Differential Responses to Bioink-Induced Oxidative Stress in Endothelial Cells and Fibroblasts

Genç, Hatice; Hazur, Jonas; Karakaya, Emine; Dietel, Barbara; Bider, Faina; Groll, Jürgen; Boccaccını, Aldo R.; Detsch, Rainer; Cicha, Iwona

doi:10.3390/ijms22052358

Cited by 16 publications

(34 citation statements)

References 54 publications

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“…This could be either because the hydrogel itself is more stable the higher the molecular weight, as seen in the degradation experiments or it could be due to the fact that smaller chains can be more easily taken up by the cells, so that the aldehyde groups are located inside the cells, where they can have a detrimental effect on the viability. [ 47 ]…”

Section: Resultsmentioning

confidence: 99%

Hydrogels Based on Oxidized Cellulose Sulfates and Carboxymethyl Chitosan: Studies on Intrinsic Gel Properties, Stability, and Biocompatibility

Willems

Trutschel

Mazaikina

et al. 2021

Macromolecular Bioscience

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Cellulose and chitosan are excellent components for the fabrication of bioactive scaffolds, as they are biocompatible and abundantly available. Their derivatives Ocarboxymethyl chitosan (CMChi) and oxidized cellulose sulfate (oxCS) can form in situ gelling, bioactive hydrogels, due to the formation of imine bonds for crosslinking. Here the influence of the degrees of sulfation (DS), oxidation (DO), and the molecular weight of oxCS on intrinsic and rheological properties of such hydrogels and their ability to support the survival and growth of human‐adipose‐derived stem cells (hADSC) is investigated. It is found that the pH of the hydrogels is generally slightly acidic, while their network density and E‐modulus are found to be dependent on the DS and DO, which makes the properties of hydrogels tunable. Extensive studies show that hydrogels can be stable for up to 14 days and that their stability is largely dependent on the DO, molecular weight, and the components mixing ratio. Cytotoxicity studies of the hydrogel with hADSCs show biocompatible gels in dependence on the molecular weight and degree of oxidation with viable cells up to 14 days. These findings can help to develop specifically tailored hydrogels for tissue engineering applications to replace different types of connective tissue.

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Section: Resultsmentioning

confidence: 99%

Hydrogels Based on Oxidized Cellulose Sulfates and Carboxymethyl Chitosan: Studies on Intrinsic Gel Properties, Stability, and Biocompatibility

Willems

Trutschel

Mazaikina

et al. 2021

Macromolecular Bioscience

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“…They demonstrate that the attachment, proliferation, spreading and viability of human dermal hFBs were significantly enhanced on ADA-GEL hydrogels synthesized by covalent crosslinking of ADA and gelatin, as in our studies. A reason for the decrease in proliferation in the hydrogels with higher percentage of oxidation (20%) was explained by Genç et al [66]. They studied alginate, ADA and ADA-GEL, and their effect on cells (fibroblasts and endothelial cells), and they showed that there was a concentration-dependent effect of the degree of oxidation on cell viability.…”

Section: Proliferation and Live/dead Assays: Encapsulated Hfbsmentioning

confidence: 97%

Preparation and Characterization of Plasma-Derived Fibrin Hydrogels Modified by Alginate di-Aldehyde

Sanz-Horta

Matesanz

Jorcano

et al. 2022

IJMS

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Fibrin hydrogels are one of the most popular scaffolds used in tissue engineering due to their excellent biological properties. Special attention should be paid to the use of human plasma-derived fibrin hydrogels as a 3D scaffold in the production of autologous skin grafts, skeletal muscle regeneration and bone tissue repair. However, mechanical weakness and rapid degradation, which causes plasma-derived fibrin matrices to shrink significantly, prompted us to improve their stability. In our study, plasma-derived fibrin was chemically bonded to oxidized alginate (alginate di-aldehyde, ADA) at 10%, 20%, 50% and 80% oxidation, by Schiff base formation, to produce natural hydrogels for tissue engineering applications. First, gelling time studies showed that the degree of ADA oxidation inhibits fibrin polymerization, which we associate with fiber increment and decreased fiber density; moreover, the storage modulus increased when increasing the final volume of CaCl2 (1% w/v) from 80 µL to 200 µL per milliliter of hydrogel. The contraction was similar in matrices with and without human primary fibroblasts (hFBs). In addition, proliferation studies with encapsulated hFBs showed an increment in cell viability in hydrogels with ADA at 10% oxidation at days 1 and 3 with 80 µL of CaCl2; by increasing this compound (CaCl2), the proliferation does not significantly increase until day 7. In the presence of 10% alginate oxidation, the proliferation results are similar to the control, in contrast to the sample with 20% oxidation whose proliferation decreases. Finally, the viability studies showed that the hFB morphology was maintained regardless of the degree of oxidation used; however, the quantity of CaCl2 influences the spread of the hFBs.

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“…Some applied photoactive crosslink for establishing alginate encapsulation, but the process led to obvious toxicity since plenty of redox was generated [99]. Incorporation of hydroxyl and aldehyde groups into alginate is another way to stabilize encapsulation, as well as retain permeability [100,101].…”

Section: Alginatementioning

confidence: 99%

Layer-by-Layer Cell Encapsulation for Drug Delivery: The History, Technique Basis, and Applications

Lei

Feng

et al. 2022

Pharmaceutics

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The encapsulation of cells with various polyelectrolytes through layer-by-layer (LbL) has become a popular strategy in cellular function engineering. The technique sprang up in 1990s and obtained tremendous advances in multi-functionalized encapsulation of cells in recent years. This review comprehensively summarized the basis and applications in drug delivery by means of LbL cell encapsulation. To begin with, the concept and brief history of LbL and LbL cell encapsulation were introduced. Next, diverse types of materials, including naturally extracted and chemically synthesized, were exhibited, followed by a complicated basis of LbL assembly, such as interactions within multilayers, charge distribution, and films morphology. Furthermore, the review focused on the protective effects against adverse factors, and bioactive payloads incorporation could be realized via LbL cell encapsulation. Additionally, the payload delivery from cell encapsulation system could be adjusted by environment, redox, biological processes, and functional linkers to release payloads in controlled manners. In short, drug delivery via LbL cell encapsulation, which takes advantage of both cell grafts and drug activities, will be of great importance in basic research of cell science and biotherapy for various diseases.

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Differential Responses to Bioink-Induced Oxidative Stress in Endothelial Cells and Fibroblasts

Cited by 16 publications

References 54 publications

Hydrogels Based on Oxidized Cellulose Sulfates and Carboxymethyl Chitosan: Studies on Intrinsic Gel Properties, Stability, and Biocompatibility

Hydrogels Based on Oxidized Cellulose Sulfates and Carboxymethyl Chitosan: Studies on Intrinsic Gel Properties, Stability, and Biocompatibility

Preparation and Characterization of Plasma-Derived Fibrin Hydrogels Modified by Alginate di-Aldehyde

Layer-by-Layer Cell Encapsulation for Drug Delivery: The History, Technique Basis, and Applications

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