicated that the IgE antibody bound predominantly to surface components of the promastigotes, and reactivity against the intracellular amastigote form of the parasite was rarely detected. Somewhat unexpectedly, in a small proportion of the sera, the IgE antibody showed apparent specificity for L. mexicana or L. braziliensis. This study demonstrates that ACL patients can develop anti-Leishmania IgE antibody responses, that seem to be directed preferentially against surface antigens of promastigotes, and that can be strain specific. This raises the question as to the possible contribution of this antibody to the immune defence mechanisms against the parasite.

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In situ detection of amastigotes in American cutaneous leishmaniasis, using monoclonal antibodies

Cited by 19 publications

References 16 publications

Leishmaniases of the New World: current concepts and implications for future research

Leishmaniases of the New World: current concepts and implications for future research

Immunoperoxidase technique using an anti-Leishmania (L.) chagasi hyperimmune serum in the diagnosis of culture-confirmed American tegumentary leishmaniasis

IgE antibody against surface antigens of Leishmania promastigotes in American cutaneous leishmaniasis

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