A comparative study on the effect of fish meal substitution with three different insect meals on growth, body composition and metabolism of European sea bass (Dicentrarchus labrax L.

The determination of organic and inorganic pollutants in fish samples is a complex and demanding process, due to their high protein and fat content. Various novel sorbents including graphene, graphene oxide, molecular imprinted polymers, carbon nanotubes and metal-organic frameworks (MOFs) have been reported for the extraction and preconcentration of a wide range of contaminants from fish tissue. MOFs are crystalline porous materials that are composed of metal ions or clusters coordinated with organic linkers. Those materials exhibit extraordinary properties including high surface area, tunable pore size as well as good thermal and chemical stability. Therefore, metal-organic frameworks have been recently used in many fields of analytical chemistry including sample pretreatment, fabrication of stationary phases and chiral separations. Various MOFs, and especially their composites or hybrids, have been successfully utilized for the sample preparation of fish samples for the determination of organic (i.e., antibiotics, antimicrobial compounds, polycyclic aromatic hydrocarbons, etc.) and inorganic pollutants (i.e., mercury, palladium, cadmium, lead, etc.) as such or after functionalization with organic compounds.

White sea bream (Diplodus sargus) and brown meagre (Sciaena umbra) were caught from the wild in Aegean Sea (Greece), and studied for their proximate, fatty acid and amino acid composition to evaluate their nutritional value for human consumption and their potential as candidate fish species for Mediterranean aquaculture diversification. Both species exhibited very low muscle fat, ranging at 1%. White sea bream was found to have higher muscle eicosapentaenoic (EPA) and docohexaenoic (DHA) contents and total n-3 fatty acids than brown meagre. A superiority of white sea bream in aspects of thrombogenicity was indicated, showing higher atherogenic (0.679) and thrombogenic indices (0.377) comparing to the respective values for brown meagre (0.610 and 0.579). The two-studied species exhibited high-quality protein as food source, with white sea bream showing a higher essential to non-essential amino acid (EAA/NEAA) rate, 0.764 vs 0.704, respectively. The individual to total essential amino acids rates of lysine and leucine were the highest ones for both studied species, while no significant differences were observed among them.

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Low-cost plant-based sources used in aquaculture diets are prone to the occurrence of animal feed contaminants, which may in certain conditions affect the quality and safety of aquafeeds. Mycotoxins, a toxic group of small organic molecules produced by fungi, comprise a frequently occurring plant-based feed contaminant in aquafeeds. Mycotoxin contamination can potentially cause significant mortality, reduced productivity, and higher disease susceptibility; thus, its timely detection is crucial to the aquaculture industry. The present review summarizes the methodological advances, developed mainly during the past decade, related to mycotoxin detection in aquafeed ingredients, namely analytical, chromatographic, and immunological methodologies, as well as the use of biosensors and spectroscopic methods which are becoming more prevalent. Rapid and accurate mycotoxin detection is and will continue to be crucial to the food industry, animal production, and the environment, resulting in further improvements and developments in mycotoxin detection techniques.

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