In-House Validation of an Efficient and Rapid Procedure for the Simultaneous Determination and Monitoring of 23 Mycotoxins in Grains in Korea

Abstract

A high-performance liquid chromatography tandem mass spectrometry method is described for the simultaneous determination of mycotoxins, including Ergot alkaloids (EAs), in 3 types of grains. The extraction of 23 mycotoxins was evaluated and performed by using a modified QuEChERS-based sample preparation procedure. The proposed method was fully validated on spiked grain samples (barley, wheat and oat) to assess the linearity, limit of detection (LOD) and limit of quantitation (LOQ), matrix effects, precision and recovery. After validation, this method was applied to 143 samples of various types of 3 grains from the Republic of Korea to survey the level of mycotoxin contamination in Republic of Korean grains. A total of 42 grain samples (29%) were contaminated with at least one of these mycotoxins at levels higher than the LOQ. The results demonstrated that the procedure was suitable for simultaneously determining these mycotoxins in cereals and could be performed for their routine analysis in mycotoxin laboratories.

Simultaneous determination of 78 pesticide residues and 16 mycotoxins in tsampa by an improved QuEChERS method coupled with ultra performance liquid chromatography-tandem mass spectrometry

“…The results, as shown in Table 4, showed that the LOQs of the present method for pesticide residues were significantly lower than those of ref. 41–43. Meanwhile, for the detection of mycotoxins, the LOQs of the present method were all less than or equal to those of ref.…”

T-2 and HT-2 Toxins: Toxicity, Occurrence and Analysis: A Review

“…As well as extracting the analytes of choice from the matrix, one of the main issues is the co-extraction of matrix compounds and the associated matrix effects, which can lead to issues with the analysis. These include, but are not limited to: signal suppression or enhancement (SSE), poor chromatography, false positives due to the presence of isobaric compounds and poor or incomplete recovery of the target analytes [ 126 ]. Therefore, after the initial extraction, a sample clean-up step is usually employed in order to remove as much matrix as possible before analysis, which also serves to decrease the limit of detection (LOD) and limit of quantification (LOQ) should this be a requirement.…”

Recent Advances in Mycotoxin Determination in Fish Feed Ingredients

“…The remaining water and other interfering substances from the matrix are eliminated in this stage [ 65 ]. There are several methods using the QuEChERS protocols for the extraction and clean-up of mycotoxins before instrumental analysis [ 66 , 67 , 68 , 69 , 70 , 71 , 72 ].…”