In silico prediction of interactions and molecular dynamics simulation analysis of Mpro of Severe Acute Respiratory Syndrome caused by novel coronavirus 2 with the FDA-approved nonprotein antiviral drugs

Sivakumar, Subramaniam; Mohanasundaram, Sugumar; Rangarajan, Narasimhan; Sampath, Venkatesan; Prakash, Maderi Velayutham Dass

doi:10.7324/japs.2022.120508

Cited by 15 publications

(16 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In cell viability assay, MG-63 visible cells were harvested and were counted using a hemocytometer and diluted in Dulbecco`s Modified Eagle Media (DMEM) to a density of 1 × 10 4 cells/ml and were seeded in 96 well plates for every well and allowed for attachment. After 24h of incubation, it had been treated with control and with S1 and S2 at various concentrations (5,10,15,20,25,30,35, and 40 µg/well), which are incubated at 37 °C during a humidified 95% air and 5% CO2 incubator for twenty-four hours. After incubation, the drug-containing cells were washed with a fresh culture medium, and each well was incubated for an additional 4 h at 37 °C with 5 mg/ml MTT in PBS.…”

Section: Cell Cytotoxicity (Mtt Assay)mentioning

confidence: 99%

“…The Schiff base reaction occurs when aldehydes (or ketones) combine with amino acids, forming imine groups (C=N). Schiff bases of heterocyclic aldehydes and amino acids containing nitrogen, oxygen, and sulphur atoms are a class of compounds that exhibit significant biological activity [24], including antiviral [25], antifungal [26], antioxidant [27], anti-inflammatory [28], antitumor [29,30], anticancer [31,32], antibacterial [33,34], and antipyretic applications [11,35]. Several biological activities such as cytotoxicity studies, antibacterial, antifungal, anti-tuberculosis, DNA binding, antioxidant, scavenging, and antiviral activities have been performed [36] using isoniazid Schiff bases.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isoniazid-Based Schiff's Bases in Bone Cancer Studies Using Mg-63 Cell Lines

2022

View full text Add to dashboard Cite

Objective: Anticancer activities of Schiff bases S1 and S2 against MG-63 human osteosarcoma cells were performed and described using an in vitro evaluation employing cytotoxicity and apoptosis assay. Methods: MG-63 cells were used in an MTT assay to examine the effect of the compound on cell viability (S1 and S2). Cell morphologies and IC50 values were obtained. The acridine orange (AO)/ethidium bromide (EB) dual staining technique was used to determine the apoptosis process. Results: Our findings showed that synthesised S1 and S2 reduced MG-63 cell proliferation and induced apoptosis in a dose-dependent manner, implying that they could be used to treat bone cancer. Conclusion: Our findings showed that synthesised S1 and S2 reduced MG-63 cell proliferation and induced apoptosis in a dose-dependent manner, implying that they could be used to treat bone cancer.

show abstract

Section: Cell Cytotoxicity (Mtt Assay)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isoniazid-Based Schiff's Bases in Bone Cancer Studies Using Mg-63 Cell Lines

2022

View full text Add to dashboard Cite

show abstract

“…Proteins overexpressed in the body may affect carbapenem cleavage, modification, and transport. This could lead to carbapenem resistance as a result [21,22].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Study of Carbapenem-Resistant K. Pneumoniae Clinical Isolates

SUBRAMANIYAN

Dhanaraj

2022

Int J App Pharm

View full text Add to dashboard Cite

Objective: Now it's a worldwide issue that carbapenem resistance is spreading. This has made K. pneumoniae infections more difficult to treat. All Klebsiella pneumonia's proteins were examined in this study, which focused on the carbapenem-resistant bacteria's response to meropenem. Methods: Proteomics (MALDI-TOF) and bioinformatics methods were combined to answer the new enigma of resistance. Functional annotation, pathway enrichment and protein–protein interaction were some of the uses of this data. Both KEGG and STRING played an important role (PPI). Results: Proteins that help synthesise DNA and RNA, proteins that aid in carbapenem degradation, and proteins that aid energy and intermediate metabolism are all subdivided into two classes.007A Conclusion: Bacterial survival and meropenem resistance may have been aided by four overexpressed proteins and their partners. A new anti-resistance medication based on these proteins could help restrict the growth of "bad bugs."

show abstract

“…The extract of the plant's roots in ethyl acetate was shown to be the most efficient against the target fungus species. M. gypseum (78.83 percent inhibition of mycelial growth), M. fulvum (62.33 percent inhibition of mycelial growth), and M. canis (42.30 percent inhibition of mycelial growth) were observed after 24 h of incubation with ethyl acetate at a test concentration of 1000 g/ml [40].…”

Section: Anti-fungal Activitymentioning

confidence: 98%

Therapeutic and Pharmacological Efficacy of Plant Boerhaavia Diffusa–a Review

BHUMINATHAN

REKHA³

et al. 2022

Int J App Pharm

View full text Add to dashboard Cite

Boerhavia diffusa (BD) is a herb of rasayana type, according to ayurvedic statements. It is considered to offer anti-aging, anti-disease, and life-strengthening qualities. Diffusa Boerhaavia Linn. Contains a wide variety of therapeutic qualities that have been demonstrated to be beneficial in the treatment of hyperglycemia, inflammation, anxiety, hepatotoxicity, jaundice, and heart problems. Here, we focus on the chemical composition and ethnomedicinal uses of B. diffusa that extend back to ancient times, as well as their future development. B. diffusa's exceptional antioxidant, hepatoprotective, antibacterial, anti-diabetic, and anticarcinogenic capabilities have drawn pioneers in science and medicine. Additionally, this herb possesses medicinal properties, which is a result of the presence of polyphenols and flavanoids, creates it more vital for clinicians and scientists to exploit in order to obtain a better understanding of its biological and pharmacological capabilities.

show abstract

In silico prediction of interactions and molecular dynamics simulation analysis of Mpro of Severe Acute Respiratory Syndrome caused by novel coronavirus 2 with the FDA-approved nonprotein antiviral drugs

Cited by 15 publications

References 0 publications

Isoniazid-Based Schiff's Bases in Bone Cancer Studies Using Mg-63 Cell Lines

Isoniazid-Based Schiff's Bases in Bone Cancer Studies Using Mg-63 Cell Lines

Proteomic Study of Carbapenem-Resistant K. Pneumoniae Clinical Isolates

Therapeutic and Pharmacological Efficacy of Plant Boerhaavia Diffusa–a Review

Contact Info

Product

Resources

About