In rats with estradiol valerate-induced polycystic ovary syndrome, the acute blockade of ovarian β-adrenoreceptors improve ovulation

Venegas, Berenice; Gordillo, Lizzbeth Yureli De León; Rosas, Gabriela; Espinoza, Julieta A.; Morán, Carolina; Domı́nguez, Roberto; Morales‐Ledesma, Leticia

doi:10.1186/s12958-019-0539-y

Cited by 22 publications

(15 citation statements)

References 52 publications

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“…On the other hand, according to the inflammation hypothesis, the ovulation-related events have been reported to resemble an inflammatory reaction that accompany with leukocytes infiltration and production of inflammatory mediators like cytokines, vascular endothelial growth factor (Vascular Endothelial Growth Factor, VEGF), prostaglandins, and intracellular signaling pathways closely associated with inflammatory reaction [ 75 ]. Regarding the results shown in Table 1 , the laying hens, supplemented by BAA Salmeterol and BB Propranolol, significantly indicated more ovulation rate and the similar follicle size F1 to F5 as compared to control that was in agreement with the studies which showed catecholamines and Propranolol improve the ovulation rate and follicular development [ 76 , 77 ]. Besides, Fig.…”

Section: Discussionsupporting

confidence: 87%

The stimulation and inhibition of beta-2 adrenergic receptor on the inflammatory responses of ovary and immune system in the aged laying hens

et al. 2021

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Background Ovarian chronic inflammation has been known to incidence in the laying hen mainly via increasing laying frequency and microbial infection, especially during late stage of production period. This study was aimed to evaluate beta-2 adrenergic agonist (Beta-2 Adrenergic Agonist, BAA) Salmeterol and beta blocker (Beta Blocker, BB) Propranolol on the gene expression of the ovarian pro- and anti-inflammatory mediators, inflammatory responses of immune system, ovarian functions and, hormones in the laying hens on the late stage of production period. Forty-eight White Leghorn hens aged 92 weeks were used for 4 weeks to be supplemented by Salmeterol and Propranolol. Ovulation rate and follicular growth were determined based on laying frequency and ovarian visual evaluation, respectively; the mRNA expressions of follicular beta-2 adrenergic receptor (Beta-2 Adrenergic Receptor, β2ADR), cyclooxygenases (Cyclooxygenases, COX) 1 and 2, and cytokines were measured by real-time PCR. The plasma concentration of ovarian hormones, cellular, and humoral immune responses were measured via ELISA, heterophil to lymphocyte ratio (Heterophil to Lymphocyte ratio, H:L), and sheep red blood cell (Sheep Red Blood Cell, SRBC) test, respectively. Results As compared to control, both of BAA Salmeterol and BB Propranolol resulted in a significant decrease in the mRNA expression of β2ADR, cyclooxygenases, and pro- and anti-inflammatory cytokines (P < 0.01). A significant elevation was observed in the ovulation rate (P < 0.05), plasma estradiol content on both treated groups (P < 0.05), and the content of progesterone and was just significantly (P < 0.05) increased in Salmeterol group. H:L was reduced in BAA group (P < 0.05), and immunoglobulin (Ig) M was elevated in both treated hens, when compared to control. The results indicated that Salmeterol significantly increases body weight (P < 0.05). Conclusion The stimulation and inhibition of beta-2 adrenergic signaling could reduce ovarian inflammatory condition in addition to enhancing laying efficiency in the aged laying hens.

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Section: Discussionsupporting

confidence: 87%

The stimulation and inhibition of beta-2 adrenergic receptor on the inflammatory responses of ovary and immune system in the aged laying hens

et al. 2021

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“…An experimental study conducted by Pournaderi et al [ 40 ] found the same results, indicating that after EV-induced PCOS, the animals presented decreased levels of LH, FSH, and estradiol and also found increased levels of progesterone in comparison with the control group. Venegas et al [ 41 ] similarly found that increased serum levels of progesterone. Laird et al [ 42 ], in an in vitro study, examined the role of testosterone on the transition of preantral follicles to the antral form.…”

Section: Discussionmentioning

confidence: 99%

Alteration of TGFB1, GDF9, and BMPR2 gene expression in preantral follicles of an estradiol valerate-induced polycystic ovary mouse model can lead to anovulation, polycystic morphology, obesity, and absence of hyperandrogenism

