In-situ crosslinked hydrogel based on amidated pectin/oxidized chitosan as potential wound dressing for skin repairing

Amirian, Jhaleh; Zeng, Yicheng; Shekh, Mehdihasan I.; Sharma, Gaurav; Stadler, Florian J.; Song, Jun; Du, Bing; Zhu, Yanxia

doi:10.1016/j.carbpol.2020.117005

Cited by 155 publications

(75 citation statements)

References 56 publications

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“…One strategy to overcome these limitations is a modification of the pristine alginate to obtain its oxidized product alginate di-aldehyde (ADA) [ 12 ], which is then subsequently crosslinked with gelatin (GEL) in order to improve cell adhesiveness and to control the biodegradability, hydrolytic properties, as well as shape fidelity of 3D printed structures, which can be achieved by changing the content of ADA and GEL and the extent of alginate oxidation [ 10 ]. This strategy of dynamic chemical crosslinking by Schiff base chemistry represents one of the current trends reported in the literature and has also been used with other natural materials [ 13 , 14 ]. ADA with different GEL contents has been reported to have advantages in several biomedical applications such as wound dressing [ 15 ], improvement of cartilage tissue formation [ 16 ], development of cell adhesive surfaces [ 17 ] and as injectable cell delivery vehicle for adipose tissue engineering [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Differential Responses to Bioink-Induced Oxidative Stress in Endothelial Cells and Fibroblasts

Genç

Hazur

Karakaya

et al. 2021

IJMS

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A hydrogel system based on oxidized alginate covalently crosslinked with gelatin (ADA-GEL) has been utilized for different biofabrication approaches to design constructs, in which cell growth, proliferation and migration have been observed. However, cell–bioink interactions are not completely understood and the potential effects of free aldehyde groups on the living cells have not been investigated. In this study, alginate, ADA and ADA-GEL were characterized via FTIR and NMR, and their effect on cell viability was investigated. In the tested cell lines, there was a concentration-dependent effect of oxidation degree on cell viability, with the strongest cytotoxicity observed after 72 h of culture. Subsequently, primary human cells, namely fibroblasts and endothelial cells (ECs) were grown in ADA and ADA-GEL hydrogels to investigate the molecular effects of oxidized material. In ADA, an extremely strong ROS generation resulting in a rapid depletion of cellular thiols was observed in ECs, leading to rapid necrotic cell death. In contrast, less pronounced cytotoxic effects of ADA were noted on human fibroblasts. Human fibroblasts had higher cellular thiol content than primary ECs and entered apoptosis under strong oxidative stress. The presence of gelatin in the hydrogel improved the primary cell survival, likely by reducing the oxidative stress via binding to the CHO groups. Consequently, ADA-GEL was better tolerated than ADA alone. Fibroblasts were able to survive the oxidative stress in ADA-GEL and re-entered the proliferative phase. To the best of our knowledge, this is the first report that shows in detail the relationship between oxidative stress-induced intracellular processes and alginate di-aldehyde-based bioinks.

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Section: Introductionmentioning

confidence: 99%

Differential Responses to Bioink-Induced Oxidative Stress in Endothelial Cells and Fibroblasts

Genç

Hazur

Karakaya

et al. 2021

IJMS

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“…The swelling ratio and compressive modulus ( Figure 2 I,J) of the different hydrogels group did not exhibit remarkable changes, which could be due to the low concentrations of the BG. Excellent swelling ability is one of the important conditions to recover scar and promote skin wound healing by providing moist environment [ 39 ]. It is also proved that the high swelling ability enables hydrogels to enrich coagulation factors and increase bleeding rate after bleeding [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Multifunctional Injectable Hydrogel Loaded with Cerium-Containing Bioactive Glass Nanoparticles for Diabetic Wound Healing

et al. 2021

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Diabetic foot wound healing is a major clinical problem due to impaired angiogenesis and bacterial infection. Therefore, an effective regenerative dressing is desiderated with the function of promoting revascularization and anti-bacteria. Herein, a multifunctional injectable composite hydrogel was prepared by incorporation of the cerium-containing bioactive glass (Ce-BG) into Gelatin methacryloyl (GelMA) hydrogel. The Ce-BG was synthesized by combining sol-gel method with template method, which maintained spherical shape, chemical structure and phase constitution of bioactive glass (BG). The Ce-BG/GelMA hydrogels had good cytocompatibility, promoted endothelial cells migration and tube formation by releasing Si ion. In vitro antibacterial tests showed that 5 mol % CeO2-containing bioactive glass/GelMA (5/G) composite hydrogel exhibited excellent antibacterial properties. In vivo study demonstrated that the 5/G hydrogel could significantly improve wound healing in diabetic rats by accelerating the formation of granulation tissue, collagen deposition and angiogenesis. All in all, these results indicate that the 5/G hydrogel could enhance diabetic wound healing. Therefore, the development of multifunctional materials with antibacterial and angiogenic functions is of great significance to promote the repair of diabetic wound healing.

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“…After long years of industrial applications, HGs have been widely studied because of their three-dimensional networks, non-solubility in water at a physiological temperature or pH, noticeable swellability in aqueous media, and porosity formed by physical (e.g., hydrogen bonds, entanglement of chains, ionic gelation, and van der Waals interactions) or chemical (simple monomers reaction -covalent bonds) cross-linking of molecules, as depicted in Figure 6. These characteristics allow water absorption and retention in the interstitial spaces between chains, which increase their volume and potentiate a multitude of different mechanical behaviours, making them an interesting and potentially intelligent (i.e., reactive to external stimuli) material to be used as controlled release systems of bioactive molecules (Amiriana et al, 2021;Pettinelli et al, 2020). Despite their highly hydrophilic nature, HGs allow the incorporation of hydrophobic compounds such as CUR, into the gel matrix, encapsulating and protecting those until they reach a specific target within the human body (e.g., mouth, stomach, liver, prostate, small intestine, and colon).…”

Section: Hydrogelsmentioning

confidence: 99%

Curcumin encapsulation in nanostructures for cancer therapy: A 10-year overview

D'Angelo

Noronha

Kurnik

et al. 2021

International Journal of Pharmaceutics

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

In-situ crosslinked hydrogel based on amidated pectin/oxidized chitosan as potential wound dressing for skin repairing

Cited by 155 publications

References 56 publications

Differential Responses to Bioink-Induced Oxidative Stress in Endothelial Cells and Fibroblasts

Differential Responses to Bioink-Induced Oxidative Stress in Endothelial Cells and Fibroblasts

Multifunctional Injectable Hydrogel Loaded with Cerium-Containing Bioactive Glass Nanoparticles for Diabetic Wound Healing

Curcumin encapsulation in nanostructures for cancer therapy: A 10-year overview

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