Isoniazid-Based Schiff's Bases in Bone Cancer Studies Using Mg-63 Cell Lines

Abstract

Objective: Anticancer activities of Schiff bases S1 and S2 against MG-63 human osteosarcoma cells were performed and described using an in vitro evaluation employing cytotoxicity and apoptosis assay. Methods: MG-63 cells were used in an MTT assay to examine the effect of the compound on cell viability (S1 and S2). Cell morphologies and IC50 values were obtained. The acridine orange (AO)/ethidium bromide (EB) dual staining technique was used to determine the apoptosis process. Results: Our findings showed that synthesised S1 and S2 reduced MG-63 cell proliferation and induced apoptosis in a dose-dependent manner, implying that they could be used to treat bone cancer. Conclusion: Our findings showed that synthesised S1 and S2 reduced MG-63 cell proliferation and induced apoptosis in a dose-dependent manner, implying that they could be used to treat bone cancer.

Selective Detection of Copper Ions and Biological Activities of Isoniazid Schiff Bases

“…1 H and 13 C spectra of S2 were also reported in our previous work [17] . 1 H : The H 1 NMR spectral data of compound S2 are consistent with the literature.…”

“…[40] 1 H and 13 C spectra of S1 were also reported in our previous work. [17] 1 H: The 1 H NMR spectral data of the ligand S1 agreed with the literature data. E isomerism of the compound, N'-((1H-Pyrrol-2-yl)methylene)isonicotinohydrazide, Scheme 1, was confirmed with the theoretical 1 H NMR chemical shift values obtained with ChemDraw 12.0 with the observed experimental values (Table 1).…”

“…Synthesis of S2. [17] ChemistrySelect tional chemical quantum calculations were carried out. [35] With the aid of the GaussView 5.0 program, the structures and electron density charts are produced.…”

“…Due to the greater therapeutic importance of INH, it is used against bacterial infections [18] in a broad spectrum. INH is used an effective treatment for osteosarcoma which is a type of cancer that is more common in adolescents and adults, with the lungs being the primary site for more than 80 % of individuals with osteosarcoma metastasis [17,19–20] . In general, different derivatives of INH based compounds were already studied for anti‐mycobacterial studies which is presented in Figure 1 [21] …”

“…In this work, as a part of or continuing research about INH ligands, their complexes, and their biological studies, [17] we have synthesized INH-based Schiff bases named (E)-N'-((1Hpyrrol-2-yl)methylene)isonicotinohydrazide (S1) and (E)-N'-(thiophen-2-ylmethylene) isonicotinohydrazide (S2), within which S1…”