In house validation of an HPLC-DAD method for the simultaneous determination of specific bioactive compounds in southern Brazilian propolis

Machado, Christiane Schineider; Finger, Daiane; Felsner, Maria Lurdes; Torres, Yohandra Reyes

doi:10.1080/00218839.2018.1501857

Cited by 7 publications

(9 citation statements)

References 28 publications

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“…Forty-four samples of brown propolis ( Apis mellifera ) were supplied by beekeepers from the state of Paraná (southern Brazil) in the summer of 2011 (Table ). Representative samples of propolis from other Brazilian states were studied: 02 green propolis (01 from São Paulo, 01 from Minas Gerais), 01 red propolis (Bahia), and 01 brown propolis (Santa Catarina). , All samples were stored at −18 °C until they were extracted. Each propolis sample (1 g) was extracted with 50 mL of 95% (v/v) ethanol in an ultrasonic water bath for 20 min at room temperature and then filtered.…”

Section: Materials and Methodsmentioning

confidence: 99%

“…Aliquots were taken from stock solutions to prepare a solution containing a mix of the nine standards at the final concentration of 100 μg/mL. This mix was used to develop and validate the analytical procedure …”

Section: Materials and Methodsmentioning

confidence: 99%

“…HPLC was carried out in a Waters 600 system using a C 18 column (XTerra, 4.6 mm × 250 mm, 5 μm) maintained at 30 °C . The mobile phase consisted of acetonitrile (A) and 0.5% formic acid in water (B).…”

Section: Materials and Methodsmentioning

confidence: 99%

“…As part of our efforts to characterize the propolis produced in the state of Paraná (Brazil), we have previously carried out a meticulous quantitative evaluation, supported through analytical validation, of individual compounds in Brazilian propolis . Our results showed that p -coumaric, 3,5-dicaffeoylquinic, 3,4-di- O- caffeoylquinic, and 4- O -caffeoylquinic acids are important constituents of most brown propolis samples of the state of Paraná.…”

Section: Introductionmentioning

confidence: 95%

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Seeking the Regional Identity of Brown Propolis Produced in Southern Brazil and Linked to Total Levels of Bioactive Compounds and Biological Activity

Machado

Finger

Lima

et al. 2021

ACS Food Sci. Technol.

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Total levels of caffeoylquinic, p-coumaric, and benzoic acid derivatives, flavonols, and dihydroflavonols were investigated in 44 brown propolis samples from the state of Paraná(Brazil) together with their cytotoxicity and antibacterial activities. The aim was to discover chemical and biological activity-based patterns. Great quantitative variation was found within individual samples; however, a typical extract might contain 11.14−21.45 mg of caffeoylquinic acid derivatives/g, 6.27−12.17 mg of p-coumaric acid derivatives/g, 3.18−7.45 mg of benzoic acid derivatives/g, 9.35−23.55 mg of flavonols/g, and 0.17−4.25 mg of dihydroflavonols/g. MIC values poorly correlated to the levels of p-coumaric acids, flavonols, and dihydroflavonols. Most propolis samples showed medium-to-high cytotoxic activity against at least one tumoral cell. The most susceptible cell was OVCAR-8, followed by SF-295, but no correlation was found between the inhibition of OVCAR-8 and SF-295 and levels of any chemical class investigated. The concentration of bioactive compounds in brown propolis samples together with biological activities distinguished samples produced in northern, southern, eastern, and western Parana, being characteristics associated with the geographical origin, and may help to establish a pattern for the propolis produced in the state of Parana.

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Section: Materials and Methodsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

See 2 more Smart Citations

Seeking the Regional Identity of Brown Propolis Produced in Southern Brazil and Linked to Total Levels of Bioactive Compounds and Biological Activity

Machado

Finger

Lima

et al. 2021

ACS Food Sci. Technol.

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“…For example, in flavonols Band I (absorption due to B-ring) is usually within 300 ˗ 380 nm and Band II (absorption due to A-ring) within 240˗280 nm. 1,10,15 Figure 1 shows the grouping of Bauhinia samples resulting from PCA of UV-Vis spectra. Altogether, the first and second principal components explain 96.20 % of the variability of the extracts.…”

Section: Multivariate Analysis Of Uv-vis and Ir Fingerprintsmentioning

confidence: 99%

Identification and Characterization of Bauhinia Species by Spectroscopic and Spectrometric Fingerprints

Pianoski¹,

Turco²,

Soares³

et al. 2020

Rev. Virtual Quim.

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Resumo: Algumas espécies de plantas do gênero Bauhinia são plantas medicinais com relevantes propriedades diuréticas, digestivas, anti-inflamatórias, analgésicas, sendo popularmente usadas no tratamento da hipertensão e do diabetes. As plantas deste gênero são conhecidas como "pata-de-vaca" devido ao formato de suas folhas; essa similaridade morfológica entre espécies e/ou subespécies torna sua diferenciação taxonômica um desafio. Buscando uma ferramenta auxiliar para discriminar espécies de Bauhinia, as impressões digitais espectroscópicas e espectrométricas dos extratos das folhas foram comparadas usando a análise de componentes principais (PCA). As amostras foram diferenciadas de acordo com o tipo (comercial ou não comercial), região de origem e espécie. A análise indicou que existem diferenças de perfil químico entre as amostras comerciais e não comerciais possivelmente originadas pela mistura de diferentes espécies de Bauhinia nas amostras comerciais. Desta forma, a PCA é uma ferramenta auxiliar na autenticação de amostras comerciais e não comerciais, rastreamento de origem e classificação taxonômica. Além disso, a atividade antirradicalar foi estatisticamente superior para amostras não comerciais (p<0,05), sugerindo que essa atividade diminui durante o armazenamento na prateleira. Some species of plants of the genus Bauhinia are medicinal plants with relevant pharmaceutical properties such as diuretic, digestive, anti-inflammatory, analgesic and are popularly used to treat hypertension and diabetes. They are widely known as "cow's foot" ("pata-de-vaca") due to the shape of their leaves; this morphological similarity among species and/or subspecies makes their taxonomic differentiation a challenge. Seeking for an auxiliary tool to discriminate Bauhinia species, spectroscopic and spectrometric fingerprints of extracts of Bauhinia leaves were compared using principal component analysis (PCA). Samples were distinguished according to their type (commercial or non-commercial), geographic origin, and specie. Multivariate data analysis indicated that there are differences between the chemical profile of commercial and non-commercial samples possibly due to the mixture of different species of Bauhinia in commercial samples. Thus, PCA may help to authenticate commercial and non-commercial samples, to trace their origin and indicate taxonomic classification. Additionally, the antiradical activity was statistically higher for non-commercial samples (p<0.05), suggesting that this activity diminishes during shelf storage.

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Influence of red propolis on the physicochemical, microbiological and sensory characteristics of tilapia (Oreochromis niloticus) salami

et al. 2022

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In house validation of an HPLC-DAD method for the simultaneous determination of specific bioactive compounds in southern Brazilian propolis

Cited by 7 publications

References 28 publications

Seeking the Regional Identity of Brown Propolis Produced in Southern Brazil and Linked to Total Levels of Bioactive Compounds and Biological Activity

Seeking the Regional Identity of Brown Propolis Produced in Southern Brazil and Linked to Total Levels of Bioactive Compounds and Biological Activity

Identification and Characterization of Bauhinia Species by Spectroscopic and Spectrometric Fingerprints

Influence of red propolis on the physicochemical, microbiological and sensory characteristics of tilapia (Oreochromis niloticus) salami

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