Multifunctional Injectable Hydrogel Loaded with Cerium-Containing Bioactive Glass Nanoparticles for Diabetic Wound Healing

Abstract

Diabetic foot wound healing is a major clinical problem due to impaired angiogenesis and bacterial infection. Therefore, an effective regenerative dressing is desiderated with the function of promoting revascularization and anti-bacteria. Herein, a multifunctional injectable composite hydrogel was prepared by incorporation of the cerium-containing bioactive glass (Ce-BG) into Gelatin methacryloyl (GelMA) hydrogel. The Ce-BG was synthesized by combining sol-gel method with template method, which maintained spherical shape, chemical structure and phase constitution of bioactive glass (BG). The Ce-BG/GelMA hydrogels had good cytocompatibility, promoted endothelial cells migration and tube formation by releasing Si ion. In vitro antibacterial tests showed that 5 mol % CeO2-containing bioactive glass/GelMA (5/G) composite hydrogel exhibited excellent antibacterial properties. In vivo study demonstrated that the 5/G hydrogel could significantly improve wound healing in diabetic rats by accelerating the formation of granulation tissue, collagen deposition and angiogenesis. All in all, these results indicate that the 5/G hydrogel could enhance diabetic wound healing. Therefore, the development of multifunctional materials with antibacterial and angiogenic functions is of great significance to promote the repair of diabetic wound healing.

Polymer-Based Wound Dressing Materials Loaded with Bioactive Agents: Potential Materials for the Treatment of Diabetic Wounds

“…These hydrogels significantly reduced colony numbers of both S. aureus and E. coli, revealing their potential for the treatment of bacterial-infected diabetic wounds. The in vivo wound healing studies using the full-thickness skin defect model of diabetic showed that the wound closure time in the groups of injectable hydrogels loaded with nanoparticles was faster than the blank hydrogels and the control group [118]. Shi et al, prepared chitosan-dextran hydrogels loaded with Ag nanoparticles for diabetic wound treatment.…”

Preparation of Recombinant Human Collagen III Protein Hydrogels with Sustained Release of Extracellular Vesicles for Skin Wound Healing

“…CD31 is a transmembrane protein expressed in early angiogenesis, indicating neovascularization, while α-SMA is a cytoplasmic protein expressed in later angiogenesis, indicating the maturation of vascular smooth muscle cells [ 34 ]. The vascular network plays a key role in the repair of full-thickness skin due to its function of transporting nutrients, oxygen and immune cells into the wound area [ 26 ].…”

Multifunctional mesoporous silica-cerium oxide nanozymes facilitate miR129 delivery for high-quality healing of radiation-induced skin injury

“…Ma [ 24 ] has suggested that, by combining the inherent antibacterial activity of CeO 2 -NPs, MoS 2 -CeO 2 nanocomposites exhibit excellent antibacterial ability for photothermal therapy under 808 nm laser irradiation against both G + and G − bacteria, thereby reducing the risk of wound infection. In vitro antibacterial tests have shown that a 5-mol% CeO 2 bioactive glass/gelma hydrogel composite exhibited good antibacterial properties [ 11 ].…”

“…CeO 2 -NPs have a cubic fluorite structure with surfaces of intergrown Ce 3+ and Ce 4+ . The main advantage of ceria (CeO 2 ) is the creation of oxygen (O) vacancies in the crystal lattice [ 11 ]. Therefore, the redox properties of CeO 2 -NPs have been improved, contributing to the treatment of various oxidative stress-related diseases.…”