Asghari

Shokri‐Asl

Rezaei

et al. 2021

Clin Exp Reprod Med

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Objective: In humans, polycystic ovary syndrome (PCOS) is an androgen-dependent ovarian disorder. Aberrant gene expression in folliculogenesis can arrest the transition of preantral to antral follicles, leading to PCOS. We explored the possible role of altered gene expression in preantral follicles of estradiol valerate (EV) induced polycystic ovaries (PCO) in a mouse model.Methods: Twenty female balb/c mice (8 weeks, 20.0±1.5 g) were grouped into control and PCO groups. PCO was induced by intramuscular EV injection. After 8 weeks, the animals were killed by cervical dislocation. Blood serum (for hormonal assessments using the enzyme-linked immunosorbent assay technique) was aspirated, and ovaries (the right ovary for histological examinations and the left for quantitative real-time polymerase) were dissected. Results: Compared to the control group, the PCO group showed significantly lower values for the mean body weight, number of preantral and antral follicles, serum levels of estradiol, luteinizing hormone, testosterone, and follicle-stimulating hormone, and gene expression of TGFB1, GDF-9 and BMPR2 (p<0.05). Serum progesterone levels were significantly higher in the PCO animals than in the control group (p<0.05). No significant between-group differences (p>0.05) were found in BMP6 or BMP15 expression. Conclusions: In animals with EV-induced PCO, the preantral follicles did not develop into antral follicles. In this mouse model, the gene expression of TGFB1, GDF9, and BMPR2 was lower in preantral follicles, which is probably related to the pathologic conditions of PCO. Hypoandrogenism was also detected in this EV-induced murine PCO model.

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“…The first ten animals were left without PCOS induction and were treated for 20 days with a normal saline. The rest thirty females were given a single intramuscular injection of 4 mg estrogen valerate (Riedeldehaen, Germany) to induce PCOS [9][10]. After 30 days of induction of PCOS, the animals were divided into three groups (10 each) and treated respectively with cyproterone 12 mg/ kg, metformin 50 mg/kg as a single oral dose daily, while the third group was given normal saline (the vehicle) 0.5 ml/animal to serve as negative control (PCO untreated group).…”

Section: Methodsmentioning

confidence: 99%

Study the efficacy of cyproterone and metformin in modulation of hormonal disturbances in estrogen valerate induced PCOS in female rats

Majid¹,

Talab²,

Saeed³

2021

Int. J. Biol. Pharm. Sci. Arch.

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Polycystic ovarian syndrome (PCOS) is an endocrine disorder affecting women in child bearing age and is considered the main cause of infertility. This study aims to determine the efficacy of cyproterone 12 mg/ kg and metformin 50 mg/kg as a single oral daily dose for 20 days, in PCOS induced in female rats by estrogen valerate. In untreated group PCOS, the level of serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH) was significantly (P< 0.0001) decrease, while estradiol and testosterone levels were increased significantly (P< 0.0001 and P< 0.001 respectively) in comparison with control group. Both cyproterone and metformin significantly increased LH and FSH levels (P< 0.05) compared to the untreated PCOS group. Both cyproterone and metformin reduced the level of estradiol hormone significantly (P <0.001 and P <0.05, respectively). Cyproterone also decreased the level of testosterone (P <0.0001) compared to the untreated PCOS group, and the testosterone level became less than that recorded in the control group (P <0.05), Metformin also significantly decreased the level of testosterone compared to the untreated PCOS group (P <0.01), but it still more than that in control. According to the results of the current study, we can conclude that cyproterone and metformin showed an efficacy in the treatment of PCO, and can restore the normal hormonal levels and should be considered in the treatment of this disease.

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In rats with estradiol valerate-induced polycystic ovary syndrome, the acute blockade of ovarian β-adrenoreceptors improve ovulation

Cited by 22 publications

References 52 publications

The stimulation and inhibition of beta-2 adrenergic receptor on the inflammatory responses of ovary and immune system in the aged laying hens

The stimulation and inhibition of beta-2 adrenergic receptor on the inflammatory responses of ovary and immune system in the aged laying hens

Alteration of TGFB1, GDF9, and BMPR2 gene expression in preantral follicles of an estradiol valerate-induced polycystic ovary mouse model can lead to anovulation, polycystic morphology, obesity, and absence of hyperandrogenism

Study the efficacy of cyproterone and metformin in modulation of hormonal disturbances in estrogen valerate induced PCOS in female rats

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